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Preparation method of high-purity lactosucrose

A technology of lactulose-oligosaccharide and lactose, which is applied in the field of biological and high-purity lactulose-oligosaccharide preparation, can solve the problems of low purity of lactulose-oligosaccharide and failure to meet actual needs, and achieve increased product added value and high stability , The effect of reducing production costs

Active Publication Date: 2016-12-07
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Since the above-mentioned technical schemes all use Arthrobacter chlorophenes SK33.001 as the enzyme source of the enzymatic method, due to the limitation of the enzyme, the yield and the purity of oligosaccharides are low, which cannot meet the actual needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A strain of Arthrobacter sp. BLCY-004 was preserved on August 16, 2016 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, address: Microbiological Research, Chinese Academy of Sciences, No. 1, Yard 1, Beichen West Road, Chaoyang District, Beijing Institute, accession number CGMCC No.12855;

[0040] The original strain of Arthrobacter sp. BLCY-004 of the present invention was isolated from the soil near the production workshop of Bailong Chuangyuan, Dezhou, Shandong, and obtained after mutagenesis. The specific isolation process is as follows:

[0041] (1) Enrichment culture

[0042] Select the soil near the production workshop of Bailong Chuangyuan, Dezhou, Shandong, remove the topsoil with a small shovel, take about 10g of soil 5-15cm above the ground, dilute it 10 times with sterile water, add the medium for enrichment culture, and the composition of the medium : Glucose 2%, yeast extract 1.5%, potassium dihydrogen phospha...

Embodiment 2

[0054] A preparation method of lactulose oligosaccharide, the steps are as follows:

[0055] (1) Prepare a mixed solution of sucrose and lactose with a mass concentration of 20%, and the addition ratio of sucrose and lactose is 1.2:1; adjust the pH of the mixed solution to 4, inoculate the Arthrobacter fermentation broth in a ratio of 5% of the mixed solution volume, Under the condition of 50 DEG C, react for 12 hours, then add a sucrose solution with a mass concentration of 40% to the reaction solution, maintain the sucrose mass concentration in the reaction solution as 30%, stop the reaction after continuing the reaction for 12 hours, and obtain crude lactulose oligosaccharides liquid;

[0056] (2) The crude lactulose oligosaccharide solution prepared in step (1) is added to activated carbon in a ratio of 0.5% by mass, stirred at 80° C. for 40 min; then filtered through a plate and frame, with a filtration pressure of 0.4 Mpa and a water flow rate of 6.0 t / h; A continuous i...

Embodiment 3

[0066] A preparation method of lactulose oligosaccharide, the steps are as follows:

[0067] (1) prepare mass concentration and be the mixed solution of sucrose and lactose of 40%, the addition ratio of sucrose and lactose is 1.3:1; Regulate the pH of mixed solution to 5, inoculate Arthrobacter fermented liquid according to the ratio of mixed solution volume 5%, Under the condition of 55 DEG C, react for 12 hours, then add a sucrose solution with a mass concentration of 40% to the reaction solution, maintain the sucrose mass concentration in the reaction solution as 35%, stop the reaction after continuing the reaction for 18 hours, and obtain crude lactulose oligosaccharides. liquid;

[0068] (2) The crude lactulose oligosaccharide solution obtained in step (1) is added to activated carbon in a ratio of 0.25% by mass, stirred at 80° C. for 40 min; then filtered through plate and frame, with a filtration pressure of 0.2 Mpa and a water flow rate of 5.0 t / h; A continuous ion ex...

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Abstract

The invention relates to a preparation method of high-purity lactosucrose. The preparation method includes the steps of firstly, inoculating Arthrobacter fermentation liquor into the mixed solution of sucrose and lactose, and performing reaction to obtain crude lactosucrose liquid, wherein Arthrobacter is Arthrobacter sp. BLCY-004, the preservation number of the Arthrobacter sp. BLCY-004 is CGMCC No. 12855; secondly, performing decoloring, filtering, ion exchange, chromatographic separation, concentration and drying on the crude lactosucrose liquid to obtain the lactosucrose. The preparation method has the advantages that the fermentation liquor of the Arthrobacter sp. BLCY-004 is used to produce the lactosucrose, the activity of the fructose transfer base enzyme produced by the strain is increased by more than 50% as compared that of an enzyme produced by traditional Arthrobacter, the lactulose conversion ability of the sucrose is increased greatly, sucrose feeding is used to allow the lactosucrose content in the final product to reach 98%, and the lactosucrose content of the final product is evidently better than that of the product prepared by an existing method.

Description

technical field [0001] The invention relates to a preparation method of high-purity lactulose-oligosaccharide, which belongs to the technical field of biotechnology. Background technique [0002] Lactulooligosaccharide is a functional oligosaccharide, which has special physiological functions such as promoting the proliferation of intestinal bifidobacteria, regulating intestinal microecology, and improving intestinal immunity. The sweetness is 30% of sucrose, and the sweetness is similar to that of sucrose Compared with sucrose, the sweetness quality is the best among various oligosaccharides, so it can be used in the food industry without worrying about its impact on product flavor. Commercially produced lactulose-oligosaccharides are slightly sweeter because they contain other ingredients such as sucrose and lactose. Compared with other oligosaccharides, oligosaccharides have higher stability to acid and heat. Its stability is similar to that of sucrose. It is stable unde...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12R1/06
CPCC12P19/00
Inventor 李方华窦光朋干昭波邵先豹张明站杜倩
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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