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Preparation method for beautifying and anti-aging autologous adipose-derived stromal cells

A fat source and stem cell technology, applied in the biological field, can solve the problems of limited biological beauty masks

Inactive Publication Date: 2016-12-07
ANHUI HUIEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Now the bio-cosmetic masks on the market have very limited effects on skin beauty, limited to moisturizing, whitening and oil control.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing autologous adipose-derived stem cells for beauty and anti-aging. First, obtain 750 mg of normal subcutaneous fat tissue in the abdomen, wash it with PBS for 5 times, remove the drugs, oil and blood cells used in liposuction, and use ophthalmic scissors to break the fat tissue. For small blood vessels visible to the naked eye, after the blood is fully washed with PBS, the adipose tissue is shredded to a size of 2.5 mm, and digested with 0.1% type I collagenase at a mass volume ratio of 37°C for 55 minutes;

[0023] Secondly, after digestion, centrifuge at 1000 rpm for 9 minutes, absorb and discard the upper layer of fat, add 1.5*PBS to mix, and filter through a 220-mesh cell sieve; , 5% volume concentration of CO 2 Incubate in the incubator, and replace the medium with fresh medium every 48 hours. It can be seen that the cells grow from the tissue block, which is the primary cell;

[0024] Again, when the primary cells reach 62% confluence, remove...

Embodiment 2

[0027] A preparation method of autologous adipose-derived stem cells for beauty and anti-aging. Firstly, obtain 880 mg of normal subcutaneous fat tissue in the abdomen, wash it with 0.8*PBS for 4 times, remove the drugs, oil and blood cells used in liposuction, and cut the fat with ophthalmic scissors For the small blood vessels visible to the naked eye in the tissue, after the blood is fully washed with PBS, the adipose tissue is shredded to a size of 3mm, and digested with 0.1% type I collagenase at a mass volume ratio of 37°C for 60 minutes;

[0028] Secondly, after digestion, centrifuge at 1000 rpm for 10 minutes, absorb and discard the upper layer of fat, add PBS to mix, and filter through a 200-mesh cell sieve; % volume concentration of CO 2 Incubate in an incubator, and replace the medium twice with fresh medium every 48 hours. It can be seen that the cells grow from the tissue block, which is the primary cell;

[0029] Again, when the primary cells reach 61% confluenc...

Embodiment 3

[0032] A preparation method of autologous adipose-derived stem cells for beauty and anti-aging. First, obtain 1000 mg of normal subcutaneous fat tissue in the abdomen, wash it with 1.2*PBS for 5 times, remove the drugs, oil and blood cells used in liposuction, and cut the fat with ophthalmic scissors For the small blood vessels visible to the naked eye in the tissue, after the blood is fully washed with PBS, the adipose tissue is shredded to 1-3mm in size, and digested with 0.2% type I collagenase at a mass volume ratio of 37°C for 70 minutes;

[0033] Secondly, after digestion, centrifuge at 1000 rpm for 12 minutes, absorb and discard the upper layer of fat, add PBS to mix, and filter through a 200-mesh cell sieve; % volume concentration of CO 2 Incubate in the incubator, and replace the medium with fresh medium every 48 hours. It can be seen that the cells grow from the tissue block, which is the primary cell;

[0034]Again, when the primary cells reach 65% confluence, remo...

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PUM

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Abstract

The invention discloses a preparation method for beautifying and anti-aging autologous adipose-derived stromal cells. The method comprises the following steps: acquiring normal belly subcutaneous adipose tissue; washing with PBS; cutting adipose tissues; digesting with I-type collagenase for a period of time; after digesting, adding PBS, uniformly mixing and filtering; adding a DMEM culture solution containing human autoserum; putting into an incubator for culturing; exchanging liquid with fresh culture solution at a certain time interval; causing the cells grow from the tissue blocks to original autologous adipose-derived stromal cells; washing with PBS when the original autologous adipose-derived stromal cells reach a certain fusion degree, and then adding pancreatin and EDTA digestive juice; adding human autoserum and ending digestion after the cells suspend, and then adding normal saline; moving the cell suspension to a centrifugal tube for centrifuging; collecting adipose-derived stromal cells and adjusting cell density. According to the method provided by the invention, the beautifying and anti-aging autologous adipose-derived stromal cells are prepared in agarose through the acquired autologous adipose-derived stromal cells and autologous thrombocyte-rich plasma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of autologous adipose-derived stem cells for beauty and anti-aging. Background technique [0002] In recent years, the use of living cells for beauty has become a hot spot in the market, especially the use of stem cells. Through direct injection on wrinkles, scars and scars, living cells can differentiate and grow in the skin, filling and repairing the skin. . Most of these living cells are derived from homologous allogeneic cells, which will produce immune rejection when entering the body's own immune system, and the cosmetic effect produced is relatively low. Adipose stem cells are a new type of adult stem cells discovered in recent years. The cells have strong proliferative ability, can maintain stable growth and proliferation activity in vitro culture, and have multiple differentiation potentials. They have also been applied in cosmetic treatments. However, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K8/98A61Q19/08A61Q19/00A61Q19/02
CPCA61K8/981A61K8/983A61Q19/00A61Q19/02A61Q19/08C12N5/0667C12N2509/00
Inventor 张正亮
Owner ANHUI HUIEN BIOTECH
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