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Dialysis device and micro-universe experimental device including same

A technology of a dialysis device and a dialysis bag, applied in the field of microcosmic experimental devices, can solve the problems of maintaining suspension, inability to sterilize at high temperature, difficulty in sampling the device, etc., and achieve the effects of easy and simple replacement, increased water exchange area, and small device system.

Inactive Publication Date: 2016-11-23
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The dialysis device in the dialysis bag microcosm device commonly used in the prior art is mostly a section of dialysis bag, and the two ends are sealed with sealing clips. It is difficult to take samples from such a device, and because the sealing clips are mostly plastic products, it is not only difficult to sample in water bodies. remain suspended in the medium and cannot be autoclaved

Method used

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  • Dialysis device and micro-universe experimental device including same
  • Dialysis device and micro-universe experimental device including same
  • Dialysis device and micro-universe experimental device including same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Such as figure 1 As shown, a dialysis device 2 includes a dialysis bag 21 and a bottom-removed centrifuge tube 22, 23, and the bottom-removed centrifuge tube 22, 23 includes a tube cover 31 and a tube body thread 30; the dialysis bag 2 is expanded to form a cylinder The two ends of the cylindrical dialysis bag 2 are respectively sealed by the tube caps 31 and the tube body threads 30 of the two bottomed centrifuge tubes 22 and 23.

[0031] In this embodiment, the bottom-removed centrifuge tubes 22 and 23 are made of 50ml centrifuge tubes with the bottom removed; the dialysis bag 2 has a molecular weight cut-off of 8000-14000, a width of 55mm, a diameter of 3.5mm after stretching into a cylindrical shape, and a bag length of 15cm.

Embodiment 2

[0033] Such as figure 2 As shown, a microcosm dialysis bag experimental device for studying the relationship between species in water ecosystems includes a dialysis device 2 and a culture container 1, and the dialysis device 2 is placed in the culture container 1; the dialysis device 2 includes a dialysis device Bag 21 and bottom-removed centrifuge tubes 22, 23. The bottom-removed centrifuge tubes 22, 23 include tube caps 31 and pipe body threads 30; The tube cover 31 and tube body thread 30 of two bottom-removed centrifuge tubes 22, 23 are sealed;

[0034] The liquid level of the solution in the dialysis device 2 is lower than the upper centrifuge nozzle 22, and the dialysis device is kept upright in the solution in the culture container.

[0035] The dialysis device 2 is provided with glass beads 24 so that the dialysis device 2 sinks to the bottom of the culture container 1 .

[0036] In this embodiment, the bottom-removed centrifuge tubes 22 and 23 are made of 50ml cent...

Embodiment 3

[0039] This example illustrates the preparation method of the dialysis device described in Example 1.

[0040] Such as image 3 As shown, remove the bottom of the two centrifuge tubes, retain the cap and the threaded part of the tube body; stretch the dialysis bag into a cylindrical shape, wrap a centrifuge tube body with the bottom removed at both ends of the dialysis bag, and wrap the two ends of the dialysis bag The mouth is folded inward, and the cap of the centrifuge tube is tightened to form a closed system; that is, the dialysis device.

[0041] When applied to the microcosm experimental device, place a load in the bottom-bottomed centrifuge tube as needed.

[0042] The bottom-removed centrifuge tube is made of a 50ml centrifuge tube with the bottom removed; the specification of the dialysis bag is molecular weight cut-off 8000-14000, width 55mm, diameter 3.5mm after stretching into a cylinder, and bag length 15cm.

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Abstract

The invention discloses a dialysis device and a micro-universe experimental device including the dialysis device. The dialysis device comprises a dialysis bag and bottom-off centrifugal tubes, wherein each bottom-off centrifugal tube comprises a tube cap and a tube thread; the dialysis bag is distended in a cylinder body, and both ends of the cylindrical dialysis bag are sealed respectively through the tube caps and the tube threads of the bottom-off centrifugal tubes. The micro-universe experimental device includes the dialysis device and a culture container, and the dialysis device is placed in the culture container. The device is used to study the indirect effects of interspecific relationships in aquatic ecosystems and can be applied to scientific experiments and special cultivation of plankton. The dialysis device and the micro-universe experimental device are conducive to conducting relevant researches with scientific value and social benefits.

Description

technical field [0001] The invention belongs to the field of biotechnology, specifically a microcosm experimental device that uses a dialysis bag to intercept macromolecular substances, penetrate small molecular substances, thereby blocking the direct contact of organisms, and researching the relationship between species through the transmission of information substances. . Background technique [0002] The interspecific relationship of organisms is the mutual relationship between individual species in a biome. The organisms in the community are not independent individuals, but are generally connected by non-biological factors such as energy and material resources and habitats. The interspecies relationship of organisms is very complex, mainly including primitive cooperation, symbiosis, symbiosis, parasitism, predation, competition and other types. Organisms occupying different trophic levels in the biome transfer energy and exchange information through substances, signals...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00A01K67/033
CPCA01K67/033C12Q1/02
Inventor 杨州朱雪霞王骏张露谷磊
Owner NANJING NORMAL UNIVERSITY
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