Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of serum-free medium and basal medium, which is applied in the direction of cell culture active agent, culture process, tissue culture, etc. It can solve the problems of unstable medium components, achieve good functional consistency, stable components, and increase the value-added multiple Effect
Inactive Publication Date: 2016-12-21
英普乐孚生物技术(上海)有限公司
View PDF3 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0008] In view of the above-mentioned problems and / or other problems in the related art, the present invention provides a serum-free medium for DC cells with more stable components, which improves the instability of the medium components caused by the complex composition of albumin
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1～4
[0027] The preparation process of DC cell serum-free medium in Examples 1-4 is as follows:
[0028] Step 1: Obtain fatty acid-free albumin;
Embodiment 1
[0029] Embodiment 1 Get bovine serum albumin (the A1933 product purchased from SIGMA company) 1.5mmol, add water for injection and be made into 2000ml solution, add 50g gac, mix; Adopt the hydrochloric acid solution of 2mol / L to adjust the pH value to pH3.0; Place it in a water bath at 56°C for 30 minutes or place it overnight at 4°C; centrifuge (4000 rpm) to remove the activated carbon; then use 2mol / L NaOH solution to adjust the pH value to pH7.0; finally filter and sterilize to obtain the product of Example 1 fatty acid-free albumin solution;
Embodiment 2
[0030] Example 2 Take human serum albumin (purchased from Grifols (Grifols) company's human serum albumin (the product specification is 20% 50ml*10g / bottle, wherein the content of sodium octanoate is not more than 5mol / mol albumin)) 1.5 mmol, the specific operation process is the same as in Example 1, and the fatty acid-free albumin solution of Example 2 is obtained;
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
concentration
aaaaa
aaaaa
Login to view more
Abstract
The invention relates to a serum-free medium for dendritic cells (DCs), and a preparation method of the serum-free medium. The preparation method comprises the steps of loading fatty acid onto fatty acid-free albumin, and then adding the obtained albumin solution into a basic medium. The obtained serum-free medium for the DCs is more stable in components and better in functional consistency, and difference cannot be caused by different species origins or processing batches of the albumin.
Description
technical field [0001] The invention relates to a DC cell serum-free medium, which belongs to the technical field of cell culture. Background technique [0002] Serum-free medium refers to a synthetic medium that can maintain the growth and reproduction of cells in vitro for a long time without adding serum, and can be used to cultivate immune cells, such as lymphocytes (for example, T lymphocytes, B cells, NK cells, etc.) , dendritic cells (also known as DC cells), monocytes / macrophages, etc. [0003] Generally speaking, the nutrients required for the survival and expansion of immune cells in vitro include: albumin, transferrin, insulin, amino acids, vitamins, sugars, lipids, inorganic salts, growth factors, etc. Among these components, albumin has the greatest impact on cell growth and survival, and is also the component with the most complex function and composition. In serum-free medium, the function of albumin is mainly determined by the fatty acids and trace elements...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more