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A method for removing stickies in waste paper pulp by using high-temperature esterase

A waste paper pulp and esterase technology, applied in biochemical equipment and methods, waste paper processing, recycling technology, etc., can solve problems such as low efficiency, high energy consumption, and easy inactivation of common biological enzymes in industrial wastewater, and achieve technological Simple, low cost, and the effect of solving the problem of product quality stickies

Active Publication Date: 2019-07-12
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of high energy consumption, low efficiency, easy production of a large amount of industrial waste water and easy inactivation of common biological enzymes in high temperature environment in the existing waste paper stickies removal process

Method used

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  • A method for removing stickies in waste paper pulp by using high-temperature esterase
  • A method for removing stickies in waste paper pulp by using high-temperature esterase
  • A method for removing stickies in waste paper pulp by using high-temperature esterase

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preparation example Construction

[0029] The preparation process of esterase EST1: the encoding gene of EST1 esterase obtained by the whole gene synthesis method is cloned into the expression vector pET23a-CBD vector to obtain the recombinant expression vector. Using CaCl 2 In the transformation method, the expression plasmid containing the EST1 esterase coding gene is transferred into the BL21 (DE3) strain to obtain the genetically engineered strain. Inoculate the strain of esterase EST1 into the LB seed medium containing Amp (100ug / ml) to expand the culture, and when the OD value reaches 0.6-0.8, inoculate the bacterial solution into the LB seed medium containing Amp (100ug / ml) at a ratio of 1:100. ml) of LB fermentation medium, shake until the OD value is 0.6-0.8, add IPTG (20mmol / L) inducer to induce expression, and harvest bacteria after 18-24h. Centrifuge the bacterial solution at 12000r / min for 10min, collect the cells on the tube wall for ultrasonic disruption, then centrifuge at 12000r / min for 10min,...

Embodiment 1

[0032] Weigh 100g OCC dry pulp, dilute the pulp to 4%, the amount of esterase EST1 added is 0.2U / g (relative to the dry pulp), the treatment temperature is 40°C, the pH is 5.0, the treatment time is 45min, and the stirring speed is 100rpm, the embodiment uses the Pulmac-MasterScreen sieving instrument to screen the slurry according to the TAPPIT-277 sticky matter assay method, then dyes and compresses the sieved sticky matter, and finally analyzes and scans it with scanning software, and then measures The content of stickies after enzyme treatment was obtained. In the case of not adding enzymes, the waste paper pulp was treated with the same conditions, and the experimental results were the stickies content of the blank group.

Embodiment 2

[0034] Weigh 100g of OCC dry pulp, dilute the pulp to 5%, the amount of esterase EST1 added is 0.5U / g (relative to the dry pulp), the treatment temperature is 55°C, the pH is 5.0, the treatment time is 60min, and the stirring speed is 120rpm, the embodiment uses the Pulmac-MasterScreen sieving instrument to screen the slurry according to the TAPPIT-277 sticky matter assay method, then dyes and compresses the sieved sticky matter, and finally analyzes and scans it with scanning software, and then measures The content of stickies after enzyme treatment was obtained. In the case of not adding enzymes, the waste paper pulp was treated with the same conditions, and the experimental results were the stickies content of the blank group.

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Abstract

The invention discloses a method for removing sticky substances in waste paper pulp by utilizing high-temperature esterase. The esterase is esterase EstE1, PDB ID: 2C7B from a metagenome library. The method specifically comprises the following steps: adding 0.1U / g to 1.0U / g (relative to absolute dry pulp mass) of the esterase into the waste paper pulp, wherein the treatment time is 45min to 120min, the temperature is 40 DEG C to 80 DEG C, the pH (Potential of Hydrogen) is 5.0 to 9.0, and the stirring speed is 100rpm to 150rpm; finally, detecting the content of the sticky substances in the waste paper pulp according to a TAPPIT-277 standard method. A result proves that the removal rate of the sticky substances reaches 54.1 percent to 66.5 percent; the removing effect in a high-temperature environment is still relatively good. The sticky substances in the waste paper pulp are effectively degraded through utilizing the high-temperature esterase EST1; an operation process is simple and convenient, and extra mechanical equipment is not needed; the removing effect on the sticky substances is obvious, the production cost is low, and no pollution is caused.

Description

technical field [0001] The invention relates to a method for removing stickies in waste paper pulp by using high-temperature esterase. Background technique [0002] In recent years, waste paper pulp has accounted for more than 60% of the total global pulp consumption. Waste paper is an important raw material in my country's pulp and paper industry. The reuse of waste paper can not only save a large amount of plant fiber raw materials and reduce production costs, but also has far-reaching significance for realizing energy saving and reducing consumption and mitigating environmental pollution. However, the problem of the formation and control of stickies has become increasingly prominent in the actual waste paper recycling process. Stickies refer to the various sources in the process of waste paper recycling, which have permanent or temporary stickiness and can be caused in the papermaking process. Problems and all substances that cause degradation of product quality. Sticki...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D21C5/00D21C5/02C12N9/18
CPCC12N9/18C12Y301/01D21C5/005D21C5/02Y02W30/64
Inventor 王永华蓝东明张泽栋杨博周鹏飞
Owner SOUTH CHINA UNIV OF TECH
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