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A novel saccharomycopsis fibuligera strain, a culturing method thereof and uses of the strain

A technology of buttoning capsules, laminating membranes and culturing methods, which is applied in the field of microorganisms and achieves the effect of great application value

Active Publication Date: 2016-11-09
四川伯禹生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of strains with good ability to remove glucosinolate and gossypol at the same time

Method used

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  • A novel saccharomycopsis fibuligera strain, a culturing method thereof and uses of the strain
  • A novel saccharomycopsis fibuligera strain, a culturing method thereof and uses of the strain
  • A novel saccharomycopsis fibuligera strain, a culturing method thereof and uses of the strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The screening of embodiment 1 bacterial strain

[0039] In the process of researching traditional liquor brewing, the present invention screens and obtains some bacterial strains from distillery waste. After further research, some strains have high biomass in culture, so they grow well when they are cultured on various agricultural by-products (including various processed residues such as discarded grains) as substrates. It was further found that a strain of bacteria had the effect of reducing the content of toxic substances on the cake raw materials. Name it WNY-2 and conduct further research.

Embodiment 2

[0040] Identification of Example 2 Bacterial Strains——16S rDNA Sequence Analysis

[0041] (1) Total DNA extraction

[0042] After culturing with potato sucrose medium, the cells were collected by centrifugation, washed 3 times with sterile distilled water, dissolved in extraction buffer (100 mM Tris Cl, 100 mM EDTA-Na2, 200 mM NaCl, 2% CTAB, pH 8.0), 37 °C Shake for 45 minutes, add 20% SDS, and bathe in water at 65°C for 1 hour. Centrifuge at 12,000rpm for 10min, and collect the supernatant. The supernatant was extracted twice with an equal volume of phenol: chloroform: isoamyl alcohol (25:24:1), added with a final concentration of 0.3M NaAC (pH 5.2) and 2 times the volume of absolute ethanol, and precipitated at room temperature for 1 h . Centrifuge (12,000 rpm) at 4°C for 20 min. The precipitate was washed twice with 70% ethanol, evaporated to dryness and dissolved in 50 μL TE (10 mmTris-Hcl, 1 mmNa2EDTA).

[0043] (2) 16S rDNA amplification and sequencing

[0044] Usi...

Embodiment 3

[0049] Embodiment 3 Morphological characteristics and physiological and biochemical characteristics identification of bacterial strain CGMCC No.12221 of the present invention

[0050] 1. Observation of colony and bacterial morphology

[0051] Streak inoculate this strain on the potato sucrose plate medium, culture at 30°C for 4 days, observe the colony morphology during the culture (see image 3 ); pick a little thalline with an inoculation needle, make slices by soaking in water, and observe the shape of thalli under a microscope (see figure 1 and figure 2 ).

[0052] The results were as follows: under the microscope, the cells were oval, with a length of 5-7.5 μm (average 6.2 μm) and a width of 3.8-5 μm (4.5 μm). Visible cell budding, unilateral budding reproduction. Early growth forms hyphae and produces spores (see figure 1 and figure 2 )

[0053] Colony characteristics:

[0054] The colony is white and flat; the diameter is about 5-10 mm; the surface is rough an...

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Abstract

The invention belongs to the field of microbes and particularly relates to a novel saccharomycopsis fibuligera strain, a culturing method thereof and uses of the strain. A technical problem to be solved is to provide a technical means having good glucosinolate removing and gossypol removing capabilities. A technical scheme to solve the problem is to provide the novel saccharomycopsis fibuligera strain. The accession number of the novel saccharomycopsis fibuligera strain is CGMCC No.12221. The novel saccharomycopsis fibuligera strain is named as saccharomycopsis fibuligera number WNY-2. The strain has high detoxification capabilities for glucosinolate in rapeseed meal and gossypol in cottonseed meal. Tests prove that the strain is safe and nontoxic in oral administration. The strain provides a strain resource for mixed meal microorganism technical treatment, provides a reliable basis for large-scale preparation of a novel biological protein raw material and has extremely high application value in the fields of feed and cultivation.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a new bacterial strain of Saccharomycopsis fibuligera and a culture method and application thereof. Background technique: [0002] At present, there are a large number of agricultural by-products and processing by-products in my country, which are either discarded or not effectively utilized, such as rapeseed meal and cotton meal. It has high content and rich nutrients, but because it contains more toxic substances and anti-nutritional factors, its utilization rate in feed is extremely low. The main toxicant of rapeseed meal is glucosinolate (glucoside for short), and the main toxicant of cottonseed meal is gossypol. The amount of untreated rapeseed meal and cotton meal can only reach 3-5% in livestock and poultry feed (young livestock and poultry cannot be added), excessive use will cause damage to the organs of livestock and poultry, and reduce growth and reproductive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/04A23K10/12C12R1/645
CPCC12N1/04C12N1/16C12N1/145C12R2001/645
Inventor 邓小晨吴谦
Owner 四川伯禹生物工程有限公司
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