A microfluidic chip and its application in pathogen identification and drug sensitivity test

Abstract

The invention discloses a micro-fluidic chip and application thereof in authentication of pathogene and susceptibility testing. The characteristic that an agar culture-medium has high-temperature digestion and low-temperature solidification is utilized, an authenticating culture medium is placed on the middle layer of the chip, an upper layer chip concentration gradient generator is utilized, and a drug to be studied is introduced; the drug is separated in different culture ponds, multiple pathogene analysis is achieved through the space resolving power of a culture pond array, pathogene authentication is achieved according to the specificity developing result, pathogene quantifying is achieved through real-time developing strength analysis, and the drug susceptibility is determined according to the lowest antibiotic concentration of a developing inhibiting reaction. The micro-fluidic chip is especially suitable for pathogene analysis under the deficient medical resource condition, and has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of pathogen identification and drug sensitivity test, and in particular relates to a microfluidic chip and a method for synchronously realizing cervical pathogen detection and drug sensitivity test using the microfluidic chip. Background technique [0002] In recent years, breakthroughs have been made in the biological factors of cervical cancer. A large number of epidemiological and molecular biology studies have concluded that some pathogens, especially those transmitted through sexual transmission, are closely related to the occurrence of cervical cancer. In gynecological diseases, A disease with the highest incidence rate is chronic cervicitis and vaginitis. At present, for the treatment of these diseases, most hospitals still use western medicine as the main treatment, but with the continuous development of society, traditional Chinese medicine is gradually applied to the treatment of chronic cervicitis...

AI Technical Summary

Problems solved by technology

Methods for determining minimum inhibitory concentration guidelines are published by the Institute for Clinical and Laboratory Standards. These standardized tests are reliable but cumbersome to perform and require long incubation times (typically 16 to 20 hours) until the MIC can pass Visual detection of culture turbidity or colony growth

This is not conducive to timely diagnosis and antibiotic selection guidance

[0004] The traditional bacterial identification method is to smear the patient's body fluid specimen on the agar plate containing the culture medium to culture and enrich the bacteria, and then select the dominant bacteria for culture identification and conduct drug sensitivity test. The problem of this method is that the sample consumption is large and the detection time is long. , often cannot effectively meet the needs of clinical work. In addition, most of the traditional bacterial identification methods rely on numerous large-scale specialized equipment, which limits the promotion of this technology in primary medical units.

Chromogenic culture medium overcomes the disadvantages of traditional culture medium, such as complicated (experienced technicians are required), low sensitivity and poor specificity in various bacterial isolation, identification, counting and other operations

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