Method for metal-enzyme co-catalyzed ethanol production from honeysuckle leaves and simultaneous extraction of chlorogenic acid

Abstract

The invention relates to a method for carrying out metal-enzyme co-catalysis on lonicera japonica leaves to produce ethyl alcohol and simultaneously extracting chlorogenic acid, and belongs to the technical field of production of biomass ethyl alcohol and chlorogenic acid series products. According to the method, solid wastes of the lonicera japonica leaves are used as raw materials; prepared manganese zinc ferrite is an enzyme activator; the products are obtained by a process of preparing a metal activator, culturing yeast liquid, pre-treating the raw materials, carrying out co-catalytic simultaneous glycolysis and carrying out analysis detection of the chlorogenic acid and the ethyl alcohol. The method has the characteristics that the raw materials are cheap, easy to obtain and wide in source; diversified products with complete biological activities can be obtained, and biomass resources are sufficiently utilized; energy consumption in the production process is low, corrosion to production equipment is low, and the method is beneficial to environmental protection. The method can be widely applied to simultaneous production of the ethyl alcohol by three processes of catalysis, saccharification and fermentation of biomass and extraction of natural products, such as the chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and the like, from the solid wastes of the lonicera japonica leaves, and effectively implements resource re-utilization of the lonicera japonica leaves.

Description

technical field [0001] The invention belongs to the technical field of production of biomass ethanol and chlorogenic acid series products, and specifically relates to a method for co-catalyzing and activating honeysuckle leaves with metals and enzymes to produce biomass ethanol and extracting chlorogenic acid and chlorogenic acid isomers. Background technique [0002] The production of fuel ethanol from renewable resources is one of the effective solutions to the shortage of global fossil fuels, the increase of energy costs and the reduction of carbon emissions; while biomass, with its rich carbon resources, is the only renewable energy that can be converted into liquid fuels . Biomass mainly contains cellulose, hemicellulose, lignin, etc. The biggest technical difficulty in converting it to ethanol is the degradation of natural crystalline cellulose, and the extraction of natural products with high medicinal activity in biomass is also limited by the fibrous tissue. dense ...

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: (1) This method uses 0.5-1.0% sulfuric acid solution to hydrolyze cellulose, and soaks it for 2-4 hours. During the production process, it uses environmentally polluting sulfuric acid, which increases the cost of environmental treatment, thereby increasing production costs.

(2) This method uses steam explosion to treat cellulose waste during pretreatment, and its steam explosion pressure is 1.5-2.2MPa. The pressurization technology in the production process increases the hidden danger of production and further increases the production cost

(3) Cellulase enzymatic saccharification is used, the enzymatic saccharification temperature is 45-55 ℃, the enzymatic saccharification time is 36 h, the production conditions of 45-55 ℃ require additional energy, and the cellulase saccharification is 36 h, It not only affects the enzyme activity, reduces the saccharification efficiency of biomass, but also takes a long time, increases production time and equipment occupation, and thus increases production costs

(4) This method adopts the process of cellulase saccharification and Saccharomyces cerevisiae fermentation, and in addition to using acid, steam explosion treatment is also used in the pretreatment, so multiple production equipment and production processes are involved, which increases the technical control of production and production Occupation of equipment space, thereby increasing production costs

The shortcomings of this method are: (1) other active ingredients and fibrous tissues in honeysuckle leaves are not fully utilized in this method, and the utilization rate of biomass honeysuckle leaf raw material resources cannot be improved; (2) although the method uses compound enzymes, it can Effectively degrade cellulose and lignin, with strong hydrolysis ability, but the enzyme catalysis time still needs 11 hours, and the enzyme activity needs to be increased; (3) This method is a compound enzyme with cellulase and ligninase, and the suitable pH value for its catalytic hydrolysis 4, that is, acidic conditions, which can easily cause corrosion of production equipment, thereby increasing equipment production costs

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