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Nano-fibrosis silk fibroin gel and preparation method thereof

A technology of silk fibroin and nanofibers, applied in medical science, prostheses, etc., can solve the problems of not having a bionic nanofiber structure, affecting the mechanical properties of silk fibroin hydrogel, and the complicated preparation process of silk fibroin hydrogel , to achieve the effects of strong maneuverability, avoiding the toxicity of the stent, and excellent mechanical properties

Active Publication Date: 2017-01-11
宁波芸生纺织品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the existing key problems is that the existing silk fibroin dissolution process causes the silk fibroin to degrade greatly, which seriously affects the mechanical properties of the silk fibroin hydrogel and limits its application; the second problem is the preparation of the silk fibroin hydrogel. The extraction process is relatively complicated, including the following three main steps: 1. High-concentration neutral salt dissolution, 2. Long-term dialysis to obtain pure silk fibroin aqueous solution, 3. Inducing silk fibroin aqueous solution to coagulate to form a gel through internal and external factors; Third, the current silk fibroin hydrogel still has a sponge-like porous structure and does not have a biomimetic nanofiber structure, so its biological properties still need to be further improved, which cannot meet the needs of biological applications

Method used

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  • Nano-fibrosis silk fibroin gel and preparation method thereof
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  • Nano-fibrosis silk fibroin gel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) Natural mulberry silk was degummed by boiling 0.5wt% sodium bicarbonate solution for 30 minutes, and repeated 3 times to obtain degummed mulberry silk;

[0029] (2) Dissolve degummed silk in 2w / v calcium chloride-98wt% formic acid solution to obtain 10 w / v% silk protein solution;

[0030] (3) The above-mentioned silk fibroin solution was directly injected into a plastic tube, then immersed in deionized water, and nanofibrillated silk fibroin hydrogel was formed within 8 hours.

[0031] attached figure 1 It is a camera photo and a scanning electron microscope image after freeze-drying of the silk fibroin egg nanofiber hydrogel prepared above. It can be seen from the figure that the gel is milky white with a smooth surface, and the interior of the gel is mainly composed of silk fibroin nanofibrils. After mechanical compression test, the compressive modulus of the gel is 523.2KPa.

Embodiment 2

[0033] (1) Natural mulberry silk was degummed by boiling 0.05wt% sodium bicarbonate solution for 30 minutes, and repeated 3 times to obtain degummed mulberry silk;

[0034] (2) Dissolve degummed silk in 4w / v% calcium chloride-98wt% formic acid solution to obtain 25 w / v% silk protein solution;

[0035] (3) The above-mentioned silk fibroin solution was injected into a 24-well plate, and then immersed in deionized water, and nanofibrillated silk fibroin hydrogel was formed within 6 hours.

[0036] attached figure 2 , attached image 3 The X-ray diffraction spectrum and the scanning electron microscope picture after freeze-drying of the silk fibroin egg nanofiber hydrogel prepared above are respectively. It can be seen from the figure that the secondary structure of the silk protein is mainly a β-fold crystal structure, and the inside of the scaffold is composed of nano fiber composition. The compressive modulus of the gel was 16.2 MPa after mechanical compression test.

Embodiment 3

[0038] (1) Natural tussah silk was degummed by boiling with 0.5wt% sodium bicarbonate solution for 30 minutes, and the degummed tussah silk was obtained after repeating 3 times;

[0039] (2) Dissolving degummed tussah silk in 10w / v% lithium bromide-98wt% formic acid solution to obtain a silk protein solution with a concentration of 50w / v%;

[0040] (3) The above-mentioned silk fibroin solution was injected into a 6-well plate, then immersed in an aqueous solution, and tussah silk nanofibrillated silk fibroin hydrogel was formed within 20 hours.

[0041] attached Figure 4It is the scanning electron micrograph of the silk fibroin egg nanofiber hydrogel prepared above after freeze-drying. It can be seen from the figure that the interior of the hydrogel is also a nano-fibril structure. The compressive modulus of the gel was 98.2MPa after mechanical compression test.

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Abstract

The invention relates to a nano-fibrosis silk fibroin gel and a preparation method of the nano-fibrosis silk fibroin gel. Silk is degummed and then dissolved in salt-formic acid to obtain a silk fibroin solution; a silk fibroin-salt-formic acid solution is injected into a mould; the mould placed with the silk fibroin solution is immersed in an organic solvent or an aqueous solution, and stood for a certain time to form the silk fibroin gel. The preparation method disclosed by the invention is simple in preparation method, short in process, convenient to operate and easy for realization of batch production. The silk fibroin gel prepared by the invention has a natural bionic nanometer fibrillar structure, and is adjustable in mechanical properties and good in biocompatibility.

Description

technical field [0001] The invention relates to a nanofibrillated silk fibroin gel and a preparation method thereof. The prepared material can be applied to the fields of regenerative medicine such as tissue engineering and drug sustained release. Background technique [0002] There are millions of patients with organ or tissue damage and functional loss due to diseases and accidents every year. In the United States alone, more than 8 million operations are needed to treat such patients every year, and the economic cost is more than 400 billion US dollars. With the development of modern medicine and surgical techniques, tissue or organ transplantation to repair functional loss has been widely accepted, however, it faces a huge donor gap. The formation of tissues or organs in vivo or in vitro by means of regenerative medicine provides a new treatment option for the repair of damaged functions. Among them, the selection and construction of tissue engineering scaffold material...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/52A61L27/50A61L27/22C08J3/075
Inventor 张锋黄继伟左保齐
Owner 宁波芸生纺织品科技有限公司
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