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Application of Lithocarpus polystachyus (Wall.) Rehd. in preparing toothpaste

A technology of polychthonium and toothpaste, which is applied in the application field of polychthonium in the preparation of toothpaste, to achieve the effect of promoting cell proliferation

Inactive Publication Date: 2017-02-08
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, it has not been reported that the extract of Glycyrrhizae polycarp is used in toothpaste

Method used

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  • Application of Lithocarpus polystachyus (Wall.) Rehd. in preparing toothpaste
  • Application of Lithocarpus polystachyus (Wall.) Rehd. in preparing toothpaste
  • Application of Lithocarpus polystachyus (Wall.) Rehd. in preparing toothpaste

Examples

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example 1

[0019] (1) Cell lines, main reagents and instruments

[0020] Human gingival fibroblasts (HGF-1) were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA). Inoculated in DMEM / F-12 medium containing 10% fetal bovine serum, 2% glutamine, at 37°C, 5% CO 2 cultured in an incubator. DMEM / F-12 culture medium was purchased from Hyclone Company, methylthiazolium blue MTT, M2128), prostaglandin E2 (PGE 2 201508) kits were purchased from Shanghai Jianglai Biotechnology Co., Ltd. COX-1 (ab695) and COX-2 (ab52237), and antibodies were purchased from Abcam. DDY-10 three constant electrophoresis instrument (Bio-Rad, USA), semi-dry transfer tank (Bio-Rad, USA), automatic electrophoresis gel imaging analysis system (Bio-Rad, USA).

[0021] (2) Experimental method

[0022] 1) The effect of the extract of Glycyrrhizae on the growth of HGF-1 cells Take the HGF-1 cells in the logarithmic growth phase, and dilute 1×10 5 cells / ml were inoculated in 96-well plates, and af...

example 2

[0042] A kind of toothpaste containing the extract of Rhododendron, which is made of the following components by weight percentage: 50% silicon dioxide, 15% glycerin, 15% extract from Rhododendron, 2% carrageenan, 12% Sodium alkyl sulfate 4%, essence 1.5%, preservative 2%, deionized water balance. The essence is peppermint essence; the preservative is sodium benzoate; and since the polyspinae itself contains phloridzin and has a sweet taste, no sweetener is needed in the formula.

[0043] The above-mentioned preparation method of a toothpaste containing the extract of Saccharomyces radicis comprises the following steps:

[0044] (1) Take the raw material of Duosuishike, wash it, add water 10 times the volume of the raw material of Duosuishike, and decoct for the first time. The decoction time is 2 hours, and then add water until the water volume is 8 times the volume of Duosuishike. Double decocting for the second time, the decoction time is 1 hour, to obtain the decoction of...

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Abstract

The invention discloses a toothpaste containing a Lithocarpus polystachyus (Wall.) Rehd. extract. The toothpaste is prepared from the Lithocarpus polystachyus (Wall.) Rehd. extract, silicon dioxide, carageenan, lauryl sodium sulfate, glycerol and other auxiliary ingredients. The toothpaste has the beneficial effects that by use of the anti-inflammation efficacy and gingival atrophy resistant efficacy of a traditional Chinese medicine Lithocarpus polystachyus (Wall.) Rehd., the Lithocarpus polystachyus (Wall.) Rehd. is compounded with the other auxiliary ingredients so as to obtain the toothpaste capable of resisting inflammation and preventing gingival atrophy, and after being used for a long term, and the toothpaste is capable of enhancing the inflammation resisting capability of a human body and achieving the effects of preventing periodontal inflammation and gingival atrophy.

Description

technical field [0001] The present invention relates to the application of polyspinae in the preparation of toothpaste. Background technique [0002] Periodontitis is a group of diseases mediated by bacteria that lead to inflammatory destruction of the tissues surrounding the teeth. Gingival fibroblasts (human gingival fibroblast, HGF) are the main constituent cells of gingival connective tissue, directly contact and react with pathogenic bacteria and their products, and play an important role in the occurrence and development of periodontal inflammation. Lipopolysaccharide (LPS), one of the main virulence factors of Gram-negative anaerobic bacilli, can induce inflammation and death of HGF cells, and is the main pathogen of periodontal disease. Therefore, inhibiting gingival cell inflammation caused by LPS is an important measure to prevent periodontitis. Lithocarpus Polystachys (Wall.) Rehd is a plant that has a long history of use in my country and has the same origin as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q11/00A61P1/02
CPCA61K8/97A61K36/185A61Q11/00
Inventor 龚其海高健美徐应淑
Owner ZUNYI MEDICAL UNIVERSITY
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