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Multicomponent surface enhanced Raman spectroscopy (SERS) detection method based on composite photonic crystal microspheres

A photonic crystal microsphere and surface-enhanced Raman technology, which is applied in the field of biomedical detection, can solve the problems of lack of Raman enhancement of photonic crystal encoded microspheres, inability to achieve multivariate analysis of detection range, and insufficient signal stability, and to achieve distinct, Stable signal and good uniformity

Active Publication Date: 2017-01-04
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the Raman-encoded microspheres and SiO 2 The photonic crystal encoded microsphere itself does not have the effect of Raman enhancement, but only plays the function of encoding
In summary, the existing SERS detection methods still have defects such as insufficient signal stability, low sensitivity, and poor reproducibility during detection, and cannot achieve multivariate analysis with a wide detection range.

Method used

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  • Multicomponent surface enhanced Raman spectroscopy (SERS) detection method based on composite photonic crystal microspheres
  • Multicomponent surface enhanced Raman spectroscopy (SERS) detection method based on composite photonic crystal microspheres
  • Multicomponent surface enhanced Raman spectroscopy (SERS) detection method based on composite photonic crystal microspheres

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Experimental program
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Effect test

Embodiment 1

[0027] (1) Preparation of composite photonic crystal microspheres: First, 200 μL (500 μg / mL) of streptavidin solution was added to 0.006 g of prepared silver nanoparticles-coated titanium dioxide photonic crystal microspheres at 4°C overnight, and 100 μL of 1% The bovine serum albumin was blocked at room temperature for 2 hours, and the blocked microspheres were washed with phosphate buffer solution before use; at the same time, 1 mL of the prepared three colors of green, yellow, and red water-phase CdTe quantum dots were respectively taken ( 0.8mmol / L) were mixed with biotin 200μL (1mg / mL) at room temperature, and placed in a shaker for 3h reaction; the above silver nanoparticles coated titanium dioxide photonic crystal microspheres modified with streptavidin into the three kinds of quantum dot solutions modified with biotin, shake the bed at room temperature for 3 hours, and finally wash three times with phosphate buffer solution;

[0028] (2) Immobilization of antibodies: e...

Embodiment 2

[0032] (1) Preparation of composite photonic crystal microspheres: First, 200 μL (500 μg / mL) of streptavidin solution was added to 0.007 g of prepared silver nanoparticles-coated titanium dioxide photonic crystal microspheres at 4°C overnight, and 100 μL of 1% The bovine serum albumin was blocked at room temperature for 2 hours, and the blocked microspheres were washed with phosphate buffer solution before use; at the same time, 1.4 mL of the prepared three colors of green, yellow, and red water-phase CdTe quantum dots were taken. (0.8mmol / L) were mixed with biotin 200μL (1mg / mL) at room temperature, and put into a shaker to react for 3h; the silver nanoparticles coated titanium dioxide photonic crystal microspheres modified with streptavidin were Add to the three kinds of quantum dot solutions modified with biotin, shake the bed at room temperature for 3 hours, and finally wash with phosphate buffer solution for 3 times;

[0033] (2) Immobilization of antibodies: 0.0075 g of ...

Embodiment 3

[0037] (1) Preparation of composite photonic crystal microspheres: First, 200 μL (500 μg / mL) of streptavidin solution was added to 0.008 g of prepared silver nanoparticles-coated titanium dioxide photonic crystal microspheres at 4°C overnight, and 100 μL of 1% The bovine serum albumin was blocked at room temperature for 2 hours, and the blocked microspheres were washed with phosphate buffer solution before use; at the same time, 1.6 mL of the prepared three colors of green, yellow, and red water-phase CdTe quantum dots were respectively taken. (0.8mmol / L) were mixed with biotin 200μL (1mg / mL) at room temperature, and put into a shaker to react for 3h; the silver nanoparticles coated titanium dioxide photonic crystal microspheres modified with streptavidin were Add to the three kinds of quantum dot solutions modified with biotin, shake the bed at room temperature for 3 hours, and finally wash with phosphate buffer solution for 3 times;

[0038] (2) Immobilization of antibodies:...

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Abstract

The invention discloses a multicomponent surface enhanced Raman spectroscopy (SERS) detection method based on composite photonic crystal microspheres. The multicomponent SERS detection method comprises the following steps of uniformly modifying quantum dots on the surfaces of titanium dioxide photonic crystal microspheres coated with sliver nanoparticles by an avidin-biotin system to generate an encodable composite photonic crystal microsphere material; combining the quantum dots with a titanium dioxide photonic crystal microsphere substrate material coated with the silver nanoparticles, and applying a combined substance in multicomponent surface enhanced Raman spectroscopy detection; realizing multielement detection for different biomolecules by combining encoding / decoding of the quantum dots with a Raman signal. According to the multicomponent SERS detection method disclosed by the invention, by using the titanium dioxide photonic crystal microspheres coated with the sliver nanoparticles as an encoding carrier, the multicomponent SERS detection method has the advantages of large specific surface area, high biocompatibility, simplicity and quickness in operation process and the like during biological detection; a fluorescence spectrum peak of the quantum dots is combined with a SERS characteristic spectral apex, so that the multicomponent SERS detection method can be effectively applied to multielement detection of biological detection.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a multi-component surface-enhanced Raman spectrum detection method based on composite photonic crystal microspheres. Background technique [0002] Early analysis and detection of tumor markers is of great significance in clinical diagnosis. Due to the complexity of carcinogenesis, many biomarkers are not specific to a cancer. Therefore, it is necessary to develop methods for simultaneous determination of multiple tumor markers. Compared with single-component detection, multiple detection technology simplifies the analysis process, shortens the analysis time, reduces the analysis cost, and makes it possible to obtain a huge amount of biomolecular information, which has become a research hotspot in recent years. [0003] Surface-enhanced Raman scattering (SERS) has been widely used in biological immunology and other fields as an analysis and detection meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李娟童晶晶李新卉李宛玲刁国旺
Owner YANGZHOU UNIV
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