Salmonella rapid detection culture medium and detection vessel

Abstract

The invention discloses a salmonella rapid detection culture medium and a detection vessel. The culture medium is prepared from water absorbent gel, tryptone, beef extract powder, yeast extract powder, sodium chloride, cholate, a specific chromogenic substrate, IPTG (Isopropyl-beta-d-thiogalactoside), novobiocin and a stabilizer. The salmonella rapid detection culture medium provided by the invention can quickly absorb a sample solution, and imbibes to form jelly sample culture medium gel within 2 minutes. The salmonella rapid detection culture medium provided by the invention can also well absorb an alcoholic sample diluent, so that the sample diluent can be uniformly distributed on the surface of the culture medium, liquid leakage caused by misoperation is reduced, and the operation during use is greatly simplified. The culture medium absorbs the sample diluent to form solid gel, and colored undissolved substances generated through the reaction of the specific chromogenic substrate can remain around microorganisms, so that diffusion and trailing can be avoided, and a result can be observed easily; a positive salmonella bacterial colony is colored in purple red, and other unsubdued coli groups are colored in blue-green.

Description

technical field [0001] The invention relates to a rapid detection culture medium and a detection dish for microorganisms. Background technique [0002] Microorganisms are ubiquitous in nature and are closely related to people's production and life. Food contamination by microorganisms will cause food spoilage, causing great losses to manufacturers and health threats to consumers. Therefore, food must undergo a series of microbiological tests to ensure people's food safety. The traditional microbial detection method is generally the plate detection method, etc., and the agar medium is added to the plate. This method is complicated and cumbersome to operate and is easy to contaminate. The water in the medium is easy to lose, and the lost water condenses and accumulates on the wall of the petri dish, which is easy to cause bacterial colony contamination, and the petri dish method cannot meet the growing demand for detection. [0003] Salmonella is a zoonotic pathogenic microo...

AI Technical Summary

Problems solved by technology

[0006] There are certain deficiencies in the existing Salmonella chromogenic medium. For example, it needs to be prepared immediately when using it, which is time-consuming and laborious, which affects the progress of the experiment, and it is difficult to meet the requirements of fast, accurate and reliable.

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