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Dual-signal electrochemical sensor for detecting doxorubicin hydrochloride rate based on bare glassy carbon electrode

A glassy carbon electrode, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of high cost, complicated operation, etc., and achieve the effects of fast response, simple operation, and simple preparation method

Active Publication Date: 2016-12-07
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, commonly used methods for detecting DOX include fluorescence spectroscopy, ultra-high performance liquid chromatography, liquid-liquid extraction-high performance liquid chromatography, and chip capillary electrophoresis fluorescence detection, among which the invention patent "Determination of DOX in body fluid based on magnetic nano-adsorbent Method" (Patent No. 201310445367.X) adopts the chemical co-precipitation method and prepares the magnetic nano-adsorbent TSAB-coated Fe through layer-by-layer assembly 3 o 4 / SiO 2 , to detect the content of DOX in plasma and urine samples, the magnetic adsorbent prepared by this method has good stability, large adsorption capacity, and can be reused; but the operation is complicated and the cost is high

Method used

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  • Dual-signal electrochemical sensor for detecting doxorubicin hydrochloride rate based on bare glassy carbon electrode
  • Dual-signal electrochemical sensor for detecting doxorubicin hydrochloride rate based on bare glassy carbon electrode
  • Dual-signal electrochemical sensor for detecting doxorubicin hydrochloride rate based on bare glassy carbon electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039](1) Prepare a PBS buffer solution with a concentration of 0.01M and a pH of 6.5. Take 50 μL of 1 mM MB solution dropwise into the PBS buffer solution under dark conditions. Under the same dark conditions, take different amounts of 200 μM DOX dropwise into the PBS solution of the above MB, and ultrasonically mix and disperse to prepare concentration gradients of 0.01 μM, 0.05 μM, 0.1 μM, 0.5 μM, 1 μM, 2 μM, and 3 μM. and 4 μM.

[0040] (2) The glassy carbon electrode is successively coated with 0.5 μm and 0.03 μm Al 2 o 3 The powder is ground into a mirror surface, then cleaned with deionized water, and then placed in deionized water and ethanol for 1 to 2 minutes of ultrasonication; the treated glassy carbon electrode is immersed in potassium ferricyanide solution for cyclic voltammetry scanning, so that the oxidation The reduction peak potential difference is less than 80mV; after the scanning is completed, perform the above-mentioned grinding and polishing steps aga...

Embodiment 2

[0041] Embodiment 2: the mensuration of actual sample

[0042] (1) Dilute human serum ten times with 0.01M PBS buffer solution, pH 6.5. Take 50μL, 1mMMB solution drop by drop into the human serum solution under dark conditions. Under the same dark condition, a certain amount of DOX was dropped into the above-mentioned MB human serum solution, and ultrasonically mixed.

[0043] (2) The steps of measuring the SWV signals of DOX and MB in the human serum solution with a glassy carbon electrode are the same as the step (2) of Example 1, and the content of DOX is determined according to the drawn standard curve.

[0044] The above-mentioned embodiment is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above-mentioned embodiment, and any other changes, modifications, substitutions, combinations, Simplifications should be equivalent replacement methods, and all are included in the protection scope of the present inv...

Embodiment 3

[0046] (1) Prepare a PBS buffer solution with a concentration of 0.03M and a pH of 7.0. Take 50 μL of 0.1 mM MB solution drop by drop into the PBS buffer solution under dark conditions. Under the same dark conditions, take different amounts of 100 μM DOX dropwise into the PBS solution of the above MB, and ultrasonically mix and disperse to prepare concentration gradients of 0.01 μM, 0.05 μM, 0.1 μM, 0.5 μM, 1 μM, 2 μM, and 3 μM. and 4 μM.

[0047] (2) The glassy carbon electrode is successively coated with 0.5 μm and 0.03 μm Al 2 o 3 The powder is ground into a mirror surface, then cleaned with deionized water, and then placed in deionized water and ethanol for 2 minutes for ultrasonication; the treated glassy carbon electrode is immersed in potassium ferricyanide solution for cyclic voltammetry scanning, so that the redox peak The potential difference is less than 80mV; after the scanning is completed, perform the above-mentioned grinding and polishing steps again, and blo...

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Abstract

The invention discloses a dual-signal electrochemical sensor for detecting a doxorubicin hydrochloride rate based on a bare glassy carbon electrode. A specific detection method comprises the steps of immersing the glassy carbon electrode into phosphatic water buffer solution containing DOX and methylene blue (MB), correlating a ratio delta IDOX / delta IMB between the electrochemical signal intensities of two substrates with the concentration of the DOX, and drawing a linear standard curve; measuring an SWV signal of a sample containing the DOX and the MB and determining the content of the DOX in serum according to the drawn standard curve. The prepared dual-signal electrochemical sensor for detecting the DOX rate based on the bare glassy carbon electrode is simple to operate, high in flexibility and good in stability and has important significance in detecting the dosage of the DOX in the serum of a cancer patient.

Description

technical field [0001] The invention relates to an electrochemical sensor for detecting antitumor drugs, in particular to a ratio dual-signal electrochemical sensor for detecting antitumor drug doxorubicin hydrochloride based on bare glassy carbon electrodes, and belongs to the technical field of biological detection. Background technique [0002] Due to the increasing aging of the population, the destruction of the ecological environment, and unhealthy lifestyles and other factors, the number of cancer patients is increasing year by year. In recent years, tumor chemotherapy has made considerable breakthroughs, and the survival time of tumor patients has been greatly extended, especially in the treatment of malignant tumors such as leukemia and malignant lymphoma. The treatment of solid tumors, which account for more than 90% of malignant tumors, has not yet achieved satisfactory results. Doxorubicin hydrochloride (DOX) is one of the most widely used anthracycline antibioti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/308G01N27/48
Inventor 桂日军金辉于建波徐显朕王宗花
Owner QINGDAO UNIV
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