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Application of rhizoma smilacis glabrae extract for preventing and treating radish black rot

A Smilax smilax extract and technology of Smilax smilax, applied in the field of application of Smilax smilax extract in preventing and treating radish black rot, can solve the problems of complex preparation process, waste of resources, high cost, etc., achieve reduction of infection base, production method Simple, value-added effect

Inactive Publication Date: 2016-11-09
HUIAN KELIAN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation process of pesticides is complicated and the cost is high; and due to the use of a large amount of pesticides, the pathogenic strain of Xanthomonas rapeseed has produced drug resistance, and the therapeutic effect of pesticides is constantly declining; in addition, a large amount of pesticides remain in radish Harmful substances that will affect human health and cause waste of resources

Method used

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  • Application of rhizoma smilacis glabrae extract for preventing and treating radish black rot
  • Application of rhizoma smilacis glabrae extract for preventing and treating radish black rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Inhibition experiment of the extracts of Smilax smilax stem, leaf and root on Xanthomonas rape pathogenic variety.

[0017] 1. Experimental materials:

[0018] Wash and decompose Smilax smilax into roots, stems, and leaves, and dry them in an oven at 40°C for 6 hours, and crush the dried Smilax smilax stem tissue with a plant grinder; Mix solvents with a ratio of 1:7, soak and extract for 3 times, then filter and remove residue, soak for 12 hours each time, and soak at a temperature of 50°C; the soaked extracts are respectively concentrated under reduced pressure at a temperature of 55°C to make:

[0019] (1) Smilax smilax stem extract extract;

[0020] (2) Smilax tuckahoe leaf extract extract;

[0021] (3) Smilax root extract extract.

[0022] 2. Experimental method:

[0023] Pour the experimental materials (1)-(3) into the PDA medium with a diameter of 75 mm, spread them evenly with a triangular glass rod, and then use a hole punch with a diameter of 12 mm to prep...

Embodiment 2

[0027] Wash the stems of Smilax smilax, dry in an oven at 30°C for 5 hours, crush them with a plant grinder, add them to an ethanol solvent with a mass-to-volume ratio of 1:5, soak and extract 3 times, filter and remove slag, and each extraction time is 5 hours, the extraction temperature is 30°C; the extract is concentrated under reduced pressure at a temperature of 40°C to prepare the extract of Smilax smilax stem extract.

[0028] Incidence grading standards: Grade 0, no disease on the whole plant; Grade 1, brown spot lesions appear; Grade 3, typical spindle-shaped lesions appear, and the lesion area accounts for less than 5% of the whole leaf area; Grade 5, typical lesions, Lesion area accounts for 6%-25% of the whole leaf area; Grade 7 typical lesion area accounts for 26%-50% of the entire leaf area; Grade 9, typical lesion area accounts for 50% of the entire leaf area %above.

[0029]

[0030]

[0031] The control effect of the Smilax smilax stem extract extract m...

Embodiment 3

[0034] Wash the stems of Smilax smilax, dry in an oven at 50°C for 8 hours, crush them with a plant grinder, add them to an ethanol solvent with a mass-volume ratio of 1:10, soak and extract 3 times, filter and remove slag, and the extraction time is 48 hours each time. hours, the extraction temperature is 80°C; the extract is concentrated under reduced pressure at a temperature of 70°C to prepare the extract of Smilax smilax stem extract.

[0035] The preventive effect of the Smilax smilax stem extract extract made in Example 3 to radish black rot

[0036] Dosage of test drug

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Abstract

The invention discloses application of a rhizoma smilacis glabrae extract for preventing and treating radish black rot. The method comprises the steps that firstly, a rhizoma smilacis glabrae stem extract is prepared, and in the flowering phase of radish, a rhizoma smilacis glabrae stem extract diluent is sprayed on radish plants at least one time; in the young fruit period of radish, a rhizoma smilacis glabrae stem extract diluent is sprayed on radish plants at least one time. On the basis of a spore restraining experiment of oilseed rape xanthomonas oilseed rape pathopoiesia varieties on extractives of different organs of rhizoma smilacis glabrae, the biological control method for preventing radish black rot is found; the method restrains propagation of oilseed rape xanthomonas oilseed rape pathopoiesia varieties through the rhizoma smilacis glabrae stem extract, radish black rot infection cardinal number is lowered, and therefore the purpose of preventing diseases is achieved. The manufacturing method is simple, low in cost, more natural and more environmentally friendly. In addition, rhizoma smilacis glabrae further has the effects of detoxification, dehumidification and easing joint movement, and the additional value of radish is remarkably added.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to the application of Smilax smilax extract in preventing and treating radish black rot. Background technique [0002] Radish black rot, commonly known as black heart and rotten heart, is a disease caused by the infection of Xanthomonas campestris pv. campestris (Pammal) Dowson. It mainly harms leaves and roots. At the seedling stage, the cotyledon leaves are water-soaked, and the root pith turns black and rots. Leaf disease occurs, and yellow spots appear in many places on the edge of the leaf, which later becomes "V" and develops inward. Both the growth period and the storage period of the radish can cause damage, and the radish loses its commodity performance, causing great losses. [0003] Incidence conditions: mainly occurs in autumn. When the temperature is 15 ℃ ~ 21 ℃, it is rainy and the condensation time is long. The disease is more severe in plots where cruciferous vege...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/40A01P3/00
CPCA01N65/40
Inventor 王少平
Owner HUIAN KELIAN AGRI TECH CO LTD
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