CpG ODN sequence with grass carp immune enhancement activity and use thereof

Abstract

The invention belongs to the field of genetic engineering of aquatic animals, and relates to a CpG ODN sequence with immune enhancing activity on grass carp and application thereof. Disclosed is a CpG ODN sequence that has immune enhancing activity on grass carp, the sequence is the nucleotide sequence shown in SEQ ID NO: 1, it contains the oligodeoxynucleotide sequence of the CpG motif, and the sequence contains three 'AACGTT' repeats, and a 'TCGA' palindromic sequence. Through preliminary screening of grass carp head kidney mononuclear cells and grass carp kidney cell lines, the enhancement effect of CpG on the immune activity of grass carp was verified, and the CpG with the strongest immune enhancement activity on grass carp cells was screened out through its activation effect on genes related to signaling pathways ODN sequence. Through the challenge test, it was verified again that the CpG ODN sequence has a strong immune enhancing effect. The sequence can be prepared into a grass carp immune enhancer, an immune adjuvant or a vaccine, and can be popularized and applied in the prevention of grass carp diseases.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering of aquatic animals, and in particular relates to a CpGODN sequence capable of enhancing immunity to grass carp and an application thereof. The invention is also relevant to the fields of aquatic animal immunology, molecular biology and cell biology. The invention also relates to the preparation of an immune enhancer with the CpG ODN sequence and its application in the prevention and treatment of related fish diseases. Background technique [0002] Grass carp (Ctenopharyngodon idella, English name grass carp) belongs to the genus Grass carp of Cyprinidae Cyprinidae. Grass carp is a large-scale economic fish of Cyprinidae unique to my country. It grows rapidly and has a wide range of feed sources. Therefore, it has become one of the four major freshwater fishes in China and has been successfully transplanted to many countries in Europe, Asia, Africa, and the United States. nation. In 2...

AI Technical Summary

Problems solved by technology

These new vaccines have high purity and strong specificity, but small molecules and weak immunogenicity make it difficult to induce an effective immune response in the body. Corresponding adjuvants are needed to enhance their immunogenicity or enhance the host’s protective response to antigens (Donnelly J J. New developments in adjuvants. Mechanisms of aging and development, 1997, 93(1): 171-177)

At present, the only adjuvant approved by the FDA that can be used in humans-aluminum colloid adjuvant can only stimulate humoral immunity, but cannot induce cell-mediated immune responses, and the latter is effective against parasitic pathogens (viruses, protozoa, etc.) It is especially necessary for tumors to develop immunity

Due to the limited immune adjuvant effect of aluminum colloidal adjuvant, it is far from meeting the requirements of new vaccine development

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