Method for producing astaxanthin

A production method, technology of astaxanthin, is applied to light irradiation. field, which can solve problems such as inability to judge astaxanthin production, failure to mention astaxanthin production stage, and improvement

Inactive Publication Date: 2016-11-16
NIPPON SUISAN KAISHA LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no mention of the astaxanthin production stage after encapsulation, nor is it documented whether astaxanthin can be produced
Since it is known that Haematococcus undergoes encapsulation due to stress during growth and accumulates a large amount of astaxanthin, it is not possible to judge whether astaxanthin production is improved in this literature

Method used

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  • Method for producing astaxanthin
  • Method for producing astaxanthin
  • Method for producing astaxanthin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Culture of Haematococcus (proliferation culture)

[0080] 15 ml of culture solution of Haematococcus lacustrine (H. lacustris) NIES144 strain (preserved by the Microorganism System Preservation Facility of the National Institute of Environmental Research) and BG11 modified A medium (Table 1) containing planktonic cells with a cell number of 500,000 ) 750 ml into four transparent culture containers made of glass with an inner diameter of 50 mm and a height of 500 mm. The luminous flux density becomes 50μmol / m 2 Continuous light irradiation with a fluorescent lamp was carried out in a / s mode, and the air containing 1% carbon dioxide was passed through at 25° C., and cultured while stirring. As a result, the proliferation of planktonic cells was confirmed to be 450,000 / ml on the fifth day of culture.

[0081] Cultivation of Haematococcus using various light sources (astaxanthin production culture)

[0082] Next, after adding sodium chloride to the culture solution so t...

Embodiment 2

[0092] Culture of Haematococcus (proliferation culture)

[0093] Inject 15 ml of culture solution of Haematococcus lacustrine (H. lacustris) NIES 144 strain containing 500,000 cells / ml of planktonic cells and 750 ml of BG11 modified B medium (Table 3) into a glass with an inner diameter of 50 mm and a height of 500 mm. in a transparent culture container. By using a blue LED with a wavelength of 450nm (luminous flux density 50μmol / m 2 / s), red light with a wavelength of 660nm (luminous flux density LED 30μmol / m 2 / s) while being continuously irradiated with light, at 25° C., air containing 1% carbon dioxide was blown, and cultured while stirring. As a result, proliferation of 360,000 planktonic cells / ml was confirmed on the fourth day of culture.

[0094] Cultivation of Haematococcus (Astaxanthin Production Cultivation)

[0095] Next, after adding sodium chloride to the culture solution so that the concentration of sodium chloride becomes 2g / L, by using blue light with a wa...

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Abstract

A method which heightens the efficiency of production of astaxanthin by the cultivation of a microalga. The method for producing astaxanthin comprises incubating a microalga to cause the microalga to produce astaxanthin in the alga cells, and is characterized in that light irradiation during at least the astaxanthin production cultivation period in the cultivation period is conducted using blue LEDs having a peak wavelength of 420-500 nm and red LEDs having a peak wavelength of 620-690 nm in combination. The ratio of the blue LEDs having a peak wavelength of 420-500 nm to the red LEDs having a peak wavelength of 620-690 nm is preferably 1:19 to 19:1 in terms of photon flux density. The photon flux density of each of the LEDs is preferably 20 [mu]mol / m2 / s or greater.

Description

technical field [0001] The present invention relates to an efficient production method of astaxanthin. More specifically, it relates to light irradiation when culturing astaxanthin-producing microalgae. Background technique [0002] Astaxanthin is one of the orange-red carotenoids, and it is a pigment mainly contained in large amounts in crustaceans such as shrimps and crabs, salmon, salmon roe, sea bream, and algae. This astaxanthin is known to have a strong antioxidant effect, and is used as a food coloring, cosmetics, health food, pharmaceuticals, and the like. [0003] Astaxanthin is produced by chemical synthesis or by culturing bacteria, yeast, microalgae, etc. The content of astaxanthin per unit dry weight of bacteria and yeast is 2% by weight or less. On the other hand, among microalgae, microalgae of the genus Haematococcus (hereinafter referred to as Haematococcus) are cultivated. The obtained astaxanthin is produced worldwide because it can be cultured at a hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12N1/12C12R1/89
CPCC12N1/12C12N13/00C12P23/00C12R2001/89C12N1/125
Inventor 泉田仁大桥英治沼沢彻
Owner NIPPON SUISAN KAISHA LTD
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