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Application of LOC80054 in diagnosis or prognosis of pancreatic cancer

A technology for pancreatic cancer and prognosis, applied in the field of molecular markers for detecting pancreatic cancer, can solve the problems of occult onset, difficulty in early diagnosis, and lack of typical clinical symptoms.

Inactive Publication Date: 2017-01-25
叶伟亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the treatment of pancreatic cancer, early diagnosis is difficult, its onset is hidden, lack of typical clinical symptoms, and its prognosis is extremely poor. Pancreatic cancer is highly aggressive and malignant, and the curative effects of surgery, chemotherapy and radiotherapy are not satisfactory. , the 1-year survival rate after surgery is less than 20%, and the 5-year survival rate is only 4%. The low early diagnosis rate and postoperative metastasis are the main reasons for the high mortality rate of pancreatic cancer
So far, no relevant research reports on the role of LOC80054 in pancreatic cancer have been found

Method used

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  • Application of LOC80054 in diagnosis or prognosis of pancreatic cancer
  • Application of LOC80054 in diagnosis or prognosis of pancreatic cancer
  • Application of LOC80054 in diagnosis or prognosis of pancreatic cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 实施例1基于TCGA数据库信息进行胰腺癌的生存分析筛选相关基因

[0027] 1、临床信息筛选

[0028] 检索TCGA数据库中的胰腺癌患者临床信息,截至2015年12月10日,TCGA数据库中总共记载了185例胰腺癌临床病例。对这些数据进行筛选,共有160例患者纳入研究。筛选时排除具有其他恶性肿瘤病史、曾接受过放疗或化疗的患者,同时纳入研究的患者需含临床信息和mRNA数据。2、生存期研究样本统计

[0029] 160例胰腺癌患者生存时间的统计结果如下表所示:

[0030] 表1 160例胰腺癌患者生存时间统计

[0031] Survival time t (years) The number of people who entered the research at the beginning of the period Number of deaths during the period Number of people lost to follow-up during the period t<1

160 27 74 1≤t<2

59 22 15 2≤t<3

22 10 2 3≤t<4

10 2 2 4≤t<5

6 0. 1 5≤t 5 4 1

[0032] 3、mRNA表达数据生存分析研究方案

[0033] (1)对胰腺癌高通量mRNA转录组数据检索、数据下载及样本挑选和归类。由下载的转录组数据通过生物信息学分析筛选得出与胰腺癌生存时间相关的mRNA。

[0034] (2)用Cytoscape软件构建由mRNAs组成的生物信息网络,通过DAVID对生物信息网络中与胰腺癌生存时间相关的mRNA进行GO分析和Pathway分析。

[0035] 4、胰腺癌mRNA表达数据的生存分析结果

[0036] 将胰腺癌组织的转录组数据下载后,去除read count=0小于20%的mRNA后用做下一步分析,包括17100个mRNA。提取mRNA基因表达量和胰腺癌TCGA数据库生存时间数据,采用survival包的coxph函数完成,经单因素Cox回...

Embodiment 2

[0038] 实施例2RT-PCR检测LOC80054在胰腺癌和癌旁组织中的表达

[0039] 1、材料

[0040] 1.1组织病理标本

[0041]Tissues from 160 patients with pancreatic cancer who underwent surgical resection and were confirmed by pathological examination and corresponding paracancerous tissue samples were collected. Paracancerous tissue was defined as pancreatic tissue beyond 3 cm from the tumor edge. The samples were collected from patients who were diagnosed with pancreatic cancer and underwent surgical resection from October 2012 to December 2015 at Peking Union Medical College Hospital. Immediately after surgical resection of pancreatic cancer tissue, under the guidance of pathologists, pancreatic cancer and paracancerous tissues were taken and placed in liquid nitrogen, and stored in a -80°C low-temperature refrigerator after serial numbering.

[0042] 1.2 The inclusion criteria are as follows:

[0043] (1) Cases undergoing surgical resection;

[0044] (2) Pancreatic cancer was diagnosed pathologically after su...

Embodiment 3

[0081] The preparation of embodiment 3 kits

[0082] Based on the primer set obtained in Example 2, the detection kit of LOC80054 of the present invention is assembled, and the kit includes a primer pair for specific amplification of LOC80054 as shown in SEQ ID NO: 2 and SEQ ID NO: 3, and a specific amplification primer The primer pair for the augmentation housekeeping gene (GAPDH) is shown in SEQ ID NO: 4 and SEQ ID NO: 5; it also includes a SYBR Green polymerase chain reaction system, such as PCR buffer, SYBR Green fluorescent dye, and dNTPs. The composition of described PCR buffer solution is 25mM KCl, 2.5mMMgCl 2 , 200mM (NH 4 ) 2 SO 4 ; Also includes normal pancreas tissue cDNA: used as a negative control and detection sample cDNA for quantitative PCR detection, each reaction system uses the same amount as the detection sample cDNA.

[0083] Through the optimization of primer concentration and annealing temperature, the reaction system was finally determined as shown ...

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Abstract

The invention discloses application of LOC80054 in diagnosis or prognosis of pancreatic cancer. The LOC80054 serves as a molecular marker. The invention further discloses the application of LOC80054 in preparing a diagnosis or prognosis kit for pancreatic cancer. The diagnosis or prognosis means diagnosis, therapeutic effect evaluation or metastatic recurrence monitoring. The molecular marker is utilized to screen, diagnose, treat, monitor and pre-diagnose the pancreatic cancer high risk group. LOC80054 has significance in diagnosing and treating pancreatic cancer.

Description

technical field [0001] 本发明涉及生物检测领域,具体涉及利用与胰腺癌相关的lncRNA LOC80054作为检测胰腺癌的分子标记物及其用途。 Background technique [0002] 胰腺癌是一种消化道常见肿瘤,是预后最差的恶性肿瘤之一。根据最新流行病学调查,胰腺癌位居欧美等发达国家恶性肿瘤死亡率第四位,全球每年约250万人死于胰腺癌。近20年来我国胰腺癌的发病率持续增高,目前胰腺癌位居恶性肿瘤死亡率第五位。在胰腺癌的治疗中,早期诊断困难,其起病隐匿,缺乏典型临床症状,且预后极差,胰腺癌侵袭性强,恶性度高,外科手术、化疗及放疗等手段的疗效均不尽人意,其术后1年生存率不到20%,5年生存率仅为4%,早期确诊率低和术后转移是胰腺癌死亡率高的主要原因。因此,胰腺癌的早期诊断和早期治疗是提高和改善胰腺癌预后的关键,尤其是发现一种特有的、可用于早期诊断和预后的标记物和靶向分子,对于战胜胰腺癌具有重要意义,成为目前国内外肿瘤专家的研究热点。 [0003] 近年来一些研究表明非编码RNA(ncRNAs)是一类具有重要生物学功能的RNA,参与基因组印记、染色体沉默、染色质修饰、转录激活、转录干扰、核内运输等多种重要的调控过程,在细胞分化和发育、基因转录和翻译、遗传和表观遗传等生命活动中均发挥重要的调控作用。越来越多的权威研究证实lncRNA在肿瘤的发生发展中起着抑制或促进肿瘤的作用,在调控肿瘤细胞增殖、凋亡、细胞周期、侵袭转移能力等方面,均具有十分重要作用。目前己有较多lncRNAs被证实在包括乳腺癌、前列腺、黑色素瘤、肝癌、结肠癌、膀胱癌等在内的人类多种肿瘤中存在差异表达并执行重要的调控功能。目前尚未发现关于LOC80054在胰腺癌中作用的相关研究报道。 [0004] 由于胰腺癌发病隐匿、进展快且预后极差,本领域仍需一种准确、灵敏、特异性强的标记物用于辅助胰腺癌的诊断或预后,因此新的胰腺癌标记物的开发成为迫切的需求。 Contents of the invention [0005] 为了实现胰腺癌的早期发现,早期干预,本发明的目的在于提供一种新的与胰腺癌相关的分子标记物。 [0006] 本发明的还一目的是提供LOC80054在胰腺癌诊断或预后中的用途。 [0007] 为实现上述目的,本发明...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 叶伟亮吴志宏邱宾涛
Owner 叶伟亮
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