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A kind of preparation method of effective part of Yemachai

An effective part, wild horse technology, applied in the field of traditional Chinese medicine and natural medicine, can solve the problems of composition defects, lack of large-scale equipment, high production cost of reversed-phase silica gel chromatography technology, etc., and achieve low production cost and green production process.

Active Publication Date: 2019-11-12
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main disadvantages of this patent are that, firstly, the preparation method of the sesquiterpene part disclosed in the application uses macroporous resin adsorption and ODS reversed-phase silica gel chromatography. In this preparation method, not only methanol and other toxic organic Solvent, and the production cost of reversed-phase silica gel chromatography technology is expensive (filler cost is about 6000-10000 yuan / kg), and large-scale equipment is lacking, so it is difficult to realize industrialization in the future; the second is that the effective part of Yemachai does not meet the requirements of "Chinese Pharmacopoeia" The sesquiterpene lactone component that must be contained - Yemazhu lactone A, there are also literatures that show that the sesquiterpene lactones in Yemazhu lactone are mainly Yemazhu lactone A and Yemazhu lactone B, so the composition of the effective part There are certain defects
[0007] In summary, although Yemazhui medicinal material and its preparations have been widely used clinically, there is no relevant research on the relatively simple and green preparation method of effective parts, which also limits the in-depth development and application of Yemazhui medicinal material

Method used

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  • A kind of preparation method of effective part of Yemachai
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  • A kind of preparation method of effective part of Yemachai

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Example 1 Preparation of the effective fraction of Yemazhui (1)

[0110] (1) extract

[0111] Take 5kg of Yemachai medicinal material, add 50% ethanol to extract twice, filter, recover the filtrate under reduced pressure, add water to make the medicinal solution contain 0.5g of crude drug per ml, and set aside; HPLC detection chart is attached figure 1 .

[0112] (2) Macroporous resin refining

[0113] Pass the above medicinal solution through AB-8 macroporous resin chromatographic column (5L), first elute with 2 times the column volume of water, then 2 times the column volume of 85% ethanol, collect the ethanol eluate, and recover ethanol under reduced pressure , concentrated to a relative density of 1.30 (50°C) thick paste 280g;

[0114] (3) Supercritical extraction and refining

[0115] Take 280g of thick paste, add 10 times the weight of adsorbent, mix well, dry, pulverize, put into supercritical extraction axe, with extraction pressure of 30MPa, extraction temp...

Embodiment 2

[0116] Example 2 Preparation of the effective fraction of Yemazhui (2)

[0117] (1) extract

[0118] Take 3kg of Ye Ma Zhui medicinal material, add water to decoct and extract with alcohol twice, filter, recover the filtrate under reduced pressure to 1g of crude drug per ml, and set aside;

[0119] (2) Macroporous resin refining

[0120] Pass the above medicinal solution through HPD-400 macroporous resin chromatographic column (4L), first elute with 2 times the column volume of water, then 2 times the column volume of 60% ethanol, collect the ethanol eluate, and recover ethanol under reduced pressure , concentrated to a relative density of 1.28 (50°C) thick paste 215g;

[0121] (3) Supercritical extraction and refining

[0122] Take 215g of thick paste, add 5 times the weight of adsorbent, mix well, dry, pulverize, put into supercritical extraction axe, with extraction pressure of 35MPa, extraction temperature of 50°C, entrainer (ethanol) dosage of 20%, separation pressure ...

Embodiment 3

[0123] Example 3 Preparation of the effective fraction of Yemazhui (3)

[0124] (1) extract

[0125] Take 5 kg of Ye Ma Zhui medicinal material, add 30% ethanol to extract twice, filter, recover the filtrate under reduced pressure to 0.5 g of crude drug per ml, and set aside;

[0126] (2) Macroporous resin refining

[0127] Pass the above medicinal solution through a D101 macroporous resin chromatographic column (8L), first elute with 2 times the column volume of water, then 3 times the column volume of 70% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure, concentrate To a relative density of 1.31 (50 ℃) thick paste 283g;

[0128] (3) Supercritical extraction and refining

[0129] Take 283g of thick paste, add 15 times the weight of adsorbent, mix well, dry, pulverize, put into supercritical extraction axe, with extraction pressure of 40MPa, extraction temperature of 40°C, entrainer (80% ethanol) dosage of 10%, The separation pressure is 7MPa, th...

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Abstract

The invention discloses a preparation method for a lindley eupatorium effective part and belongs to the technical field of traditional Chinese medicines and natural medicines. The method comprises the following steps: extracting lindley eupatorium with water or hydrous ethanol and adopting macro-porous resin and supercritical fluid extraction technique for acquiring lindley eupatorium terpene lactones part. The preparation method for the lindley eupatorium terpene lactones effective part is simple, no toxic reagent is used, the production process is green, safe and quick and the preparation method is suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine and natural medicine, and in particular relates to a preparation method of the effective part of Yemachai. Background technique [0002] Wild horse chasing is the dry aboveground part of Eupatorium lindleyanum DC. of Compositae, also known as white drum nails, herbivores, and Mao Zelan. It has the effects of clearing away heat and detoxification, eliminating phlegm and relieving asthma, lowering blood pressure, diuresis and reducing swelling. It is mainly used to treat chronic bronchitis, bronchitis, and hypertension. Yemazhui syrup made from it is widely used clinically. , chronic tracheitis, bronchitis has a very good effect. [0003] Modern studies have shown that the active parts of Yemazhui are mainly sesquiterpene lactones such as Yemai Lactone A and Yemai Lactone B. [0004] [0005] figure 1 Structural diagram of representative components of Yemazhui (1: Yemazhui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28
CPCA61K36/28A61K2236/333A61K2236/37A61K2236/51A61K2236/55
Inventor 郑云枫彭国平李存玉
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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