Multiple myeloma (MM) prognosis related gene mutation detection kit and detection method

A multiple myeloma detection kit technology, applied in the fields of medicine and molecular biology, can solve the problems of cumbersome process and high rate of false detection and missed detection, and achieve the effect of simple detection process, good safety and high accuracy

Pending Publication Date: 2016-11-23
北京海思特医学检验实验室有限公司
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Problems solved by technology

[0007] Aiming at the current problems of high misdetection rate and cumbersome process of detection related to MM prognosis evaluation, the present invention provides a new MM prognosis related gene mutation detection technology, comprehensively applies PCR amplification and gene sequencing technology, and selects from MM highly mutated genes Twenty-two genes have developed a set of related gene mutation detection kits and detection methods for MM-specific prognosis assessment, which can quickly and comprehensively obtain data on the detection of MM prognosis-related gene mutations

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  • Multiple myeloma (MM) prognosis related gene mutation detection kit and detection method
  • Multiple myeloma (MM) prognosis related gene mutation detection kit and detection method
  • Multiple myeloma (MM) prognosis related gene mutation detection kit and detection method

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[0065] As a preferred embodiment, the detection genes may also include thirteen auxiliary detection genes: PIK3CA, ZFHX4, VCAN, PRDM1, CCND1, TRAF3, SPEN, ANK2, EGR1, FGFR3, NFKB2, NEB and SP140, the genes of which The source of the sequence is also the NCBI gene bank, and the specific identification is shown in Table 2 below:

[0066]

[0067] In the above table, the function of each gene is as follows:

[0068] PIK3CA gene mutations are often accompanied by KRAS mutations, especially G12C site mutations. The PI3K signaling pathway is very important because it can regulate downstream signaling pathways, thereby promoting cell proliferation, growth and metabolism;

[0069] The ZFHX4 gene belongs to the DNA damage repair signaling pathway, and its mutation is associated with poor prognosis;

[0070] The VCAN gene protein belongs to the ggrecan / versican proteoglycan family and is associated with tissue morphogenesis and maintenance;

[0071] The PRDM1 gene is a transcriptio...

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Abstract

The embodiment of the invention provides a multiple myeloma (MM) prognosis related gene mutation detection kit and a detection method. The kit comprises multiple pairs of specific primers corresponding to the detected genes respectively; and 22 genes are selected from MM prognosis related high-mutation genes as the detection gens; according to the invention, two technologies of PCR and sequencing are comprehensively applied, rapid detection is realized for the mutation conditions of the MM prognosis related genes, specific exon sections containing hotspot mutation in the 22 detected genes are amplified through corresponding specific primers, and the mutation result is obtained through sequencing and comparison with a standard gene sequence. According to the technical scheme provided by the invention, the related genes are comprehensively selected according to the pathogenesis of MM and related signal path, and the applicability is strong; the detection specificity is high, the detection process is simple and accurate, the result is exact and clear, and the purposefulness is strong; a few experimental devices are used, and the cost is saved; and moreover, without radiolabelling, the safety is good.

Description

technical field [0001] The invention belongs to the technical field of medicine and molecular biology, and in particular relates to a multiple myeloma prognosis-related gene mutation detection kit. The invention also provides a method for using the kit to detect multiple myeloma prognosis-related gene mutations. Background technique [0002] Multiple myeloma (Multiple Myeloma, MM) is a malignant plasma cell disease, and its tumor cells originate from plasma cells in the bone marrow, and plasma cells are cells that develop into the final functional stage of B lymphocytes, so multiple myeloma can Classified as B lymphocytic lymphoma. [0003] Traditional MM routine detection includes screening tests, diagnostic tests, assessment of tumor burden and prognosis tests, mainly through the detection of abnormal protein types and concentrations in blood for diagnosis, such as assessment of tumor burden and prognosis tests usually include: 1. Bone marrow smear 2. Serum β2 microglobu...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q1/6869C12Q2600/118C12Q2600/156C12Q2531/113
Inventor 傅廷娇陈欣张娟李悠南程宇王绪华梁超陈忠黄士昂
Owner 北京海思特医学检验实验室有限公司
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