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Preparation method of gnaphalium affine total flavone

A technology of total flavonoids from sagebone and total flavonoids, which is applied in the field of preparation of total flavonoids from sagebrush, can solve the problems of complex process, large amount of organic solvent, long process, etc., achieve good separation effect, easy operation, and reduce waste

Active Publication Date: 2016-12-07
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Commonly used methods for extracting total flavonoids include water decoction, heat reflux extraction, ultrasonic-assisted extraction, microwave-assisted extraction, etc., followed by further enrichment and extraction through macroporous adsorption resins, etc. However, the existing extraction methods are more complicated. The process is long, and the effect of extracting flavonoids is relatively poor. Secondly, organic solvents are used in the prior art. The organic solvents used include methanol, ethanol acetate, acetone, etc. The amount of organic solvents used is large, and there are many types, which have a greater impact on the environment. and many more

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  • Preparation method of gnaphalium affine total flavone
  • Preparation method of gnaphalium affine total flavone
  • Preparation method of gnaphalium affine total flavone

Examples

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preparation example Construction

[0015] A preparation method of total flavonoids from Shamrock, comprising the steps of:

[0016] (1) Sage sage is used as raw material, and dried sage is naturally dried in the shade and then pulverized into powder of sage;

[0017] (2) Add the ethanol extractant with a concentration of 50% to 70% to the sagebrush powder, and immerse it for 30min to 60min, then take 0.5h to 1h of ultrasonic extraction or heating reflux extraction or cold immersion extraction, and then pass through the combined extracts in sequence , suction filtration, concentration under reduced pressure and vacuum drying to finally obtain the solid powder of sage flavone crude extract, the filter residue after the suction filtration is repeatedly extracted once, and then concentrated under reduced pressure and vacuum drying can also obtain sage flavone Crude extract solid powder;

[0018] (3) Add the solid powder of sage flavonoids crude extract to 50% ethanol aqueous solution to obtain a mixed solution, th...

Embodiment 1

[0021] like figure 1 As shown, 1) Extraction of total flavonoids from S. chinensis: Accurately weigh 1 kg of dried 100-mesh S. sageum powder, add a certain amount of 70% ethanol solution as the extractant to infiltrate for about 30 minutes according to the ratio of solid to liquid of 1:35, and place in ultrasonic The power is 250W, the extraction temperature is 35 ℃ for the ultrasonic equipment for extraction, the extraction time is 60min, and the number of extractions is twice, the two extractions are combined, and then filtered, reduced pressure concentration and vacuum drying to obtain 156g of Sage sage Crude flavone extract solid powder;

[0022]2) Separation and purification: Weigh 0.1 kg of the solid powder of the crude extract of sage flavonoids, and disperse it in 50% methanol aqueous solution at a ratio of 1:6, load it on a 0.5 kg stationary phase Zn ion chromatography for separation, and then sequentially Elution with pure water and elution with 1% hydrochloric acid...

Embodiment 2

[0024] like figure 2 As shown, 1) Extraction of total flavonoids from Sagebrush: Accurately weigh 0.5kg of dried 100-mesh Shamrock powder, add a certain amount of 75% ethanol solution as the extractant to infiltrate for about 60 minutes at a solid-liquid ratio of 1:15, and heat and reflux for extraction The duration is 60 minutes, the number of extractions is twice, the two extracts are combined, suction filtered, concentrated under reduced pressure, and vacuum-dried to obtain 103 g of sage flavonoids crude extract solid powder;

[0025] 2) Separation and purification: Weigh 50 g of the solid powder of the crude extract of sage flavonoids, and disperse it in 50% methanol aqueous solution at a ratio of 1:6, load it on a 0.2 kg stationary phase Cu ion chromatography for separation, and then sequentially Elution with pure water and elution with 1% hydrochloric acid-methanol, and then collect the eluate that was eluted with 1% hydrochloric acid-methanol, and the eluate was concen...

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Abstract

The invention discloses a preparation method of gnaphalium affine total flavone. The preparation method includes following steps: (1), smashing dry gnaphalium affine into gnaphalium affine powder; (2), adding the gnaphalium affine powder into an alcohol extractant of 50-70% in concentration, immersing for 30-60min, adopting ultrasonic extraction or heating reflux extraction for 0.5-1h, and sequentially performing extract mixing, suction filtration, depressurizing compression and vacuum drying to obtain gnaphalium affine flavone crude extract solid powder; (3), adding the gnaphalium affine flavone crude extract solid powder into 50% alcohol water solution to obtain mixed liquid, subjecting the mixed liquid sequentially to stationary phase metal ion chelation chromatographic column separation, pure water elution and elution with 1% hydrochloric acid-methanol, collecting eluate of elution with 1% hydrochloric acid-methanol, and subjecting the eluate sequentially to depressurizing compression and vacuum drying to obtain a gnaphalium affine flavone product. The preparation method is reasonable in design, simple, convenient and quick to operate, efficient, energy-saving and more environment-friendly.

Description

technical field [0001] The invention relates to the field of natural medicine plus technology, in particular to a preparation method of total flavonoids of sagebrush. Background technique [0002] Gnaphalium affine (Gnaphalium affine D.Don) is also known as Qingming grass, alias spinach grass, vermicelli, soft bird grass, soft gorgon, wormwood, wormwood, Qingming vegetable, water hagi, unintentional, unintentional grass, Tian AI , Pengcao, and glutinous rice green are plants of the family Asteraceae and the genus Sage. "Supplements to Materia Medica" claims that "S. syringus, born in the hills and cultivated land, more than a foot high, with white hairs on the leaves, and yellow flowers", has been included in the 1977 edition of "Pharmacopia of the People's Republic of China". Contains more flavonoids and diterpenoids, because flavonoids have anti-oxidation, anti-allergic, anti-inflammatory, anti-bacterial, anti-inflammatory, anti-mutation, anti-tumor, hepatoprotective effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07G99/00A61K36/28A23L33/105
CPCA61K36/28C07G99/00
Inventor 张宏建胡轶娟徐攀梁卫青周洁程林浦锦宝
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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