Production method of microbial antioxidant
An antioxidant and production method technology, applied in the directions of antitoxins, drug combinations, pharmaceutical formulations, etc., can solve the problems of no outstanding novelty in the process, and achieve the effects of preventing Alzheimer's disease, improving natural healing power, and promoting development.
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Embodiment 1
[0028] Step 1: Cultivate Rhodopseudomonas palustris, Saccharomyces cerevisiae and Lactobacillus plantarum in the same medium at 25°C for 30 hours; the medium is composed of the following raw materials in weight percentage: Ganoderma lucidum spore powder 0.04%, Gynostemma pentaphyllum 0.1%, roxburghii 0.3%, wild honey 1%, kelp 2.5%, rice bran bean cake powder 4%, sterile water 92.06%;
[0029] Step 2: Inoculate the three bacterial strains obtained in the first step into the broth culture medium at the same time, culture at 35°C for 22 hours, take the culture solution for streak separation, and separate the isolated strains in the meat Carry out pure seed culture at 25°C for 30 hours in soup medium, and save as production strain;
[0030] Step 3: Transfer the production strains obtained in the second step to eggplant bottles filled with nutrient agar medium, culture them at 35°C for 45 hours, and then store them in a refrigerator at 2°C ;
[0031] The fourth step: Inoculate th...
Embodiment 2
[0037] Step 1: Cultivate Rhodopseudomonas palustris, Saccharomyces cerevisiae and Lactobacillus plantarum in the same medium at 30°C for 28 hours; the medium is composed of the following raw materials in weight percentage: Ganoderma lucidum spore powder 0.02%, Gynostemma pentaphyllum 0.12%, roxburghii 0.4%, wild honey 1.3%, kelp 3.5%, rice bran bean cake powder 3%, sterile water 91.66%, pH value adjusted to 6.0, temperature 121 ℃, steam sterilization for 30 minutes;
[0038] Step 2: Inoculate the three bacterial strains obtained in the first step into the broth culture medium at the same time, culture at 40°C for 26 hours, take the culture solution for streak separation, and separate the isolated strains in the meat Carry out pure seed culture in soup medium at 30°C for 28 hours, and save it as the production strain;
[0039] Step 3: Transfer the production strains obtained in the second step to eggplant bottles filled with nutrient agar medium, culture them at 40°C for 50 hou...
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