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Application of SNHG10 (small nucleolar RNA host gene 10) in preparation of preparations for diagnosing prostate cancer

A prostate cancer and preparation technology, applied in the field of biomedicine, can solve problems affecting the treatment effect of tumors

Inactive Publication Date: 2017-01-25
北京致成生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemoradiotherapy drugs not only act on the tumor, but can also act on the healthy tissues around the tumor. Therefore, while killing the tumor, they also bring great side effects to the body, which ultimately affects the therapeutic effect on the tumor.

Method used

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  • Application of SNHG10 (small nucleolar RNA host gene 10) in preparation of preparations for diagnosing prostate cancer
  • Application of SNHG10 (small nucleolar RNA host gene 10) in preparation of preparations for diagnosing prostate cancer
  • Application of SNHG10 (small nucleolar RNA host gene 10) in preparation of preparations for diagnosing prostate cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 High-throughput sequencing screening of differentially expressed genes

[0031] 1. Sampling

[0032] From October 2012 to December 2015, 27 tissue samples were obtained from the Department of Urology at Peking Union Medical College Hospital. All samples were confirmed by pathological examination, including 8 cases of paracancerous tissue samples and 19 cases of prostate cancer samples. Store in -80°C low temperature refrigerator.

[0033] 2. Total RNA extraction from tissue samples

[0034] use Reagent (invitrogen, Cat. No. 15596-018) was used to extract sample RNA, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0035] After collecting the samples, freeze them in liquid nitrogen. After taking them out, put the tissue into a pre-cooled mortar for grinding. After the tissue sample is powdered:

[0036] ① Add Trizol and store at room temperature for 5 minutes;

[0037] ② Add 0.2 mL of...

Embodiment 2

[0046] Example 2 RT-PCR verification of expression of SNHG10 in prostate cancer tissue and adjacent tissue

[0047] 1. Materials

[0048] 20 prostate cancer tissue samples and 8 paracancerous tissue samples were collected from prostate cancer samples during urological surgery in Peking Union Medical College Hospital from 2012 to 2015, and they were grouped and numbered. All samples were confirmed by pathological examination.

[0049] 2. Method

[0050] 2.1 Extract the total RNA from the tissue sample, the same as the extraction method in Example 1.

[0051] 2.2 Synthesis of cDNA by reverse transcription

[0052] use III Reverse Transcriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse transcription, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0053] Using a reverse transcription kit, 1 μg of total RNA was reverse-transcribed with reverse transcription buffer to synthesize cDNA. A...

Embodiment 3

[0067] Example 3 Gene chip verification of SNHG10 expression in prostate cancer tissue and paracancerous tissue samples

[0068] 1, the acquisition of material, with embodiment 2.

[0069] 2, the extraction of total RNA, with the method for embodiment 1.

[0070] 3. Gene chip verification

[0071] After the total RNA was linearized and amplified, the cy3-UTP labeled and fluorescently labeled cRNAs were purified using the RNEASY Mini Kit, and the labeled cRNAs were fragmented with Amhion’s RNA Fragmentation Reagents. Human whole gene expression profile chips (4x 44K genes) from Agilent Company of the United States were used, hybridized in a chip hybridization oven at 65°C for 17 hours, then eluted, stained, and finally scanned with an Agilent DNA MicroarrayScanner scanner.

[0072] After the hybridized chip reads the data points by the chip scanner, the data is imported into the analysis software, and the absolute value of the natural logarithm of the ratio of the two groups ...

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Abstract

The invention discloses application of SNHG10 (small nucleolar RNA host gene 10) in the preparation of preparations for diagnosing prostate cancer. A preparation for diagnosing prostate cancer comprises detecting expression level of SNHG10 by using fluorescent quantitative PCR (polymerase chain reaction) method or a gene chip. The invention also discloses a kit for diagnosing prostate cancer, and the kit can detect the expression level of nucleotide sequence, as shown by SEQ ID NO: 1, in a biological sample in order to diagnose prostate cancer. The invention discloses SNHG10 associated with prostate cancer and further verifies that SNHG10 has expression downregulation in prostate cancer. By using SNHG10 to detect prostate cancer, it is possible to quickly and effectively achieve early detection, and treatment targets and important basis are provided for clinical applications such as gene therapy and drug therapy.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of human SNHG10 in the preparation of diagnostic preparations for prostate cancer. Background technique [0002] Prostate cancer is one of the common male reproductive system malignancies. Worldwide, the incidence of prostate cancer ranks second among all malignant tumors in men. In the United States, the incidence of prostate cancer has surpassed that of lung cancer, becoming the first tumor that endangers men's health. The incidence of prostate cancer in Asia is far lower than that in European and American countries, but it has shown an upward trend in recent years, and the growth rate is faster than that in developed countries in Europe and America. Prostate cancer patients are mainly elderly men, usually after the age of 50, 95% occur in elderly men over 60 years old, and the incidence rate continues to increase with age. Prostate cancer often has no symptoms in t...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00C12Q2600/158C12Q2600/178
Inventor 刘昊
Owner 北京致成生物医学科技有限公司
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