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Phoebe zhennan tissue culture rapid breeding method

A technology of tissue culture and fast propagation and nanmu, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc. The effect of being conducive to large-scale production

Inactive Publication Date: 2017-02-15
李志勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Phoebe mainly adopts traditional methods such as cutting, grafting and sowing to raise seedlings. There are disadvantages such as high seedling cost, poor growth of seedlings, uneven growth, and failure to fully realize the potential of improved seeds. Meet the needs of the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Collection and disinfection of explants: take as explants the plump shoots of the same year’s sprouting state at the base of Phoebe tree stumps, wash them under running water for 2 hours, soak them in 4% washing powder solution for 6 minutes, and use a soft brush Gently brush the material with 3% washing powder solution, then rinse with tap water in the form of dripping water for 62 minutes, rinse with distilled water for 4 times, soak in 75% ethanol solution for 21 seconds in an ultra-clean workbench, rinse with sterile water for 6 times, and then rinse with 0.02 %Tween-20 with 0.2% mercuric chloride solution for disinfection for 6 minutes, rinsed with sterile water for 4 times, and blotted the water on the surface with sterile filter paper for later use.

[0018] (2) Induction culture: Cut the shoots treated in step (1) into stems of about 2 cm in length and inoculate them into induction medium for cluster bud induction culture. After inoculation, it was placed in...

Embodiment 2

[0023] (1) Collection and disinfection of explants: take as explants the plump shoots of the same year’s sprouting state at the base of Phoebe tree stumps, wash them under running water for 4 hours, soak them in 4% washing powder solution for 9 minutes, and use a soft brush Gently scrub the material with 4% washing powder solution, then rinse with tap water in the form of dripping water for 72 minutes, rinse with distilled water for 5 times, soak in 75% ethanol solution for 32 seconds in an ultra-clean workbench, rinse with sterile water for 6 times, and then rinse with 0.02 % Tween-20 with 0.2% mercuric chloride solution for 8 min, rinsed with sterile water for 6 times, and then blotted the water on the surface with sterile filter paper for later use.

[0024] (2) Induction culture: Cut the shoots treated in step (1) into stems of about 2.5 cm in length and inoculate them into induction medium for cluster bud induction culture. After inoculation, it is illuminated for 15 hour...

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Abstract

The invention discloses a phoebe zhennan tissue culture rapid breeding method. Phoebe zhennan is a main tree species for forestation in the southern China In the prior art, seedling of phoebe zhennan is mainly carried out via conventional methods such as cottage, grafting, and seeding, and defects, such as high seedling cost, poor and nonuniform seedling growth vigour, and poor displaying of fine breed potential capacity, are observed. According to the phoebe zhennan tissue culture rapid breeding method, high quality cloned twigs are taken as explants; explant sterilization, induction culture, clump shoot regeneration, sprouting of adventitious roots, and acclimatization and transplant are carried out so as to obtain phoebe zhennan in vitro regenerated plantlets; the phoebe zhennan tissue culture rapid breeding technical system is established, so that the excellent properties of phoebe zhennan cloning female parent can be maintained, and it is beneficial for large-scale production of phoebe zhennan cloned seedlings.

Description

technical field [0001] The invention relates to the field of nanmu breeding, in particular to a method for rapid propagation of nanmu tissue culture. Background technique [0002] Phoebe grows in the south, mainly in Yunnan, Guizhou, Sichuan, Guangdong, Guangxi, and Hainan. Preferred tree. Its flowers are yellow, clove-like, cyan, and inedible. The trunk is more than ten feet high, the tree diameter is thick, the smell is fragrant, the wood is hard and water-resistant, it is a good material for making pillars and utensils, and it is a good variety of urban greening trees. Phoebe is pungent in nature and flavor, warm in nature, and nontoxic. Functions and indications: cholera vomits continuously, boiled juice and taken. Branches can be used to treat edema, cut nanmu, soak feet with paulownia juice. With the development of the market, the demand for strong seedlings of fine varieties of Phoebe is increasing day by day. Therefore, how to quickly propagate the fine genotype...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02A40/22Y02P60/40
Inventor 李志勇
Owner 李志勇
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