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Extract from active parts of okra for anti-fatigue and its preparation method and application

An effective part and anti-fatigue technology, which is applied to the extract of okra anti-fatigue effective part and its preparation and application fields, can solve the problems of restricting popularization and application, unclear anti-fatigue active parts and active components of okra, etc.

Active Publication Date: 2019-08-09
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the anti-fatigue effect of okra has been reported in the literature, so far, the anti-fatigue active parts and active components of okra are still unclear, which seriously restricts its popularization and application in the preparation of medicines, health products or food

Method used

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  • Extract from active parts of okra for anti-fatigue and its preparation method and application
  • Extract from active parts of okra for anti-fatigue and its preparation method and application
  • Extract from active parts of okra for anti-fatigue and its preparation method and application

Examples

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specific Embodiment approach

[0038] The present invention will be described below through specific examples, but the present invention is not limited thereto. The methods described in the following examples are conventional methods unless otherwise specified, and the reagents and biological materials can be obtained from commercial sources unless otherwise specified.

[0039] The reference substance gallic acid used in the present invention was purchased from China Institute for the Control of Pharmaceutical and Biological Products, and the batch numbers were 110831-201204 respectively. Quercetin-3-O-gentiobioside and isoquercitrin were purchased from Chengdu Pufeid Biotechnology Co., Ltd.

Embodiment 1

[0040] The preparation of embodiment 1 okra effective fraction extract

[0041] 1. Take tender okra fruit, weigh 2000g fresh tender fruit (equivalent to 200g dried fruit), add distilled water 2000ml, and reflux extraction for 60 minutes;

[0042] 2. Pour out the extract, repeat the reflux extraction twice according to the extraction conditions and method for the first time, and combine the extract for three times;

[0043] 3 When the extract is concentrated under reduced pressure to 2000ml, use D101 resin (purchased by Hebei Cangzhou Baoen Chemical Co., Ltd.) to absorb, elute with distilled water, and collect the distilled water eluate; then elute with 90% ethanol, collect the ethanol eluate ;

[0044] 4 Concentrating and drying the distilled water eluate to obtain the okra water-eluted extract (HQKS1); concentrating and drying the ethanol eluate to obtain the anti-fatigue effective part extract (HQK1).

Embodiment 2

[0045] The preparation of embodiment 2 okra effective parts extract

[0046] Okra water extract adopts DM130 macroporous resin (Hebei Cangzhou Baoen Chemical Co., Ltd. purchases) to absorb, and other methods are exactly the same as Example 1 to obtain okra water-eluted extract (HQKS2) and anti-fatigue effective part extract (HQK2) .

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Abstract

The invention discloses an extract of an active anti-fatigue part of okra, and a preparation method and application thereof. The extract of the active anti-fatigue part of okra uses total polyphenols as a main active anti-fatigue component, wherein the total polyphenols account for 11.79 to 23.34% of the total mass of the extract of active anti-fatigue part and mainly comprise catechin derivatives and quercetin derivatives, and the quercetin derivatives are mainly composed of quercetin-3-O-gentiobioside, quercetin-3-O-glucose-xyloside, isoquercitrin, quercetin-3-O-malonyl-glucoside, rutin and quercetin. The extract of the active anti-fatigue part of okra provided by the invention can substantially prolong the load swimming time of mice, eliminate lactic acid accumulation after sports, reduce the content of serum urea nitrogen, increase hepatic glycogen and improve the antioxidation capability of mice in a stress state, and has substantial anti-fatigue effect.

Description

technical field [0001] The invention relates to the anti-fatigue effective part extract of okra and a preparation method thereof, and also relates to the use of the okra effective part extract in preparing anti-fatigue medicine, health care product or food. Background technique [0002] Fatigue is a complex physiological and biochemical change process of the body, and it is a normal physiological phenomenon that must occur when human mental or physical strength reaches a certain stage. It not only marks the temporary decline of the original working ability of the body, but also may be a precursor to the development of the body into a state of injury. According to the WHO survey, more than 35% of people in the world are in a state of fatigue, and the fatigue state of middle-aged male population reaches more than 60%. The appearance of fatigue can lead to reduced athletic ability, reduced combat effectiveness, reduced work efficiency, and increased accidents. If fatigue is no...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K31/353C07H17/07C07H1/08A61K36/185A61P39/00A23L33/105
Inventor 潘瑞乐刘新民夏方博
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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