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Method for increasing fermentation unit of acarbose

A technology of acarbose and unit, which is applied in the field of medicine and can solve the problems of uncontrolled light duration and intensity and low fermentation unit

Active Publication Date: 2016-11-30
CSPC SHENGXUE GLUCOSE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional slant culture process, the whole process is generally protected from light or natural light. The duration and intensity of light are not controlled, and there are problems such as low fermentation units.

Method used

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  • Method for increasing fermentation unit of acarbose
  • Method for increasing fermentation unit of acarbose
  • Method for increasing fermentation unit of acarbose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 light condition test

[0016] (1) Seed bottle seed culture

[0017] Each cultured fresh slant was inoculated into a 500m Erlenmeyer flask with a seed medium loading of 20%, and was shaken on a shaker at a speed of 250r / min for 48 hours at a temperature of 27°C to obtain a shaker flask seed solution;

[0018] (2) Shake flask fermentation

[0019] Connect the cultured mother bottle seed solution to a 500m1 Erlenmeyer flask with a fermentation medium loading of 10% according to a 15% inoculum amount, and shake and cultivate at a speed of 250r / min for 168h at a temperature of 27°C;

[0020] See the experimental results figure 1 ,Depend on figure 1 It can be seen that during the cultivation of Actinoplanes sp. on the slant, incandescent lamps are used as the light source, the illumination time is controlled at 12-20h / day, and the illumination is 120-180lux, and the best is 12-16h / day, and the illumination is 150-180lux.

Embodiment 2

[0021] Embodiment 2 comparative experiment

[0022] Prepare slant medium, and its substratum is composed as follows: sucrose 30g / L, peptone 5g / L, KC10.5g / L, KH2P041.0g / L, L-tyrosine 1g / L, MgS040.5g / L, agar 20g / L, the solvent is water, and the initial pH is 7.0. Sterilize at 121°C for 30 minutes.

[0023] Prepare seed bottle culture medium, the culture medium composition is as follows: starch 10g / L, soybean cake powder 10g / L, CaCO 3 2g / L, glycerin 20g / L, solvent is water, initial pH is 7.0. Sterilize at 121°C for 30 minutes.

[0024] Prepare fermentation shake flask culture medium, the culture medium composition is as follows: maltose 60g / L, glucose 20g / L, soybean cake powder 15g / L, FeCl 3 0.2g / L, CaCl 2 2g / L, CaCO 3 4g / L, sodium glutamate 2g / L, KH2P04 1g / L, the solvent is water and the initial pH is 7.0. Sterilize at 121°C for 30 minutes.

[0025] Slant strain preparation: Aseptically transfer the low-temperature glycerol tube strain to fresh, sterile slant medium. On...

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Abstract

The invention relates to a method for increasing the fermentation unit of acarbose. The fermentation unit is increased through control of slant strain cultivation conditions of Actinoplanes sp.. Experimental results show that incandescent lamps serve as light sources during slant cultivation of the Actinoplanes sp.; when illumination time is controlled within 12-20h / day and illuminance is controlled within 120-180lux, the fermentation unit can be increased; particularly, when the illumination time is controlled within 12-16h / day and the illuminance is controlled within 150-180lux, the fermentation unit is increased remarkably.

Description

technical field [0001] The invention relates to a method for increasing the fermentation unit, in particular to a method for increasing the fermentation unit of acarbose, which belongs to the technical field of medicine. Background technique [0002] Acarbose is a pseudotetrasaccharide substance fermented by Actinoplanessp, Chinese alias: O-4,6-dideoxy-4[[(1S,4R,5S,6S)4,5 , 6-trihydroxy-3-(hydroxymethyl)-2-cyclohexene]amino]-(-D-glucopyranosyl (1→4)-O-)-D-glucopyranosyl ( 1 → 4) -D-glucopyranose. Acarbose can compete with α-glucosidase (Wehmeier UF, Piepersberg W. Biotechnology and molecular biology of the α-glucisidase inhibitoracarbose [J]. Appl Microbiol Biotechnol, 2004, 63: 613-625) to inhibit food Decomposition of polysaccharides slows down the absorption of sugar accordingly, thereby reducing postprandial hyperglycemia, and treating diabetes with diet. [0003] The metabolic pathways of microorganisms are coordinated by many related metabolic pathways, and their me...

Claims

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Application Information

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IPC IPC(8): C12P19/26C12R1/045
CPCC12P19/26
Inventor 刘军旗王俊刚李英然何茹曹艳霞赵宇李珍贾伟娜刘汉忠李雪然袁玉伟张敬坤马蕙宋娅娅张蕾
Owner CSPC SHENGXUE GLUCOSE CO LTD
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