Sterile seed sowing method implemented after cracking of dendrobium officinale capsules

Abstract

The invention discloses a sterile seed sowing method implemented after cracking of dendrobium officinale capsules. Dendrobium officinale seed powder is collected into a conical bottle, a Tween solution is added, and standing is conducted for 4 h; seeds are filtered, collected, washed and then placed in a mercury bichloride solution to be soaked and disinfected for 15 min; the seeds are filtered, collected, washed and then sown on a culture medium. According to the method, the dendrobium officinale seeds are treated through soaking and disinfecting, the sterilization rate and the germination rate are both 90% or above, and the method is beneficial for improving the protection and utilization efficiency of wild dendrobium officinale resources and is used for stabilizing the property and quality of industrially-produced plants.

Description

technical field [0001] The invention relates to a method for aseptic sowing of seeds after dehiscence of Dendrobium officinale capsules, and belongs to the technical field of aseptic sowing of Dendrobium officinale seeds. Background technique [0002] Dendrobium officinate Kimura et Migo (Dendrobium officinate Kimura et Migo) is a perennial epiphytic herb of the orchid family Dendrobium, which grows on trees or forest edge rocks in forests at an altitude of 2100-2500m. Dendrobium candidum has the functions of nourishing yin and clearing heat, nourishing stomach and promoting body fluid, moistening lung and relieving cough, and has high medicinal value. In recent years, the wild resources of Dendrobium candidum have been excavated and destroyed in large quantities, and have been reduced year by year. They are now in an endangered state and are listed as key protected wild medicinal materials by the state. [0003] In the wild state, Dendrobium officinale grows slowly, and it...

AI Technical Summary

Problems solved by technology

However, since the capsules are inevitably damaged during cold storage, access, and operation, and the mature capsules of Dendrobium officinale are easy to crack, the above disinfection method is not suitable for the disinfection of small and large quantities of Dendrobium officinale seeds.