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Preparation method of marine active calcium containing beneficial bacteria

A technology of beneficial bacteria and active calcium, which is applied in the field of preparation of marine active calcium, can solve the problems of ineffective use and destruction, and achieve the effect of improving the body's immunity and enriching active calcium components

Inactive Publication Date: 2016-11-30
NINGBO JIANGDONG HUIZE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these beneficial substances are completely oxidized and destroyed during high-temperature calcination and cannot be effectively utilized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of the marine active calcium that contains beneficial bacteria of the present embodiment comprises the following steps:

[0027] (1) By weight, get 0.02 part of corn flour, 0.02 part of bean flour, 0.01 part of NAMn medium, 5 parts of fineness of 80 orders, 40% solid content of snail shell slurry, 2 parts of fineness of 80 mesh, cyanophyta algae slurry with a solid content of 20% and 100 parts of water, mixed evenly for later use;

[0028] (2) Add the mixture obtained in step (1) into a fermenter, seal it tightly, and feed in steam sterilized by filtration, maintain the temperature at 121-123° C., and sterilize under pressure at 0.11 MPa for 0.5 hours;

[0029] (3) Cool down to below 30°C, insert Bacillus subtilis at a ratio of 3% of the inoculum, heat up to 30°C for liquid-phase fermentation, during the fermentation process, detect the fermentation situation, and stop the fermentation when the spore rate reaches more than 90%;

[0030] (4) Dryin...

Embodiment 2

[0033] The preparation method of the marine active calcium that contains beneficial bacteria of the present embodiment comprises the following steps:

[0034] (1) By weight, get 0.5 part of corn flour, 0.3 part of bean flour, 0.05 part of NAMn medium, 25 parts of chiton shell slurry whose fineness is 100 mesh, solid content of 60%, 20 parts of fineness Protochlorophyta algae slurry of 100 mesh, 60% solid content and 100 parts of water, mixed evenly for later use;

[0035] (2) Add the mixture obtained in step (1) into the fermenter, seal it tightly, and feed it with filtered and sterilized steam, maintain the temperature at 122-124° C., and sterilize under pressure at 0.11 MPa for 2 hours;

[0036] (3) Cooling down to below 30°C, inserting Bacillus licheniformis in proportion to 10% of the inoculum amount, raising the temperature to 45°C for liquid-phase fermentation, during the fermentation process, detecting the fermentation situation, and stopping the fermentation when the s...

Embodiment 3

[0039] Embodiment 3: the preparation method of the marine active calcium containing beneficial bacteria of the present embodiment comprises the following steps:

[0040] (1) By weight, get 0.1 part of corn flour, 0.3 part of bean flour, 0.03 part of NAMn medium, 10 parts of fineness of 80 mesh, 50% solid content of oyster shell slurry, 15 parts of fineness of 100 mesh, Rhodophyta algae slurry with a solid content of 40% and 100 parts of water, mixed evenly for subsequent use;

[0041] (2) Add the mixture obtained in step (1) into the fermenter, seal it tightly, feed in steam sterilized by filtration, maintain the temperature at 123-125°C, and sterilize under pressure at 0.11MPa for 1 hour;

[0042](3) Cooling down to below 30°C, inserting Bacillus subtilis and Bacillus licheniformis with a quality of 1:1 at a ratio of 5% of the inoculum amount, raising the temperature to 40°C for liquid phase fermentation, during the fermentation process, detecting the fermentation situation, ...

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PUM

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Abstract

The invention relates to a preparation method of marine active calcium containing beneficial bacteria. According to the method, starch, a NAMn culture medium, shellfish and seaweed marine plants are mixed and undergo high-temperature disinfection and sterilization, the mixture is inoculated with beneficial bacteria, aerobic liquid-phase fermentation is carried out to convert calcium in the shellfish powder to marine active calcium while seaweed marine plants are degraded into active materials such as alginic acid, glucosamine oligosaccharide, organic polypeptide, amino acid and the like with the fermentation process and various medium and trace elements enriched in marine organisms and shells are released; and finally, the product is dried to obtain the marine active calcium containing beneficial bacteria. According to the method, without high-temperature calcination, natural organic active calcium and substances with effects of resisting virus, resisting bacteria and enhancing body immunity in shellfish marine species are maintained to the maximum. Through the liquid-phase fermentation technology, macromolecular organic matter or high-molecular substances in shellfish marine species are degraded into micromolecules, which are beneficial to digestion and absorption in animals or plants.

Description

technical field [0001] The invention relates to the technical field of preparation of active calcium, in particular to a preparation method of marine active calcium containing beneficial bacteria. Background technique [0002] Biological microecological preparations are a type of biological products that prevent and treat intestinal diseases of animals, induce plant resistance and reduce disease occurrence, and have been widely used in livestock and poultry breeding and plant pest control. Calcium deficiency in animals and plants is also one of the important factors affecting the growth of animals and plants. When calcium is deficient, cell division cannot be carried out or completed normally. For example, postpartum paralysis caused by calcium deficiency in dairy cows, calcium-deficient diet, etc. will cause bone calcium loss , bone and joint damage, etc.; when the plant is deficient in calcium, the front end of the root will turn brown, the branches and leaves will become ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K20/24A23K20/147A23K20/142A23K20/121A23K20/163A23K20/20C05D5/00C05G3/00C05G3/04C12P1/04C12R1/125C12R1/10C05G3/80
CPCC05D5/00C05G3/00C12P1/04C05G3/80C12N1/205C12R2001/10C12R2001/125
Inventor 孟秋峰韩慧聪潘龙琳
Owner NINGBO JIANGDONG HUIZE BIOLOGICAL TECH CO LTD
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