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A Pretreatment Method for the Simultaneous Analysis of Organic Contaminants and Metals in Hair Samples

A technology for simultaneous analysis of organic pollutants, applied in the preparation of test samples, etc., can solve the problems of less hair samples, increase costs, increase research costs, etc., to reduce costs and time costs, reliable detection accuracy, and reduce hair. The effect of sample size

Active Publication Date: 2020-06-02
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially when it is necessary to accurately study the exposure of individuals in a certain period of time, because only the hair grown in the corresponding period can be used for determination, the hair samples will become less; in addition, when it is necessary to study the environment of a specific population such as infants and young children In exposure situations, the availability of hair samples will also be very small, often difficult to meet the requirements of existing separate processing operations
[0007] In addition, sample pretreatment operations generally account for more than 60% of the analysis operations, and separate pretreatments will also increase the cost of analysis, and the sample size of environmental epidemiological studies is usually large (hundreds to hundreds of thousands), which is means a significant increase in research costs

Method used

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  • A Pretreatment Method for the Simultaneous Analysis of Organic Contaminants and Metals in Hair Samples
  • A Pretreatment Method for the Simultaneous Analysis of Organic Contaminants and Metals in Hair Samples
  • A Pretreatment Method for the Simultaneous Analysis of Organic Contaminants and Metals in Hair Samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0092] Hereinafter, the present invention will be described in detail with reference to experimental examples, examples, and comparative examples, but the present invention is not limited to these examples.

[0093] Wherein, the abbreviation of reagent used is as follows:

[0094] Tetramethylammonium Hydroxide: TMAH

[0095] Sodium Hydroxide: NaOH

[0096] n-Hexane: HEX

[0097] Dichloromethane: DCM

[0098] Chloroform: TCM

[0099] Polycyclic Aromatic Hydrocarbons: PAHs

[0100] Nicotine: NIC

[0101] Cotinine: COT

[0102] The abbreviations for each organic pollutant are as follows:

[0103] Acenaphthene: ACE

[0104] Fluorene: FLU

[0105] Philippines: PHE

[0106] Anthracene: ANT

[0107] Fluoranthene: FLO

[0108] Pyrene: PYR

[0109] Benz(a)anthracene: BAA

[0110] CHR

[0111] Benzo(b)fluoranthene: BBF

[0112] Benzo(k)fluoranthene: BKF

[0113] Benzo(a)pyrene: BAP

[0114] Indenyl (1,2,3-cd)pyrene: I1P

[0115] Dibenzo(ah)anthracene: DahA

[0116]...

experiment example 1

[0118] The collected 25mg human hair samples were shredded (cut to 3-5mm / segment), cleaned (for specific steps, see Example 1 described later), and then the organic base (TMAH, 0.5mL, 2.73M ) and the prior art inorganic base (NaOH, 0.5mL, 2.5M) were digested, and the digestion of the hair sample by the two bases was studied.

[0119] The microscopic observation results of TMAH-digested hair samples and NaOH-digested hair samples are shown in figure 2 A and figure 2 b. From figure 2 It can be seen that compared with NaOH, the hair fragments after TMAH digestion are less and very small, and its digestion effect is obviously better than that of NaOH used in the prior art.

experiment example 2

[0121] Get 25mg hair standard samples respectively (Tanmo Quality Inspection-Standard Material Center, human hair component standard material, GBW07601 (GSH-1a)), use HEX, TCM, HEX and DCM mixed solvent (volume ratio is 3:2, Recorded as HEX:DCM32,), the mixed solvent of HEX and TCM (volume ratio is 7:3, recorded as HEX:TCM73) is extracted, then use gas chromatography (Agilent, 7890B)-mass spectrometer (Agilent, 5977A) The recoveries of 15 kinds of polycyclic aromatic hydrocarbons, nicotine and cotinine in the organic layer extracted by each organic solvent were determined.

[0122] show the result in image 3 , Figure 4 ,From image 3 and Figure 4 It can be seen intuitively that mixed solvents HEX:DCM32 and HEX:TCM73 have good extraction efficiencies (both reaching more than 80%) to various polycyclic aromatic hydrocarbons, nicotine and cotinine, and when polar solvent or nonpolar When the solvent is used alone, it is often difficult to have good extraction efficiency fo...

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Abstract

The invention relates to a pretreatment method and a kit thereof for simultaneously analyzing organic pollutants and metals in a hair sample. The pretreatment method is characterized by comprising the following steps: digesting hairs by an organic alkali, extracting the organic substances from the solution by an organic solvent, and fully dissociating the metal elements in the alkali digestion solution by an inorganic acid after extraction, wherein the molecular weight of the organic alkali is about 15 to 330, and the density of mixed solvent is less than 1.

Description

technical field [0001] The invention relates to a pretreatment method and a kit for synchronously analyzing organic pollutants and metals in hair samples. Background technique [0002] The health hazards caused by environmental pollution exposure are gradually increasing in the proportion of human disease burden, among which organic pollutants and heavy metals are considered to be the main factors affecting human health. For organic pollutants, polycyclic aromatic hydrocarbons, nicotine and cotinine have received much attention. Among them, polycyclic aromatic hydrocarbons (PAHs) widely exist in the environment and are aromatic hydrocarbon compounds formed by two or more fused benzene rings, mainly derived from fossil fuels (such as coal, oil and natural gas, etc.) , biomass fuels, etc. incomplete combustion process, is atmospheric fine particulate matter (PM 2.5 ) and an important class of organic pollutants leading to adverse health outcomes. Nicotine is mainly derived ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/34
CPCG01N1/28G01N1/34
Inventor 王斌李智文李振江
Owner PEKING UNIV
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