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Trichoderma seed coating as well as preparation method and application thereof

A technology of Trichoderma and strains, applied in the prevention and treatment of root rot, sheath blight, preparation of biological control Trichoderma biological seed coating agent, field of corn stalk rot, can solve corn lodging, few studies , Restricting the promotion of corn mechanized production technology and other issues, to achieve the effects of promoting growth indicators, broad market prospects, and improving plant stress resistance

Inactive Publication Date: 2017-02-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not only that, the high incidence of corn stalk rot caused serious corn lodging, which limited the promotion of corn mechanized production technology
Over the years, Trichoderma biocontrol agents have been mainly applied in the form of compost and granules at home and abroad. There are few reports on the successful preparation of seed coating agents from Trichoderma. The American Biowork Trichoderma biological seed coating agent is only used on corn seedlings. disease control and increase maize yield, but little research has been done on stem rot (Fusarium spp., Pythium spp.) and sheath blight (Rhizoctonia solani) that occur later in maize growth

Method used

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  • Trichoderma seed coating as well as preparation method and application thereof
  • Trichoderma seed coating as well as preparation method and application thereof
  • Trichoderma seed coating as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Plate strain activation: inoculate Trichoderma harzianum SH2303 on a PDA petri dish, and culture it upside down in a 28°C incubator for 4-5 days.

[0032] PDA medium: 200g potato boiled and filtered upper juice, 20g glucose, 20g agar powder, dilute purified water to 1L, fill each 250mL into 500mL Erlenmeyer flask, autoclave at 121℃ for 25min.

[0033] 2. Preparation of primary strains: 200g of potatoes, 20g of glucose, 1L of purified water, each 250mL was dispensed into a 500mL Erlenmeyer flask, and autoclaved at 121°C for 25min. The strains were inserted into the plate, placed in a shaker at 180 r / min, 28° C., and shaken for 5 days to obtain Trichoderma harzianum SH2303 liquid fermentation strains.

[0034] 3. Trichoderma liquid fermentation: corn flour 8kg, magnesium sulfate 80g, ferrous sulfate 1.2g, manganese sulfate 0.4g, zinc sulfate 0.32g, potassium dihydrogen sulfate 0.61kg, sodium nitrate 0.23kg, ammonium nitrate 176g, calcium chloride 160g, make up 200L wi...

Embodiment 2

[0042] 1. Plate strain activation: inoculate Trichoderma harzianum SH2303 on a PDA petri dish, and culture it upside down in a 28°C incubator for 4-5 days.

[0043] PDA medium: 200g potato boiled and filtered upper juice, 20g glucose, 20g agar powder, dilute purified water to 1L, fill each 250mL into 500mL Erlenmeyer flask, autoclave at 121℃ for 25min.

[0044] 2. Preparation of primary strains: 200g of potatoes, 20g of glucose, 1L of purified water, each 250mL was dispensed into a 500mL Erlenmeyer flask, and autoclaved at 121°C for 25min. The strains were inserted into the plate, placed in a shaking table at 180 r / min, 28° C., and shaken for 5 days to obtain Trichoderma liquid fermentation strains.

[0045] 3. Trichoderma liquid fermentation: corn flour 8kg, magnesium sulfate 80g, ferrous sulfate 1.2g, manganese sulfate 0.4g, zinc sulfate 0.32g, potassium dihydrogen sulfate 0.61kg, sodium nitrate 0.23kg, ammonium nitrate 176g, calcium chloride 160g, make up 200L with water, ...

Embodiment 3

[0056] 1. Plate strain activation: Inoculate Trichoderma viridans SH2303 on a PDA petri dish, and incubate it upside down in a 28°C incubator for 4-5 days.

[0057] PDA medium: 200g of potato boiled and filtered upper layer juice, 20g of glucose, 20g of agar powder, dilute to 1L with purified water, aliquot into 250mL Erlenmeyer flask, autoclave at 121°C for 25min.

[0058] 2. Preparation of primary strains: 200g of potatoes, 20g of glucose, 1L of purified water, aliquoted into 250mL Erlenmeyer flasks, and autoclaved at 121°C for 25min. Insert the strain on the plate, place it in a shaker at 180 r / min, 28° C., and shake the flask for 5 days to obtain the liquid fermentation strain of Trichoderma harzianum SH2303.

[0059] 3. Trichoderma liquid fermentation: corn flour 8kg, magnesium sulfate 80g, ferrous sulfate 1.2g, manganese sulfate 0.4g, zinc sulfate 0.32g, potassium dihydrogen sulfate 0.61kg, sodium nitrate 0.23kg, ammonium nitrate 176g, calcium chloride 160g, make up 200...

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PUM

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Abstract

The invention discloses a trichoderma seed coating as well as a preparation method and an application thereof. The seed coating is prepared from trichoderma chlamydospore fermentation broth, a film-forming agent, chitosan oligosaccharide, phenazino-1-carboxylic acid and humic acid, wherein each liter of trichoderma chlamydospore fermentation broth contains 13%-17%(W / V) of the film-forming agent, 0.35%-0.45%(W / V) of the chitosan oligosaccharide, 0.5%-1.5% mu g / ml of phenazino-1-carboxylic acid and 0.05%-0.1%(W / V) of the humic acid. The seed coating is convenient to prepare, high in efficiency, low in toxicity and relatively safe to environment, performs coating easily and has good control effect on soil-borne diseases of corn.

Description

technical field [0001] The invention relates to a Trichoderma seed coating agent and its preparation method and application, in particular to the preparation method of a bio-control Trichoderma biological seed coating agent and its application technology in the prevention and treatment of corn stem rot, sheath blight and root rot . Background technique [0002] Trichoderma is a ubiquitous habitant fungus in soil and plant rhizosphere ecosystems. Trichoderma is an internationally recognized beneficial microorganism, which mainly controls soil-borne diseases of various crops, including crop seedling disease, root rot and wilt, etc. . Trichoderma not only has the function of preventing and controlling diseases, but also has the functions of promoting plant root growth, improving nutrient utilization efficiency, improving crop yield and stress resistance, and repairing soil. It is a multifunctional microorganism with significant effects on disease prevention and yield increase...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N61/00A01N43/60A01N43/16A01N25/00A01P3/00C12P1/02C12R1/885
CPCA01N25/00A01N43/16A01N43/60A01N61/00A01N63/30C12P1/02C12N1/145C12R2001/885A01N2300/00
Inventor 张婧迪陈捷倪璇王猛孙佳楠余传金高金欣
Owner SHANGHAI JIAO TONG UNIV
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