Method for extracting glutathione

A technology of glutathione and extraction method, applied in the direction of peptide, fermentation, etc., can solve the problems of high cost and high energy consumption, and achieve the effects of increasing production capacity, high product yield and high purity

Inactive Publication Date: 2017-01-04
河北美邦工程科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a glutathione extraction method to solve the problems of high cost and high energy consumption in the glutathione production process

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 A kind of glutathione extraction method, its steps are as follows:

[0020] (1) Enzyme catalysis and separation

[0021] According to the existing known synthesis method, L-glutamic acid, L-cysteine ​​and glycine are used as substrates, water and adenosine triphosphate are added, and Y--glutamic acid cysteine ​​synthetase GSHⅠ is added for catalysis, and then Add glutathione synthase GSH Ⅱ for catalysis, and obtain a mixed solution of glutathione and two enzymes through two-step reaction, and obtain glutathione aqueous solution and two enzymes with a mass concentration of 1.5% to 5% after separation , the enzyme returns to the system for reuse;

[0022] (2) Low-pressure reverse osmosis concentration

[0023] Send the glutathione aqueous solution with a mass concentration of 2% obtained in step (1) into the low-pressure reverse osmosis system, control the temperature of the system at 10-30°C, and the pressure at 1.5-2.0MPa, and reuse the water obtained on ...

Embodiment 2

[0030] Embodiment 2 A kind of glutathione extraction method, its steps are as follows:

[0031] (1) Enzyme catalysis and separation

[0032] According to the existing known synthesis method, L-glutamic acid, L-cysteine ​​and glycine are used as substrates, water and adenosine triphosphate are added, and Y--glutamic acid cysteine ​​synthetase GSHⅠ is added for catalysis, and then Add glutathione synthase GSH Ⅱ for catalysis, and obtain a mixed solution of glutathione and two enzymes through two-step reaction, and obtain glutathione aqueous solution and two enzymes with a mass concentration of 1.5% to 5% after separation , the enzyme returns to the system for reuse;

[0033] (2) Low-pressure reverse osmosis concentration

[0034] Send the glutathione aqueous solution with a mass concentration of 4% obtained in step (1) into a low-pressure reverse osmosis system, control the temperature of the system at 10-30°C, and the pressure at 1.5-2.0MPa, and reuse the water obtained on th...

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Abstract

The invention discloses a method for extracting glutathione. The method includes adding water, adenosine triphosphate, Y--glutamic acid and cysteine synthase GSH I into L-glutamic acid, L-cysteine and glycine which are used as substrates, carrying out catalysis, adding glutathione synthase GSH II into the substrates, carrying out catalysis, carrying out two-step reaction to obtain mixed solution with glutathione, the Y--glutamic acid and cysteine synthase and the glutathione synthase, separating the glutathione, the Y--glutamic acid and cysteine synthase and the glutathione synthase from one another to obtain low-concentration glutathione aqueous solution, the Y--glutamic acid and cysteine synthase and the glutathione synthase and allowing the Y--glutamic acid and cysteine synthase and the glutathione synthase to return systems to be reuse; carrying out low-pressure reverse osmosis concentration and high-pressure reverse osmosis concentration on the obtained low-concentration glutathione aqueous solution, then adding absolute ethyl alcohol into the glutathione aqueous solution, carrying out alcohol-precipitation crystallization on the glutathione aqueous solution to ultimately obtain glutathione products and recycling and reusing ethyl alcohol solution. The method has the advantages that high-concentration glutathione solution can be obtained by the aid of the method, the usage of the absolute ethyl alcohol can be reduced during alcohol-precipitation crystallization, energy consumption and the production cost can be decreased, the yield can be increased, the productivity can be improved, wastewater and waste gas can be prevented in production procedures, and accordingly the method is an environment-friendly, green and efficient production process.

Description

technical field [0001] The invention relates to a method for extracting a tripeptide compound, in particular to a method for extracting glutathione, and belongs to the technical field of organic compound production. Background technique [0002] Glutathione (glutathione, r-glutamylcysteingl+glycine, GSH) is a tripeptide containing γ-amide bonds and sulfhydryl groups, composed of glutamic acid, cysteine ​​and glycine. Molecular formula: C 10 h 17 N 3 o 6 S, molecular weight 307, crystals are colorless, transparent and elongated granular. Glutathione exists in almost every cell of the body and plays an important role in the biochemical defense system of the human body. The main physiological function is to remove free radicals in the human body. As an important antioxidant in the body, it protects many proteins and The sulfhydryl group in molecules such as enzymes is conducive to the development of enzyme activity, and can restore the active function of the -SH group in t...

Claims

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Application Information

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IPC IPC(8): C12P21/02C07K1/14C07K1/30
CPCC07K5/0215
Inventor 张玉新王骏刘东卢远峰闫利军王素霞
Owner 河北美邦工程科技股份有限公司
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