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A quality control method capable of simultaneous content analysis and similarity evaluation of 18 components in Kudingcha

A Kudingcha and detection method technology, which is applied in the direction of analyzing materials, measuring devices, material separation, etc., can solve problems such as difficult popularization of determination methods and bottlenecks of reference substances, etc.

Active Publication Date: 2019-01-11
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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Problems solved by technology

[0004] However, most of the quantitative methods currently reported are limited by the bottleneck problem of reference substances, especially most of the triterpenoid saponins are self-made in the laboratory, and traditional determination methods are difficult to popularize
Therefore, the quality inspection and supervision of Kudingcha remains a challenge

Method used

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  • A quality control method capable of simultaneous content analysis and similarity evaluation of 18 components in Kudingcha
  • A quality control method capable of simultaneous content analysis and similarity evaluation of 18 components in Kudingcha
  • A quality control method capable of simultaneous content analysis and similarity evaluation of 18 components in Kudingcha

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Embodiment 1

[0116] Example 1 The quality control method that can simultaneously realize the content analysis and similarity evaluation of 18 components in Kudingcha

[0117] 1. Instruments and reagents

[0118] 1.1 Instruments:

[0119] Waters Alliance HPLC, Agilent 1260 HPLC, Shimadzu LC-20A HPLC, Phenomenex Synergi Hydro-RP C18 column, Waters Symmetry C18 column and ACCHROM C18 column.

[0120] 1.2 Reagents and medicines:

[0121] Rutin on 6-hydroxy-7,7a-dihydro-2(6H)-benzofuran, hydroxytyrosol glucoside, picridoside E, picridoside D, picridoside A, big-leaf holly Glycoside G, Kudingcha glucoside G, Kudingcha glucoside T and Dayedongqing glycoside H are isolated from Kudingcha. Protocatechuic acid, isochlorogenic acid A, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid B, and isochlorogenic acid C were purchased from China Institute of Pharmaceutical and Biological Products or Sichuan Weiwei Qi Biological Technology Co., Ltd.; 15 batch...

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Abstract

The invention discloses a quality control method for simultaneous realization of content analysis and similarity evaluation of 18 components in Ilex kudingcha. Rutin, isochlorogenic acid A and kudinoside A are used as internal references; correction factors of rutin for 6-hydroxy-7,7a-dihydro-2(6H)-benzofuran and hydroxytyrosol glucoside, correction factors of isochlorogenic acid A for protocatechuic acid, kudinoside E, kudinoside D, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid B, and isochlorogenic acid C, and correction factors of kudinoside A for latifoloside G, kudinoside G, ilex kudingcha ilexoside T and latifoloside H are calculated, and the factors are used as constants for determining content. Only three common reference substances are needed for simultaneous determination of contents of 18 kinds of components in ilex kudingcha, quality of the ilex kudingcha can be rapidly, economically and scientifically controlled, and further cluster analysis, main component analysis and similarity calculation of medicinal materials can be carried out by using contents of the 18 kinds of components, in order to comprehensively control quality of ilex kudingcha.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine analysis. More specifically, it relates to a quality control method that can simultaneously realize the content analysis and similarity evaluation of 18 components in Kudingcha. Background technique [0002] Kudingcha is the dried leaves of the genus Ilex in the family Ilex, widely distributed in the provinces of Hainan, Hubei, Guangdong, Guangxi, and Hunan in China. As a national medicine for the prevention of diseases related to abnormal lipid metabolism, it has been used in the folk for thousands of years. Current research shows that it has significant pharmacological activity against cardiovascular diseases and metabolic syndrome, and has become a hot spot in the current research on the homology of medicine and food. Based on its anti-oxidative and immunomodulatory properties, Kudingcha is usually used clinically as an adjuvant treatment for diabetes, hypertension, obesit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 张蕾周杰邝国俊赵钟祥
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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