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Kit for jointly detecting and evaluating liver cancer transfer and recurrence risks after orthotopic liver transplantation

A detection reagent and liver transplantation technology, applied in the field of medical biological detection, to prevent disease progression and improve prognosis

Active Publication Date: 2017-02-01
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the expression rate of EpCAM in HCC is only 14.3-39%.

Method used

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  • Kit for jointly detecting and evaluating liver cancer transfer and recurrence risks after orthotopic liver transplantation
  • Kit for jointly detecting and evaluating liver cancer transfer and recurrence risks after orthotopic liver transplantation
  • Kit for jointly detecting and evaluating liver cancer transfer and recurrence risks after orthotopic liver transplantation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: preparation kit of the present invention

[0057] The kit of the present invention consists of the following:

[0058] A) AFP detection contains reagents

[0059] 1) R1:Access AFP kit: 6 boxes, 50 tests / box.

[0060] 2) R1a: Magnetic particles coated with rat anti-AFP monoclonal antibody are suspended in TRIS buffer saline containing ionic surfactant, bovine serum albumin (BAS), <0.1% sodium azide and 0.1% ProClin300.

[0061] 3) R1b: Rat anti-AFP monoclonal antibody enzyme conjugate labeled with alkaline phosphatase (calf) diluted in phosphate buffered saline, containing ionic surfactant, bovine serum albumin (BSA), protein (sheep sex, rabbit, mouse), <0.1% sodium azide and 0.25% ProClin300.

[0062] 4) S0~S6: Access AFP calibrator: S0: buffer solution based on bovine serum albumin (BSA), also contains ionic surfactant, <0.1% sodium azide and 0.1% ProClin300, the concentration of AFP contained 0.0ng / ml.

[0063] S1, S2, S3, S4, S5, S6: The AFP concen...

Embodiment 2

[0083] Embodiment 2: the detection method of the kit of embodiment 1

[0084] 1. Blood sample collection and sample preparation:

[0085] Due to the advantages of convenient, non-invasive, and continuous detection of peripheral blood, the detection of biomarkers of liver cancer metastasis from serum can detect liver cancer metastasis early and improve the diagnosis of liver cancer to a new level.

[0086] Blood samples were collected in Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine: 30 liver transplant patients with liver cancer and 18 liver transplant patients without liver cancer. Sample collection: Collect about 3-6ml of peripheral blood from the patient and put it into a vacuum coagulation tube, invert it several times to fully combine, let it stand for about half an hour, centrifuge at 1600g for 10min, and transfer the supernatant to a new tube. The supernatant was centrifuged at 12000 g for 10 min for the second time, and the supernatant w...

Embodiment 3

[0112] Example 3: ROC model analysis of AFP content and CTC number after liver transplantation for primary liver cancer

[0113] The present invention monitors the AFP content and the number of CTCs in the serum of 30 patients with liver cancer before and after liver transplantation, and through ROC analysis and rank sum test, it is found that the combined diagnosis of AFP and iFISH has a significant effect in the first 3 months of recurrence AUC 联合复发前 =0.831, PAFP =0.643, P>0.05; AUC iFISH =0.643, P>0.05( figure 1 ). Although the specificity of AFP detection alone reaches 95.2%, its sensitivity is only 33.3%. The sensitivity and specificity of iFISH detection alone were 66.7% and 76.2%, respectively. The combined detection of AFP+iFISH can increase the sensitivity to 88.9%, and the specificity still maintains a high level, the joint diagnosis point P=0.5224 (Table 1). The present invention discovers for the first time that the combined diagnosis of AFP and iFISH can predi...

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Abstract

The invention relates to the field of medical biological detection, in particular to a detection reagent or a kit for evaluating liver cancer transfer and recurrence risks after orthotopic liver transplantation through combination of AFP (alpha fetoprotein) detection and iFISH-CTC (circulating tumor cells) detection, and a detection method of the detection reagent or the kit. The liver cancer transfer and recurrence risks after the orthotopic liver transplantation are evaluated through detection of the content of AFP and the number of CTC, so that a new measure is provided for detection of liver cancer transfer and recurrence after the orthotopic liver transplantation so as to start clinical intervention as early as possible, worsening of patients' conditions is prevented, and prognosis of patients is improved.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to a detection kit and a detection method for evaluating the risk of metastasis and recurrence of liver cancer after liver transplantation by combining AFP and iFISH-CTC detection. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world, and its morbidity and mortality rank fifth and third among all tumor diseases (Zhang Y, Li J, Cao L, et al. Circulating tumor cells in hepatocellular carcinoma: detection techniques, clinical implications, and future perspectives[J].Semin Oncol,2012,39(4):449-460.). my country is a country with a large number of liver diseases. About 110,000 people die from HCC every year, accounting for 45% of the global liver cancer deaths. Liver transplantation is one of the important methods for the treatment of liver cancer. Liver cancer patients account for about 40-50% of the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C12Q1/02
CPCG01N33/5044G01N33/68G01N2333/47G01N2800/245
Inventor 薛峰夏强顾健人张桢珍施少军
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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