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Method for extracting sea-buckthorn protein from sea-buckthorn seed meal

A technology of seabuckthorn protein and seabuckthorn seeds, which is applied in the field of protein extraction, can solve the problems of insufficiently high seabuckthorn protein content and darker color, and achieve the effects of improving comprehensive utilization, increasing added value, and low cost

Inactive Publication Date: 2016-10-26
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is to provide a method that can efficiently utilize the waste of seabuckthorn seed meal, and another purpose is to solve the problem that the currently extracted seabuckthorn protein content is not high enough and the color is darker, and provide at least the advantages that will be described later

Method used

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  • Method for extracting sea-buckthorn protein from sea-buckthorn seed meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Step 1: Weigh 225g of seabuckthorn seed meal and crush it for 35S in a ZN-10 small pulverizer with a power of 1.5Kw, and then pass through a 40-mesh sieve to obtain seabuckthorn seed meal fine powder;

[0033] Step two, take 10g of sieved and subdivided into a round bottom flask, add 60% ethanol solution according to 1:6 material-liquid ratio, stir and extract 3 times on a constant temperature magnetic stirrer at 50℃, each 60min, centrifuge (3000PRM, 20min), collect the precipitate and wash with water until it is neutral;

[0034] Step 3: Add water to the precipitate after washing with water according to the ratio of material to liquid at 1:12, stir and mix well, adjust the pH to 10 with 0.1 mol / L sodium hydroxide, stir and extract 3 times at 50°C, each time for 50 minutes, centrifuge at 3000PRM for 20 minutes , Collect and combine the supernatants, add 30% hydrogen peroxide solution (30% hydrogen peroxide to alkaline extract ratio 1:20) to the supernatant, stir at 45°C for ...

Embodiment 2

[0037] Step 1: Weigh 225g of sea-buckthorn seed meal and crush it for 35S in a ZN-10 small pulverizer with a power of 1.5Kw, then pass through a 60-mesh sieve to obtain sea-buckthorn seed meal fine powder;

[0038] Step two, take 10g of sieved and subdivided into a round bottom flask, add 60% ethanol solution according to 1:6 material-liquid ratio, stir and extract 3 times on a constant temperature magnetic stirrer at 50℃, each 60min, centrifuge (3000PRM, 20min), collect the precipitate;

[0039] Step 3: Add water to the precipitate after washing with water according to the ratio of material to liquid at 1:12, stir and mix well, adjust the pH to 10 with 0.1 mol / L sodium hydroxide, stir and extract 3 times at 50°C, each time for 50 minutes, centrifuge at 3000PRM for 20 minutes , Collect and combine the supernatants, add 30% hydrogen peroxide solution (30% hydrogen peroxide to alkaline extract ratio 1:20) to the supernatant, stir at 45°C for 60 minutes, and then adjust the pH to 0.1m...

Embodiment 3

[0042] Step 1: Weigh 225g of seabuckthorn seed meal and crush it for 35S in a ZN-10 small pulverizer with a power of 1.5Kw, and pass it through an 80-mesh sieve to obtain seabuckthorn seed meal fine powder;

[0043] Step two, take 10g of sieved and subdivided into a round bottom flask, add 60% ethanol solution according to 1:6 material-liquid ratio, stir and extract 3 times on a constant temperature magnetic stirrer at 50℃, each 60min, centrifuge (3000PRM, 20min), collect the precipitate.

[0044] Step 3: Add water to the precipitate after washing with water according to the ratio of material to liquid at 1:12, stir and mix well, adjust the pH to 10 with 0.1 mol / L sodium hydroxide, stir and extract 3 times at 50°C, each time for 50 minutes, centrifuge at 3000PRM for 20 minutes , Collect and combine the supernatants, add 30% hydrogen peroxide solution (30% hydrogen peroxide to alkaline extract ratio 1:20) to the supernatant, stir at 45°C for 60 minutes, and then adjust the pH to 0.1...

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Abstract

The invention discloses a method for extracting sea-buckthorn protein from sea-buckthorn seed meal. The method comprises: (1) getting sea-buckthorn seed meal and pulverizing the sea-buckthorn seed meal to obtain sea-buckthorn seed meal powder; (2) sieving the sea-buckthorn seed meal powder to obtain fine sea-buckthorn seed meal powder; (3) putting the fine sea-buckthorn seed meal powder into an alcohol solution, performing stirring, washing the fine sea-buckthorn seed meal powder, and collecting the washed precipitation; (4) washing the precipitation with water till the precipitation is neutral, adding water, performing fully mixing till the obtained solution is uniform, adjusting the pH of the obtained solution with a NaOH solution, performing stirring extraction, performing centrifugation or filtration, and collecting and combining the supernates; (5) adding a hydrogen peroxide solution into the supernates, performing stirring, adjusting the pH of the obtained solution with acid, performing standing, and collecting precipitation; and (6) washing the obtained precipitation till the precipitation is neutral and performing freeze drying to obtain sea-buckthorn protein. According to the method, sea-buckthorn seed meal is taken as a raw material, therefore, reuse of industrial waste is achieved. The method is low in cost, and the additional value is greatly raised. Therefore, through the method, the comprehensive utilization ratio of sea-buckthorn resources is greatly increased.

Description

Technical field [0001] The invention relates to the field of protein extraction, in particular to a method for extracting sea buckthorn protein from sea buckthorn seed meal. Background technique [0002] Sea-buckthorn fruit is the fruit of Hippophae rhamnoides L., a plant of the genus Elaeaceae, also known as vinegar willow fruit, sour thorn fruit. According to statistics, my country’s sea-buckthorn production accounts for more than 95% of the global total, mainly distributed in Inner Mongolia and Northwest Regions and other places. Seabuckthorn is a medicinal and edible plant with a variety of natural nutrients, such as protein, vitamins, polysaccharides, fatty acids, organic acids, polyphenols, flavonoids, and steroids. [0003] Seabuckthorn seed meal is a waste material after carbon dioxide supercritical extraction, also called seabuckthorn pomace. It has been discarded directly for a long time, causing a lot of environmental pollution, or used as animal feed, such as patent CN2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/145Y02P20/54
Inventor 龙晓燕罗学刚龙谭
Owner SOUTHWEAT UNIV OF SCI & TECH
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