Neuronal cell culture solution and neuronal cell culture method

Abstract

The invention discloses a culture solution for culturing neuronal cells based on peripheral blood mononuclear cells. The culture solution comprises a DMEM / F12 basic culture medium and a nerve cell culture medium which have a volume ratio of 1 to (0.8 to 1.2), nerve growth factor, an adenylate cyclase activator, a serum substitute, L-ascorbic acid, L-glutamine derivatives, an ALK inhibitor, a GSK-3 beta inhibitor, a GSK 3 alpha inhibitor, an ALK-2, 3, 6, AMPK inhibitor and a TGF-beta R inhibitor. The invention also discloses a method for culturing the neuronal cells based on the peripheral blood mononuclear cells. The culture solution can be used for carrying out directional differentiation culture by taking the peripheral blood mononuclear cells as a raw material so as to obtain the neuronal cells. The culture solution is convenient in material obtaining and free from quantitative limitation, and can avoid moral controversy.

Description

technical field [0001] The invention belongs to the technical field of neuron cell culture, and in particular relates to a culture medium and a culture method of neuron cells. Background technique [0002] Nervous system diseases, especially central nervous system degenerative diseases are common and frequently-occurring diseases in clinical practice, which seriously endanger human health. According to a report released by the World Health Organization in February 2007, more than 1 billion people worldwide suffer from neurodegenerative diseases. In 2005, more than 24 million people worldwide suffered from Alzheimer's disease; other degenerative diseases of the central nervous system, Diseases such as multiple sclerosis, Huntington's disease, and Parkinson's disease also cause great suffering to patients. [0003] Studies have shown that the use of human neuron cells can be used to study the mechanism of nerve damage and screen for new drugs that interfere with nerve functio...

AI Technical Summary

Problems solved by technology

However, the preparation cycle of induced pluripotent stem cells is long, generally takes 4 to 8 months, and after obtaining induced pluripotent stem cells, it takes 1 to 2 months to differentiate to obtain neuron cells, which makes the acquisition cycle of neuron cells the shortest It takes about half a year, and the long cycle will inevitably make the preparation cost higher

In addition, the cell stability obtained by this method is relatively lacking, which has the risk of carcinogenesis

[0006] In summary, the induction of differentiation from embryonic stem cells is subject to ethical and moral constraints, the number of neuronal cells obtained is limited and has immune rejection, and the preparation cycle of induction of differentiation from induced pluripotent stem cells is long and costly, which makes neuronal cells in disease mechanisms Applications in research, drug screening, and clinical cell therapy are largely limited

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