A differential temperature reaction chip and a temperature-controlled metal bath

Abstract

The invention discloses a differential-temperature reaction chip and a temperature-controlled metal bath. The differential-temperature reaction chip comprises a containing space arranged in a first substrate and a second substrate, the first substrate comprises a first main face and a second main face, the containing space is provided with a differential-temperature end capable of heating a solution flowing in at different heights at different temperatures to enable the solution to be in convection inside the containing space, and the second substrate is arranged on the first main face of the first substrate to seal the containing space. Through multipoint different-temperature heating, the differential-temperature reaction chip enables heated liquid to be in convection, so that heating speed is increased greatly; multiple solution buffering structures are arranged, so that flowing speed of the solution in the chip is ensured to be easy to control, and loss caused by overflowing of the solution is avoided.

Description

technical field [0001] The present invention relates to a sample analysis fluid chip, a sample analysis method, and a device for the sample analysis fluid chip used in detection or analysis of biological reactions. In particular, it relates to a disposable biochip and its device which can be used for protein or DNA analysis. Background technique [0002] The storage and reaction of biochemical reagents usually need to meet certain temperature conditions. The constant temperature metal bath is an instrument for temperature control for the storage and reaction of biochemical reagents. It is widely used in the storage of various biochemical samples, the storage of various enzymes and reaction, denaturation of nucleic acid and protein, PCR reaction (polymerase chain reaction), pre-denaturation of electrophoresis and serum coagulation, etc. [0003] The current constant temperature metal bath is composed of multiple devices that are closely attached from top to bottom, including...

AI Technical Summary

Problems solved by technology

The constant temperature metal bath of the prior art has defects in temperature adjustment, mainly manifested in poor thermal uniformity and slow heating and cooling speed

[0004] (1) Poor thermal uniformity

[0005] Since the gasket is generally made of aluminum, and the thermal conductivity of aluminum is only K=237W / m·K (W / m·degree), it cannot spread the heat quickly and uniformly, so its thermal conduction uniformity is not good enough; on the other hand , There are generally gaps in the contact surfaces between bases, gaskets, heating and cooling parts, and heat dissipation parts. The heat is transferred through the air with poor thermal conductivity, and the heat of the bonded part is transferred through the metal of the bonded surface, so the heat conduction effect of different parts will be quite different.

The uneven heat conduction caused by the above-mentioned various reasons makes the storage or reaction conditions of the reagents placed in different positions in the same base inconsistent, resulting in the failure of subsequent biochemical experiments to proceed smoothly.

[0006] (2) The heating and cooling speed is slow

When the reagent in the base of the constant temperature metal bath is heated, the reagent will heat up and generate steam. When the steam encounters the cold base cover, a large amount of steam and condensed water will be generated. When the condensed water formed by the steam flows back When it enters the base, it will cause contamination of the reagents in the base; on the other hand, when the base cover is opened, the condensed water on the base cover will flow down the edge of the base, causing waste of reagents. At the same time, the condensed water If it flows into the components under the base, it will corrode the components in the equipment and damage the circuit, so that the safety and service life of the constant temperature metal bath cannot be guaranteed

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