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A kind of phenylboronic acid functionalized magnetic nanoparticle with protective group and its preparation method and application

A magnetic nanoparticle and protective group technology, applied in chemical instruments and methods, magnetic objects, magnetic materials, etc., can solve the problems that cannot analyze low-abundance pure sugar samples, affect the purification analysis and research of pure sugar substances, etc. , to achieve the effect of fast and sensitive analysis

Inactive Publication Date: 2019-03-08
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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Problems solved by technology

However, since sugars generally coexist with proteins or polypeptides, during the purification process of pure sugars, proteins or peptides will largely affect the purification analysis and research of pure sugars
Therefore, the existing methods are still unable to quickly and sensitively analyze low-abundance pure sugar samples effectively

Method used

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  • A kind of phenylboronic acid functionalized magnetic nanoparticle with protective group and its preparation method and application
  • A kind of phenylboronic acid functionalized magnetic nanoparticle with protective group and its preparation method and application
  • A kind of phenylboronic acid functionalized magnetic nanoparticle with protective group and its preparation method and application

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preparation example Construction

[0031] In the second aspect, the present invention also provides a method for preparing phenylboronic acid functionalized magnetic nanoparticles with protective groups, wherein the method comprises:

[0032] (1) contacting magnetic nanoparticles with tetraethyl orthosilicate to obtain magnetic nanoparticles doped with silicon dioxide;

[0033] (2) contacting the magnetic nanoparticles doped with silicon dioxide with a silane coupling agent with active groups to obtain magnetic nanoparticles doped with silicon dioxide whose surface is modified with active groups;

[0034] (3) contacting the silica-doped magnetic nanoparticles whose surface is modified with an active group with a phenylboronic acid derivative and a protective group to obtain phenylboronic acid functionalized magnetic nanoparticles with a protective group; Wherein, the protecting group is a protecting molecule with a hydroxyl group at the end.

[0035] According to the present invention, the selection of the mag...

preparation example 1

[0056] Preparation of Superparamagnetic Fe3O4 Magnetic Nanoparticles

[0057] In the 250mL there-necked flask, add 25mL of 2M hydrochloric acid solution, then put 2g of ferrous chloride tetrahydrate (FeCl 2 4H 2 O) and 5.4g ferric chloride hexahydrate (FeCl 3 ·6H 2 O) the mixed sample joins in the hydrochloric acid, and feeds nitrogen to carry out protection. The reaction system was vigorously stirred at room temperature, and the FeCl 2 4H 2 O and FeCl 3 ·6H 2 After the O was completely dissolved, 30 mL of ammonia solution (28 to 30% by weight) was added dropwise to the reaction system. After the addition was complete, the reaction system was continued to be vigorously stirred at room temperature for 30 minutes to obtain a magnetic nanoparticles. The prepared superparamagnetic ferriferroferric oxide magnetic nanoparticles were washed three times with 50 mL of triple distilled water, and finally stored in 50 mL of degassed triple distilled water with a concentration of ...

Embodiment 1

[0066] Preparation of phenylboronic acid functionalized magnetic nanoparticles with protective group (2-(2-aminoethoxy)ethanol)

[0067] (1) 5 mL of the superparamagnetic iron tetroxide magnetic particle solution obtained in the preparation example was resuspended in 80 mL of ethanol, placed in a 250 mL three-necked flask, and ultrasonicated for 10 minutes to completely disperse it. Then, 8 mL of ammonia solution, 7.5 mL of triple distilled water and 0.1 mL of tetraethyl orthosilicate (TEOS) were successively added, and stirred vigorously at 40°C for 4 h to obtain a superparamagnetic Fe3O4 magnetic nanoparticles. The obtained superparamagnetic triferromagnetic nanoparticles doped with silicon dioxide were washed three times with 50 mL of triple distilled water to remove materials not doped with magnetic iron. Finally, the resulting particles were suspended in 50 mL of ethanol.

[0068] (2) Take 20mL of the superparamagnetic iron tetroxide magnetic nanoparticle solution doped...

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Abstract

The invention discloses a phenylboronic acid functionalized magnetic nanoparticle with a protecting group and a preparation method and application of the phenylboronic acid functionalized magnetic nanoparticle. The phenylboronic acid functionalized magnetic nanoparticle with the protecting group comprises a magnetic nanoparticle which is modified by an active functional group on the surface and is doped with silicon dioxide, a phenylboronic acid derivative modified on the surface of the magnetic nanoparticle and a protective molecule with hydroxyl in a terminal. The phenylboronic acid functionalized magnetic nanoparticle with the protecting group modified on the surface can specifically adsorb saccharides; nonspecific adsorption of other interferents can be eliminated; nonspecific interferents of protein, polypeptide and the like are effectively eliminated; and low-abundance saccharide samples are quickly and sensitively analyzed.

Description

technical field [0001] The present invention relates to phenylboronic acid functionalized magnetic nanoparticles with protective groups and a preparation method thereof, and the application of the phenylboronic acid functionalized magnetic nanoparticles with protective groups in specific adsorption of sugar substances, especially Application in purification and analysis methods of sugar substances. Background technique [0002] Glycosylation is one of the most common and important post-translational modification processes in eukaryotic cells, which plays an important role in various functions of organisms. More and more evidence shows that protein glycosylation and abnormal expression of carbohydrates are closely related to various diseases and the correct function of proteins, such as inflammation, immune diseases, tumors and metabolic syndrome. In addition, glucose and polysaccharides are also important energy sources for humans and animals. Therefore, the analysis of ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01F1/00B01J20/22B01J20/28B01J20/30B01D15/08
Inventor 梁兴杰薛向东
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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