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Method for continuous hydrolysis of cellobiose in straw by utilization of immobilized enzyme microreactor

A technology of microreactor and cellobiose, applied in the direction of enzyme production/bioreactor, immobilized on or in the inorganic carrier, biochemical equipment and methods, etc., to reduce energy consumption, reduce usage, and improve enzymatic hydrolysis efficiency effect

Inactive Publication Date: 2017-02-08
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for continuously hydrolyzing cellobiose in straw by using an immobilized enzyme microreactor to solve the inhibition of the production of cellobiose in the enzymatic hydrolysis process of straw to the entire enzymatic hydrolysis process and realize continuous hydrolysis. chemical production

Method used

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  • Method for continuous hydrolysis of cellobiose in straw by utilization of immobilized enzyme microreactor
  • Method for continuous hydrolysis of cellobiose in straw by utilization of immobilized enzyme microreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Select a 300 cm quartz capillary with an inner diameter of 530 microns. Connect the injection needle with PTFE tube and install it on the injection pump. First, 20% V / V HF aqueous solution was continuously poured into the quartz capillary at a flow rate of 20 μL / min for 30 minutes, washed with purified water until neutral, 1 mol / L hydrochloric acid was continuously poured into the quartz capillary at 30 μL / min for 100 minutes, rinsed with purified water until Neutral, 1mol / L NaOH aqueous solution was continuously poured into the quartz capillary at 30 μL / min for 100 minutes, and washed with purified water until neutral. The treated quartz tube was blown dry with nitrogen gas. A toluene solution of 10% V / V (3-mercaptopropyl)trimethoxysilane was continuously poured into the quartz capillary at a rate of 1 μL / min for 12 hours. Finally, absolute ethanol was continuously poured into the quartz capillary at a rate of 20 μL / min for 100 minutes, and dried.

[0028] The enzym...

Embodiment 2

[0032] Select a 300 cm quartz capillary with an inner diameter of 530 microns. Connect the injection needle with PTFE tube and install it on the injection pump. First, 20% V / V HF aqueous solution was continuously poured into the quartz capillary at a flow rate of 20 μL / min for 30 minutes, washed with purified water until neutral, 1 mol / L hydrochloric acid was continuously poured into the quartz capillary at 30 μL / min for 100 minutes, rinsed with purified water until Neutral, 1mol / L NaOH aqueous solution was continuously poured into the quartz capillary at 30 μL / min for 100 minutes, and washed with purified water until neutral. The treated quartz tube was blown dry with nitrogen gas. A 10% V / V toluene solution of (3-mercaptopropyl)trimethoxysilane was continuously poured into the quartz capillary at a rate of 1 μL / min for 8 hours. Finally, absolute ethanol was continuously poured into the quartz capillary at a rate of 20 μL / min for 100 minutes, and dried.

[0033] The enzyme...

Embodiment 3

[0037] Select a 300 cm quartz capillary with an inner diameter of 530 microns. Connect the injection needle with PTFE tube and install it on the injection pump. First, 20% V / V HF aqueous solution was continuously poured into the quartz capillary at a flow rate of 20 μL / min for 30 minutes, washed with purified water until neutral, 1 mol / L hydrochloric acid was continuously poured into the quartz capillary at 30 μL / min for 100 minutes, rinsed with purified water until Neutral, 1mol / L NaOH aqueous solution was continuously poured into the quartz capillary at 30 μL / min for 100 minutes, and washed with purified water until neutral. The treated quartz tube was blown dry with nitrogen gas. A toluene solution of 10% V / V (3-mercaptopropyl)trimethoxysilane was continuously poured into the quartz capillary at a rate of 1 μL / min for 10 hours. Finally, absolute ethanol was continuously poured into the quartz capillary at a rate of 20 μL / min for 100 minutes, and dried.

[0038] The enzym...

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Abstract

The invention discloses a method for continuous hydrolysis of cellobiose in straw by the utilization of an immobilized enzyme microreactor. The method is characterized in that beta-glucosidase is immobilized in a microreactor and a substrate cellobiose aqueous solution is injected into the microreactor to carry out a micro reaction so as to degrade the cellobiose solution into glucose, wherein a carrier for enzyme immobilization is a quartz capillary tube and a coupling agent for enzyme immobilization is a toluene solution of (3-mercaptopropyl)trimethoxy silane. By the utilization of the microreactor, cellobiose in straw undergoes enzymatic hydrolysis to generate monosaccharide. The enzyme dosage is low, reaction efficiency is greatly raised, and energy consumption is reduced. By changing operation condition control, hydrolysis rate of cellobiose can be remarkably raised, and handling capability of straw is greatly enhanced. The device is a capillary holow tube, and the structure is simple. Only by simply amplifying number, production capacity can be increased proportionally. The method of the invention has a good industrial prospect.

Description

technical field [0001] The invention relates to the field of biocatalysis, in particular to a method for continuously hydrolyzing cellobiose in straw by using an enzyme microreactor. Background technique [0002] As a large agricultural country, our country can generate more than 700 million tons of straw every year, which has become "waste" that is "not very useful" but must be disposed of. If it is completely handled by individuals, there will be a large amount of burning, which will not only waste resources but also pollute the environment. If straw is combined with modern biotechnology, its utilization rate can be greatly improved. Crop straw is mainly composed of cellulose, hemicellulose and lignin. Although the content of lignin is not high, it is wrapped on the outside of cellulose and has a strong chemical connection with cellulose and hemicellulose, which hinders the degradation of cellulose and hemicellulose, thus greatly affecting the degradation rate of straw ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12N9/42C12N11/14C12P19/02C12P19/14
CPCC12M21/18C12N9/2445C12N11/14C12P19/02C12P19/14C12Y302/01021
Inventor 韦策周艳张红漫
Owner NANJING UNIV OF TECH
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