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Method for quickly detecting contents of plant growth regulators in vegetables

A technology for plant growth regulators and vegetables, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as the acquisition of fresh vegetable raw materials, the inconvenience of export customs clearance, changing the dosage of plant growth regulators, and affecting the safety of vegetables, etc., to achieve The effect of shortening the detection cycle, strong specificity and high sensitivity

Inactive Publication Date: 2017-02-15
CHONGQING INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, driven by interests, abuse and blindly changing the dosage of plant growth regulators have affected the safety of vegetables, and the resulting food safety problems are increasing
However, numerous testing items and a long testing cycle not only bring inconvenience to the purchase of fresh vegetable raw materials and export customs clearance, but also lead to a decline in the quality of vegetable preservation

Method used

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  • Method for quickly detecting contents of plant growth regulators in vegetables
  • Method for quickly detecting contents of plant growth regulators in vegetables
  • Method for quickly detecting contents of plant growth regulators in vegetables

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0070] The preparation of the sample shall be taken according to GB / T 8855. Take the edible part of the vegetable sample, chop it up, mix it evenly, seal it, and use it as a sample, and mark it.

[0071] Preservation of the sample: Store the sample in refrigerated storage at 0°C-4°C.

Embodiment 1

[0072] Embodiment 1 The method for rapid detection of plant growth regulator content in vegetables

[0073] 1. Instruments and conditions

[0074] (1) Liquid chromatography conditions

[0075] a) Chromatographic column: Hypersil GOLD aQ, 1.9μm, 50mm×2.1mm (inner diameter) or equivalent;

[0076] b) Injection volume: 5 μL;

[0077] c) Column temperature: 40°C;

[0078] d) See Table 2 for mobile phase and gradient elution conditions.

[0079] Table 2 mobile phase and gradient elution conditions

[0080]

[0081] (2) Mass spectrometry conditions

[0082] a) Ionization method: electrospray ionization;

[0083] b) Scanning mode: positive ion scanning;

[0084] c) Detection method: multiple reaction monitoring;

[0085] d) Electrospray voltage: 3500V;

[0086] e) Sheath gas pressure: 45Arb;

[0087] f) Auxiliary air pressure: 13Arb;

[0088] e) Purge gas pressure: 1Arb;

[0089] f) Temperature of ion transfer tube: 350°C;

[0090] g) Atomization temperature: 300°C; ...

Embodiment 2

[0119] Example 2 The method for quickly detecting the content of plant growth regulators in vegetables (with leafy vegetables, melons, solanaceous fruits, tubers, and beans as representative substrates, 6 kinds of artificially synthesized plant growth regulators were studied. Rapid and accurate analysis method of drug residues)

[0120] 1. Instruments and conditions

[0121] With embodiment 1.

[0122] 2. Solvent preparation, sample extraction, salting out, stratification and purification treatment

[0123] With embodiment 1.

[0124] 3. Correction of matrix effect

[0125] The matrix effect (ME) was evaluated by post-extraction addition method, as shown in Table 5. Except Chinese cabbage substrates for 6-benzyl adenine, five substrates showed different degrees of substrate inhibitory effect on six plant growth regulators. Therefore, it is necessary to use a blank matrix to prepare a standard working solution to correct the matrix effect during quantitative analysis.

[...

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Abstract

The invention belongs to the field of food detection and relates to a method for quickly detecting contents of plant growth regulators in vegetables by means of liquid chromatography tandem mass spectrum, in particular to a method for quickly measuring the residual amounts of six plant growth regulators, 6-benzylaminopurine, thidiazuron, forchlorfenuron, paclobutrazol, uniconazole and chlorpropham, in vegetables. The method comprises: extracting a sample with acetic acid and acetonitrile mixed liquid, layering by salting out, purifying extract by means of dispersive solid-phase extraction, detecting by means of liquid chromatography tandem mass spectrum, and quantifying by external standard method. The method has the advantages that detection cycle is shortened, detection efficiency is improved, the requirement on detection and surveillance on export vegetables is met, and technical support is also provided for ensuring safety supervision for domestic foods.

Description

technical field [0001] The invention belongs to the field of food detection, and relates to a method for rapidly detecting the content of plant growth regulators in vegetables by liquid chromatography-tandem mass spectrometry, in particular to 6-benzyl adenine, thiadizuron, and forchlorfenuron in vegetables , paclobutrazol, uniconazole and chlorpropenazole and other six kinds of rapid determination method of plant growth regulator residues. Background technique [0002] Vegetables are one of the essential foods in people's daily diet, and their quality and safety issues are not only closely related to people's health, but also related to the international competitiveness of my country's agricultural products. Plant growth regulators are a class of pesticides used to regulate plant growth and development. Due to their relatively complicated application technology, their development has not been as rapid as that of insecticides, fungicides and herbicides, and their application...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 周纯洁赵博屠大伟李红
Owner CHONGQING INST FOR FOOD & DRUG CONTROL
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