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Culture medium for increasing proliferation rate of sub-omnipotent stem cells

A sub-totipotent stem cell and culture medium technology, which is applied in the field of culture medium to increase the proliferation speed of sub-totipotent stem cells, can solve the problems of pollution, residue, poor cell adhesion, etc., and achieve the effect of increasing growth rate, less dead cells, and increasing the number

Inactive Publication Date: 2016-12-14
ANHUI HUIEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The sub-totipotent stem cell culture medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, non-human-derived substances are mixed into the stem cell culture medium, causing residue or pollution, and the existing sub-totipotent stem cell culture There are problems such as poor cell adhesion and unsatisfactory cell proliferation speed.

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0015] A culture medium for improving the proliferation rate of subtotipotent stem cells proposed by the present invention includes DMEM medium; it also includes: hydroxyethyl starch 3g / L, lipoic acid 38mg / L, potassium bicarbonate 0.5g / L, diethylamine tetra Sodium acetate 0.6 μg / L, hyaluronic acid 2 μg / L, taurine 8 μg / L, insulin-like growth factor 0.9 g / L, recombinant human insulin 0.6 g / L, placental growth factor 15 μg / L, platelet-derived growth factor 30mg / L, glutathione 60mg / L, vitamin 80mg / L, plant extract 80mg / L.

Embodiment 2

[0017] A culture medium for improving the proliferation rate of sub-totipotent stem cells proposed by the present invention, including DMEM medium; also includes: hydroxyethyl starch 3.5g / L, lipoic acid 30mg / L, potassium bicarbonate 0.6g / L, diethylamine Sodium tetraacetate 0.3 μg / L, hyaluronic acid 5 μg / L, taurine 5 μg / L, insulin-like growth factor 1.2 g / L, recombinant human insulin 0.3 g / L, placental growth factor 30 μg / L, platelet-derived growth Factor 20mg / L, glutathione 90mg / L, vitamin 40mg / L, plant extract 120mg / L.

[0018] The vitamins include by weight: 12 parts of vitamin C, 17 parts of vitamin B family, 3 parts of coenzyme Q10, and 8 parts of vitamin E. Vitamin B family is a combination of thiamine and riboflavin.

Embodiment 3

[0020] A culture medium for improving the proliferation rate of subtotipotent stem cells proposed by the present invention includes DMEM medium; also includes: hydroxyethyl starch 3.2g / L, lipoic acid 36mg / L, potassium bicarbonate 0.52g / L, diethylamine Sodium tetraacetate 0.5 μg / L, hyaluronic acid 3 μg / L, taurine 7 μg / L, insulin-like growth factor 1 g / L, recombinant human insulin 0.5 g / L, placental growth factor 20 μg / L, platelet-derived growth factor 27mg / L, glutathione 70mg / L, vitamin 70mg / L, plant extract 90mg / L.

[0021] The vitamins include by weight: 14 parts of vitamin C, 15 parts of biotin, 5 parts of coenzyme Q10, and 6 parts of vitamin E.

[0022] The plant extracts include by weight: 2 parts of dandelion sterols, 6 parts of tea polyphenols, 12 parts of polysaccharides of poria cocos, 20 parts of astragalus polysaccharides and 13 parts of polysaccharides of Codonopsis pilosula.

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Abstract

The invention also discloses a culture medium for increasing the proliferation rate of sub-omnipotent stem cells. The culture medium comprises a DMEM culture medium, and further comprises the following components: 3-3.5 grams per liter of hydroxyethyl starch, 30-38 milligrams per liter of lipoic acid, 0.5-0.6 grams per liter of potassium bicarbonate, 0.3-0.6 micrograms per liter of sodium ethylenediamine tetraacetate, 2-5 micrograms per liter of hyaluronic acid, 5-8 micrograms per liter of taurine, 0.9-1.2 grams per liter of insulin-like growth factor, 0.3-0.6 grams per liter of recombinant human insulin, 15-30 micrograms per liter of placental growth factor, 20-30 milligrams per liter of platelet-derived growth factor, 60-90 milligrams per liter of glutathione, 40-80 milligrams per liter of vitamin and 80-120 milligrams per liter of plant extract.

Description

technical field [0001] The invention relates to the technical field of stem cell culture medium, in particular to a culture medium for improving the proliferation speed of subtotipotent stem cells. Background technique [0002] The sub-totipotent stem cell culture medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, non-human-derived substances are mixed into the stem cell culture medium, causing residue or pollution, and the existing sub-totipotent stem cell culture There are problems such as poor cell adhesion and unsatisfactory cell proliferation speed. Contents of the invention [0003] Based on the technical problems existing in the background technology, the present invention proposes a culture medium for increasing the proliferation speed of sub-totipotent stem cells, which has good cell adhesion and fast proliferation speed, improves the anti-pathogenic microorganism effect of the culture medium, and has no ani...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0602C12N2500/30C12N2500/38C12N2501/10C12N2501/105C12N2501/135C12N2501/33C12N2501/90C12N2501/905C12N2501/998
Inventor 张正亮
Owner ANHUI HUIEN BIOTECH
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