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A method for establishing a blue crystal flower bulblet regeneration system with petals as explants

A technology of small bulbs and explants, which is applied in the field of establishing a blue crystal flower bulb regeneration system, achieving the effects of low pollution rate, high callus induction rate, and improved induction rate and proliferation efficiency

Inactive Publication Date: 2018-06-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there is no report on the establishment of a blue crystal flower propagation system

Method used

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  • A method for establishing a blue crystal flower bulblet regeneration system with petals as explants
  • A method for establishing a blue crystal flower bulblet regeneration system with petals as explants
  • A method for establishing a blue crystal flower bulblet regeneration system with petals as explants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Extraction and processing of explants

[0038] During the flowering period of the blue crystal flower in June, young flower buds (buds not opened) with a length of 1.5-2.5 cm were picked, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 1 week.

[0039] Transfer the refrigerated flower bud material to a beaker, soak in water with a little Tween and detergent added dropwise for 15 minutes, then cover the mouth of the beaker with gauze, and rinse the gauze with running water for 1 hour. Soak the washed explants in (v / v) 75% ethanol for 30s, and then soak them in (w / v) 0.1% mercuric chloride for 8min. Reagents are fully exposed.

[0040] Finally, rinse 3 times with sterile water, each time not less than 3 minutes, until no obvious foam appears. Take out the explants with tweezers, place them on a sterile operating table, blot the surface moisture with filter paper, and wait for inoculation.

[0041] (2) Explant inoculation and callus induction

...

Embodiment 2

[0054] (1) Extraction and processing of explants

[0055] During the flowering period of the blue crystal flower in June, young flower buds (buds not opened) with a length of 1.5-2.5 cm were picked, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 1 week.

[0056] The refrigerated flower bud material was transferred to a beaker, soaked in water with a little Tween and detergent added dropwise for 15 minutes, then covered the mouth of the beaker with gauze, and rinsed with running water for 1.5 hours through the gauze. Soak the washed explants in (v / v) 75% ethanol for 30s, and then soak them in (w / v) 0.1% mercuric chloride for 10min. Reagents are fully exposed.

[0057] Finally, rinse 4 times with sterile water, each time not less than 3 minutes, until no obvious foam appears. Take out the explants with tweezers, place them on a sterile operating table, blot the surface moisture with filter paper, and wait for inoculation.

[0058] (2) Explant inoculation...

Embodiment 3

[0071] (1) Extraction and processing of explants

[0072] In the flowering period of the blue crystal flower in June, young flower buds with a length of 1.5-2.5 cm (buds are not opened) are collected, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 1 week.

[0073] The refrigerated flower bud material was transferred to a beaker, soaked in water with a little Tween and detergent added dropwise for 20 minutes, then covered the mouth of the beaker with gauze, and rinsed with running water for 1.5 hours through the gauze. Soak the washed explants in (v / v) 75% ethanol for 30s, and then soak them in (w / v) 0.1% mercuric chloride for 10min. Reagents are fully exposed.

[0074] Finally, rinse with sterile water 5 times, each time not less than 3 minutes, until no obvious foam appears. Take out the explants with tweezers, place them on a sterile operating table, blot the surface moisture with filter paper, and wait for inoculation.

[0075] (2) Explant inoculati...

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Abstract

The invention discloses a method for establishing a griffinia liboniana bulblet regeneration system by taking a petal as an explant. The method comprises the following steps: taking a griffinia liboniana flower bud, disinfecting, cutting the petal, inoculating the petal on a callus induction medium, culturing to obtain a callus tissue with a bulblet, peeling the bulblet from the callus tissue, transferring to a proliferation medium, culturing to obtain a bulblet proliferation body, and inoculating the bulblet proliferation body into a rooting medium to expand the bulblet and induce to root so as to obtain the griffinia liboniana bulblet. The petal is taken as the explant, the griffinia liboniana bulblet regeneration system is obtained through disinfecting, callus inducing, bulblet regenerating and proliferating, and bulblet expanding and rooting culture, so that the contamination rate is remarkably reduced, the induction rate and the proliferation efficiency are improved, and the expanding propagation period is shortened.

Description

technical field [0001] The invention relates to the field of rapid plant propagation, in particular to a method for establishing a blue crystal bulblet regeneration system with petals as explants. Background technique [0002] Griffinia (Griffinia) belongs to Amaryllidaceae (Amarylllidaceae), a tropical bulbous flower native to Brazil; it mostly grows in dense vegetation and humid tropical rainforest, with a diameter of 2-3cm, and is a miniature bulbous plant. Under natural growth conditions, aquamarine flowers generally form new plants by dividing the bulbs into lateral bulblets. Plants of this genus are not self-fertile, and reproduction by seed requires two different individuals, and it takes up to 8 months for the seeds to mature. Therefore, the long vegetative growth period and low natural reproduction coefficient have become two important factors restricting the proliferation of plants of the genus Azurite. [0003] In addition, due to the shrinking of the original f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 任梓铭夏宜平张栋李岳孙敏译吴昀
Owner ZHEJIANG UNIV
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