Method for extracting medical material-based collagen from fish scales

A technology of original protein and fish scale, which is applied in the field of extracting medical material-based collagen, can solve the problem of not being able to maintain the natural structure of collagen, and achieve the effect of maintaining the integrity of the triple helix structure of collagen, good biocompatibility, and short use time

Inactive Publication Date: 2016-10-19
SHANGHAI FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The present invention proposes a method for extracting medical material-based collagen from fish scales, which solves the problem in the prior art that extracting collagen from fish scales cannot maintain the natural structure of collagen

Method used

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  • Method for extracting medical material-based collagen from fish scales

Examples

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Embodiment 1

[0031] Take 100g of fresh tilapia scales, rinse with 20 times 4°C water for 30min, repeat once, and drain the water.

[0032] Put fish scales into 200g decolorizer containing 5% ammonium persulfate, 50% potassium persulfate, 10% sodium persulfate, 10% silicon dioxide, 1% EDTA, 15% sodium percarbonate, 9% sodium metasilicate , then add 2% hydrogen peroxide, stir evenly, and soak at 4°C for 24hr.

[0033] Wash with deionized water 4°C 1:20 (w / w) 4 times, 30min each time, and drain the water.

[0034] Put the fish scales into 0.5mol / LEDTA solution at 4°C, the ratio of solid to liquid is 1:30 (w / w), stir for 48 hours, change the solution every 12 hours, and drain the water.

[0035] Wash with deionized water at 4°C (1:30, w / w) 4 times for 1 hr each time, and drain the water.

[0036] Put the washed fish scales into 0.5 mol / L acetic acid solution at 4°C, the ratio of solid to liquid is 1:30 (w / w), add 0.2% pepsin, and enzymatically hydrolyze and extract the gel for 24 hours.

[...

Embodiment 2

[0045] Take 200g of frozen grass carp scales, rinse with 20 times 4°C water for 30min, repeat once, and drain.

[0046] Put fish scales into 200g decolorizer containing 8% ammonium persulfate, 45% potassium persulfate, 10% sodium persulfate, 12% silicon dioxide, 2% EDTA, 15% sodium percarbonate, 8% sodium metasilicate , then add 2% hydrogen peroxide, stir evenly, and soak at 4°C for 24hr.

[0047]Wash with deionized water 4°C 1:20 (w / w) 4 times, 30min each time, and drain the water.

[0048] Put the fish scales into 0.5mol / LEDTA solution at 4°C, the ratio of solid to liquid is 1:30 (w / w), stir for 48 hours, change the solution every 12 hours, and drain the water.

[0049] Wash 4 times with deionized water at 4°C (1:30w / w), 1hr each time, and drain the water.

[0050] Put the washed fish scales into 0.5 mol / L acetic acid solution at 4°C, the ratio of solid to liquid is 1:30 (w / w), add 0.4% trypsin, and enzymatically hydrolyze and extract the gel for 24 hours.

[0051] Centri...

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Abstract

The present invention provides a method for extracting medical material-based collagen from fish scales. The method comprises: (1) washing fish scales: using a decolorizing agent and hydrogen peroxide to decolorize the fish scales, and washing the decolorized fish scales; (2) placing the fish scales in an ethylenediaminetetraacetic acid solution, stirring, changing the ethylenediaminetetraacetic acid solution every 10-15 h, and draining the water; (3) carrying out stirring washing on the fish scales; (4) placing the fish scales into an acetic acid solution, adding protease to carry out enzymolysis gel extraction, centrifugating, and taking the supernatant; (5) adding the supernatant to a saturated NaCl or (NH4)2SO4 solution, standing, centrifugating, taking the precipitated crude collagen, and carrying out dialysis purification; and (6) carrying out freezing drying. According to the present invention, with the prepared collagen, the nature structure of the collagen can be well maintained; the fish scales are subjected to the decolorizing pre-treatment, such that the melanin on the surface of the fish scales is removed, and the color of the collagen is improved; the collagen purity is improved; and with the enzymolysis collagen extraction, the collagen yield is improved.

Description

technical field [0001] The invention relates to the field of preparation of biomedical materials, in particular to a method for extracting medical material-based collagen from fish scales. Background technique [0002] Collagen is the most abundant and important structural protein in animals. It is widely distributed in the skin, bones, cartilage, teeth, tendons, ligaments, and blood vessels of all multicellular animals from aquatic animals to vertebrates. It is the main support tissue and connective tissue. Composition, accounting for about 25% of the total body protein. In animals, it plays a variety of functions such as supporting organs, protecting the body, connecting, and nourishing. Collagen has unique structural and functional characteristics: three α-polypeptide chains form a triple-helix superhelical structure through α-helices; genetically different α-polypeptide chains constitute 18 types of collagen with different structures; there are about 10% hydroxyproline ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
Inventor 何兰王南平郭休玉
Owner SHANGHAI FISHERIES RES INST
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