Free fatty acid determining kit and preparation method thereof

Abstract

The invention relates to the technical field of free fatty acid determining, in particular to a free fatty acid determining kit. The kit comprises a reagent R1 and a reagent R2, wherein the R1 is prepared from coenzyme A, triphosadenine, acyl coenzyme A synthetase, MgCl2, a Trinder substrate and Tween-20, and has the pH of 7.2; the R2 is prepared from flavin adenine dinucleotide, 4 amino-antipyrine, peroxidase, acyl coenzyme A oxidase, Tween-20, glycerinum, 1 to 1.5g / L of brij35-1 and ethylene diamine tetraacetic acid disodium salt and has the pH of 7.2. By adding different combinations of protein protecting agents, the enzyme activity stability is prolonged; the 37-DEG C stability of the enzyme is prolonged from 7 days to 10 days; the enzyme denaturation is slowed down; the reagent R2 cannot easily generate precipitate.

Description

technical field [0001] The invention relates to the technical field of free fatty acid measurement, in particular to a free fatty acid measurement kit, and also to a preparation method of the free fatty acid measurement kit. Background technique [0002] Free fatty acids (NFFA) refer to non-esterified fatty acids or unlipidated fatty acids. The metabolic activity of NEFA in serum is extremely high, and it is easily affected by fat metabolism, glucose metabolism and endocrine metabolism. With the deepening of research and the continuous improvement of technology, the relationship between serum NEFA and diseases has gradually become clear. NEFA has been confirmed to be associated with the occurrence and development of cardiovascular and cerebrovascular, respiratory, digestive, endocrine, immune and other system diseases, as well as tumors and traumatic stress. are closely related to energy metabolism. It is an important substance that induces insulin resistance, and it is a ...

AI Technical Summary

Problems solved by technology

[0005] 1) Reagent R2 containing peroxidase and acyl-CoA oxidase has a complex composition and contains a variety of enzymes, whose stability is related to enzyme activity, and enzyme activity is easily interfered by various factors, such as pH, temperature, Ultraviolet rays, heavy metal salts, inhibitors, activators, etc. lead to lack of stability of the kit and short storage time, which affects clinical use;

[0006] 2) The amount of enzyme used in reagent R2 is large, and the reagent is prone to precipitation

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