A kind of detection method of mycoplasma gallisepticum

A technology of Mycoplasma gallisepticum and liquid culture medium, which is applied in the field of rapid cultivation and identification of Mycoplasma gallisepticum, can solve problems such as excessive generation of miscellaneous bacteria, hidden dangers of prevention and control, and reduced sample detection rate, and achieves good proliferation and growth, easy operation, Implement simple effects

Inactive Publication Date: 2019-12-24
LINYI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many problems in the detection of Mycoplasma gallisepticum, that is, the lack of a systematic and routine detection method, common detection methods focus on quality control, rather than speed, or do not focus on the actual effect of detection, and the yield rate of sample detection is low.
Since the isolation of pathogens requires multiple subcultures, value-added cultures, and identification with L-type bacteria, it takes at least one day to amplify one generation, and the time for detection and determination is too much. In addition, when used to produce vaccines, a large amount of thick meat is often used. Soup culture, the rapid value-added will also cause many samples to produce too many miscellaneous bacteria, so that thallium acetate can not be suppressed, so that the detection rate of many samples is greatly reduced, which brings great hidden dangers to the prevention and treatment of the disease

Method used

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  • A kind of detection method of mycoplasma gallisepticum
  • A kind of detection method of mycoplasma gallisepticum
  • A kind of detection method of mycoplasma gallisepticum

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A method for detecting mycoplasma gallisepticum, characterized in that:

[0031] (1) Preparation of Mycoplasma gallisepticum liquid medium:

[0032] Described Mycoplasma gallisepticum culture medium is prepared by following process:

[0033] Trypsinized casein 30g, glucose 15g, soybean papain digest 3g, yeast extract powder 15g, 1% thallium acetate solution 5ml. After mixing everything evenly, put it into a 500ml volumetric flask, dilute it to 500ml with deionized water, pour it out, and sterilize it at 120°C and 1.5 atmospheres for 30 minutes. After cooling to room temperature, add the following ingredients under sterile conditions: Filter-sterilized 5% arginine solution 8ml, sterile porcine serum 80ml, penicillin 800,000 units;

[0034] Use 0.1% sodium hydroxide solution to adjust the pH value to 7.0-7.2, and the above percentages are mass percentages;

[0035] (2) Collection of suspected disease materials:

[0036] Take at least 120 samples from sinus mucus, lary...

Embodiment 2

[0051] A method for detecting mycoplasma gallisepticum, characterized in that:

[0052] (1) Preparation of Mycoplasma gallisepticum liquid medium:

[0053] The Mycoplasma gallisepticum culture medium was prepared according to the following process: 20 g of trypsinized casein, 10 g of glucose, 5 g of soybean papain digest, 25 g of yeast extract powder, and 5 ml of 1% thallium acetate solution. After mixing everything evenly, put it into a 500ml volumetric flask, dilute it to 500ml with deionized water, pour it out, and sterilize it at 120°C and 1.6 atmospheres for 40 minutes. After cooling to room temperature, add the following ingredients under sterile conditions: Filter sterilized 5% arginine solution 10ml, sterile pig serum 10ml, penicillin 1 million units. Use 0.1% sodium hydroxide solution to adjust the pH value to 7.3-7.4, and the aforementioned percentages are all mass percentages;

[0054] (2) Collection of suspected disease materials:

[0055] Take at least 150 samp...

Embodiment 3

[0070] A method for detecting mycoplasma gallisepticum, characterized in that:

[0071] (1) Preparation of Mycoplasma gallisepticum liquid medium:

[0072] Described Mycoplasma gallisepticum culture medium is prepared by following process:

[0073] Trypsinized casein 15g, glucose 5g, soybean papain digest 6g, yeast extract powder 30g, 1% thallium acetate solution 5ml. After mixing everything evenly, put it into a 500ml volumetric flask, dilute it to 500ml with deionized water, pour it out, and sterilize it at 120°C and 2 atmospheres for 50 minutes. After cooling to room temperature, add the following ingredients under sterile conditions: Filter-sterilized 5% arginine solution 12ml, sterile pig serum 120ml, penicillin 1.2 million units. Use 0.1% sodium hydroxide solution to adjust the pH value to 7.1-7.3, and the above percentages are all mass percentages;

[0074] (2) Collection of suspected disease materials:

[0075] Take at least 160 samples from sinus mucus, larynx, an...

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Abstract

The technical problem to be solved in the present invention is to rapidly and efficiently proliferate Mycoplasma gallisepticum so as to be used for rapid detection of Mycoplasma gallisepticum pathogen. In order to achieve the purpose, the prevent invention is achieved by adopting the following technical scheme that the Mycoplasma gallisepticum detection method is characterized in that a Mycoplasma gallisepticum liquid culture medium is prepared, wherein yeast extract powder, glucose and trypticase are concurrently used in a soybean papain digest, such that the actual passage time shortening effect is more than 10% of the passage time shortening effect of the only use of the traditional yeast extract powder, the shortest passage time can be as fast as 12-16 h, and the passage time is 18 h in most cases. According to the present invention, the Mycoplasma gallisepticum liquid culture medium is adopted as the main proliferation base material of the formula so as to rapidly detect the Mycoplasma gallisepticum pathogen, such that the effective reference is provided for the definite diagnosis of the disease and the epidemic prevention work of the chicken flocks.

Description

technical field [0001] The invention belongs to the field of detection of pathogenic microorganisms, in particular to a rapid culture and identification method for mycoplasma gallisepticum. Background technique [0002] Mycoplasma Gallisepticum (MG) is also known as Mycoplasma gallisepticum. In broiler chickens, MG and other pathogenic bacteria can cause poultry chronic respiratory disease CRD, which can lead to increased mortality, elimination rate and waste rate during processing. ; In laying hens, in addition to the increase in mortality caused by the outbreak of MG, the real harm is that the egg production will drop by 5% to 10%. In turkeys, MG can cause sinusitis, air sacculitis and infectious sinusitis characterized by high mortality. The pathogen is widely distributed all over the world, bringing huge economic losses to the poultry industry; [0003] Mycoplasma is a group of microorganisms that can live independently between bacteria and viruses. Mycoplasma has hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12Q1/689C12R1/35
Inventor 孙晴张灵智黄霄李振
Owner LINYI UNIVERSITY
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