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Application of potato whole powder in preparing PDA (potato dextrose agar) medium

A technology of potato whole powder and culture medium, applied in the direction of cultivation process, tissue culture, biochemical equipment and methods, etc., can solve the problems of troublesome preparation of PDA medium, waste of potato nutrients, high price, etc. Germination, shortened preparation time, wide-ranging effects

Pending Publication Date: 2017-02-01
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the disadvantages of existing PDA culture medium, such as troublesome preparation, waste of potato nutrients, high cost, high price, etc., the purpose of the present invention is to provide the application of whole potato powder in the preparation of PDA culture medium

Method used

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  • Application of potato whole powder in preparing PDA (potato dextrose agar) medium
  • Application of potato whole powder in preparing PDA (potato dextrose agar) medium
  • Application of potato whole powder in preparing PDA (potato dextrose agar) medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] The preparation of the potato whole powder medium of embodiment 1 different glucose content

[0106] Prepare as follows:

[0107] Table 1 Composition and ratio of potato whole powder medium with different glucose contents

[0108]

[0109] Weigh potato whole powder (produced by Xinghua Lianfu Food Co., Ltd.), glucose and agar powder according to Table 1, mix well, then add into 3 different 1000ml triangular flasks, add 800ml water, shake well, Cover with parafilm or kraft paper and sterilize at 121°C for 30min. When it is not solidified, pour it into a petri dish, pour 20-25ml of culture medium into each petri dish with a diameter of 9cm, and set it for use.

Embodiment 2

[0110] The preparation of the potato whole flour medium of embodiment 2 different potato whole flour content

[0111] Prepare as follows:

[0112] Weigh potato whole powder (produced by Xinghua Lianfu Food Co., Ltd.), glucose and agar powder as shown in Table 2, mix well, then add into 3 different 1000ml triangular flasks, add 800ml water, shake well, Cover with parafilm or kraft paper and sterilize at 121°C for 30min. When it is not solidified, pour the petri dish, pour 20-25ml of medium into each petri dish with a diameter of 9cm, and set it for use.

[0113]Table 2 Composition and ratio of potato whole flour medium with different content of potato whole flour

[0114]

Embodiment 3

[0115] Example 3 at Difco TM Contrastive Experiment of Edible Fungus Cultivation on PDA Medium and Potato Whole Powder Medium

[0116] (1) Test materials:

[0117] 1. Culture medium:

[0118] (1) Potato whole powder medium prepared in Example 1 (numbered as A, B and C).

[0119] (2) Difco TM PDA medium, prepared according to the operating instructions.

[0120] (3) Ordinary PDA medium, prepared according to conventional methods.

[0121] 2. Edible mushroom strains: oyster mushroom CCEF89, shiitake mushroom 808, black fungus black 29, hair fungus sumo No. 3, Agaricus bisporus W192, enoki mushroom jinza 19, king oyster mushroom 3898, Bailing mushroom Zhongnong No. 1, slip mushroom early birth 2 and other 9 edible fungus strains. All strains were obtained from the Institute of Agricultural Resources and Agricultural Zoning, Chinese Academy of Agricultural Sciences.

[0122] (2) Test method:

[0123] 9 kinds of oyster mushroom CCEF89, shiitake mushroom 808, black fungus ...

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Abstract

The invention discloses an application of potato whole powder in preparing a PDA (potato dextrose agar) medium, and belongs to the field of microbial culture mediums. The invention also discloses a potato whole powder medium, wherein the potato whole powder medium consists of the following components according to proportions: 10-20g of the potato whole powder, 2-10g of glucose, 12-20g of agar powder and 1000ml of water. The invention also discloses an application of the potato whole powder medium in fungal culture. According to the potato whole powder medium provided by the invention, such nutrient substances as starch, protein, vitamins, mineral substances and the like in potatoes are completely reserved, so that comprehensive nutrition is guaranteed, and edible fungi, on the potato whole powder medium, are high in mycelia growth speed and vigorous in growth; moreover, the potato whole powder medium provided by the invention is simple in preparation method and the preparation method is free from such processes as preparing potato juice and the like, so that a preparation time is greatly shortened; in addition, the potato whole powder medium provided by the invention is low in production cost; and the potato whole powder medium provided by the invention, since all ingredients are in the form of dry powder, are quite convenient to store and transport.

Description

technical field [0001] The invention belongs to the field of microbial culture medium, and in particular relates to the application of whole potato powder in the preparation of PDA culture medium; it also relates to a whole potato flour culture medium, a preparation method and application of the culture medium. Background technique [0002] PDA medium (abbreviation of Potato Dextrose Agar (Medium)) is a commonly used medium, which is often used to cultivate yeast, mold, edible fungus and other fungi. PDA medium has good versatility and can meet the requirements of absolute The basic growth requirements of most edible fungi, but because the potato juice must be prepared first in the preparation process, that is, fresh potatoes without mildew and sprouts, washed and peeled, cut into small pieces, boiled in water, filtered with gauze, Prepare potato juice; this juice extraction process not only makes the preparation process of PDA medium very cumbersome and time-consuming, but ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/16
CPCC12N1/14C12N1/16C12N2500/76
Inventor 陈强管建华黄晨阳张金霞
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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