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Mycotoxin detecting rod

A mycotoxin and detection rod technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as unsuitable for on-site operation, time-consuming, complex and cumbersome processing, etc., to ensure detection accuracy and observation accuracy, improve detection limit, The effect of high detection sensitivity

Inactive Publication Date: 2016-12-21
成都测迪森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods for the detection of mycotoxins mainly include chromatography, mass spectrometry, LC-MS / MS, etc. Although these methods have high specificity and sensitivity, they generally have complex and tedious sample pretreatment, time-consuming, and are not suitable for on-site operation. Waiting for deficiencies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Such as Figure 1 to Figure 2 As shown, a mycotoxin detection stick includes a housing 1, the bottom of the housing 1 is provided with a sampling port 2, and the interior of the housing 1 is provided with a detection area 6, and the sampling port 2 is connected to the detection area 6 through the sensing area 5, and the sensing area 5 The inside is filled with a small amount of agglomerated particles, and a detection test paper is arranged in the detection area 6 . There are two channels in the sensing area 5, which are respectively channel one and channel two. There are agglomerated particles A in channel one, and agglomerated particles B in channel two. Both agglomerated particles A and agglomerated particles B are bonded to the channel wall through starch glue. superior. The sampling end 2 is provided with a suction pipe 4, the suction pipe 4 includes an air bag 3 located at the top, the air bag 3 is connected with the suction pipe, the air bag 3 is made of elastic ...

Embodiment 2

[0036] The preparation method of agglomerated particle A:

[0037] Immerse the washed bacterial cellulose in 4% copper sulfate solution for 12 hours and take it out, wherein the ratio of bacterial cellulose to copper sulfate is 8:1, and put it into the sodium borate solution protected by nitrogen under stirring conditions , Sodium borate solution Soak doxorubicin completely, according to the ratio of the amount of substances copper sulfate: sodium borate: ascorbic acid = 1:1:0.7 Slowly add sodium borate dropwise to the solution, react at 35°C for 30 minutes, add to the solution Ascorbic acid, after standing for 1~2h, the above product was rinsed several times with deionized water, and freeze-dried overnight to prepare the sample.

Embodiment 3

[0039] The preparation method of agglomerated particles B is as follows:

[0040] Immerse doxorubicin in 4% cobalt chloride solution for 12 hours and take it out, wherein the ratio of doxorubicin to cobalt chloride is 12:1, and put it into the sodium hydroxide solution protected by nitrogen under stirring conditions , Sodium hydroxide solution to completely soak doxorubicin, according to the ratio of cobalt chloride: sodium hydroxide: ascorbic acid = 1:1:0.7, slowly add sodium hydroxide to the solution, and react at 35°C for 30 minutes, Ascorbic acid was added to the solution, and after standing for 1-2 hours, the above product was rinsed several times with deionized water, and freeze-dried overnight to prepare a sample.

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Abstract

The invention discloses a mycotoxin detecting rod, and belongs to the field of test paper. The mycotoxin detecting rod disclosed by the invention comprises a housing, wherein a sample inlet end is arranged at the bottom of the housing; the interior of the housing is provided with a detection area; the sample inlet end is connected with the detection area through an induction area; the induction area is filled with a small quantity of inductive agglomerated particles; and the detection area is internally provided with test paper. The mycotoxin detecting rod disclosed by the invention is convenient to use, quick and sensitive, complex pretreatment is not needed for a sample, whether ochratoxin is contained in the sample can be quickly detected, the detection sensitivity is relatively high, and the detection limit can be up to 1.4 ng / mL.

Description

technical field [0001] The invention relates to a detection test paper, in particular to a mycotoxin detection stick. Background technique [0002] Mycotoxins are secondary toxic metabolites produced by fungi, which are commonly found in feed and food. They mainly cause harm to humans or animals through the food supply link, including causing common inflammatory reactions such as gastroenteritis, and changing the surface of macrophages. Type and DNA damage, even highly carcinogenic, and will cause damage to the reproductive system of humans or animals, and have a serious impact on children's growth and development. Mycotoxins have a wide range of contamination, which can be crops such as wheat and corn, fresh aquatic products, or milk and coffee used by people in daily life. Existing methods for the detection of mycotoxins mainly include chromatography, mass spectrometry, LC-MS / MS, etc. Although these methods have high specificity and sensitivity, they generally have comple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/544
CPCG01N33/544G01N2333/38
Inventor 郑浩
Owner 成都测迪森生物科技有限公司
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