Disubstituted bicyclic derivative and application thereof as efflux pump inhibitor to anti-microbial

A kind of technology of reaction and compound, applied in the field of disubstituted bicyclic derivatives and preparation thereof

Inactive Publication Date: 2016-10-12
SHANDONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, research on efflux pump inhibitors for bacterial AcrB efflux pumps is a hot spot, focus and difficulty in research at home and abroad. In recent years, the discovery of new AcrB efflux pump inhibitors has rarely been reported.

Method used

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  • Disubstituted bicyclic derivative and application thereof as efflux pump inhibitor to anti-microbial
  • Disubstituted bicyclic derivative and application thereof as efflux pump inhibitor to anti-microbial
  • Disubstituted bicyclic derivative and application thereof as efflux pump inhibitor to anti-microbial

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Preparation of benzylidene succinic acid (compound of formula 2)

[0096] Benzaldehyde (15.0g, 141mmol) and dimethyl succinate (25.8g, 17.6mmol) were dissolved in ethanol (100mL), and the above mixture was slowly added dropwise to ethanol ( 150mL) solution, reflux for 4h. After the reaction was completed, evaporated to dryness under reduced pressure, the resulting solid was dissolved in 15% NaOH aqueous solution (100 mL), heated to reflux for 3 h, the reaction solution was cooled to room temperature, extracted once with ethyl acetate (60 mL), and the aqueous phase was retained. Use concentrated HCl to adjust the pH to <2, and a white solid precipitates out, which is filtered with suction, and the filter cake is washed twice with water. The filter cake was dried to obtain 21.75 g of white solid, with a yield of 75%.

Embodiment 2

[0098] Preparation of 4-hydroxyl-2-naphthoic acid (compound of formula 3)

[0099] Benzylidene succinic acid (20.6g, 100mmol) was slowly added to stirred concentrated sulfuric acid (147g, 1500mmol) at low temperature, protected by nitrogen, and then transferred to room temperature for 48h. The reaction solution was slowly added dropwise into stirred ice water (300 mL), and a yellow solid was precipitated. Suction filtration with a Buchner funnel, washing with ice water three times to obtain a crude brown-yellow solid. Finally, it was recrystallized with ethanol / water to obtain 12.2 g of a yellow solid with a yield of 65%.

Embodiment 3

[0101] Preparation of 4-acetoxy-2-naphthoic acid (compound of formula 4)

[0102] 4-Hydroxy-2-naphthoic acid (12.2g, 65mmol) was dissolved in pyridine (80mL), and acetic anhydride (26.5g, 260mmol) was slowly added dropwise at room temperature, and reacted at room temperature for 3h. After the reaction was completed, evaporate to dryness under reduced pressure, add water (120 mL) to the obtained residue, adjust the pH=3 with 1N HCl, extract three times with ethyl acetate (3×80 mL), combine the organic phases, wash the organic phases with saturated brine, Anhydrous Na 2 SO 4 After drying, filtering, and evaporating to dryness under reduced pressure, 13.3 g of brown-yellow solid was obtained, with a yield of 89%.

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PUM

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Abstract

The invention discloses a disubstituted bicyclic derivative and application thereof as an efflux pump inhibitor to anti-microbial. It is proved by experiments that the disubstituted bicyclic derivative has a good inhibition function on multidrug-resistant bacteria carrying an AcrB efflux pump and can effectively recover or enhance antibacterial efficiency of existing antibiotics. The chemical structural formula of the disubstituted bicyclic derivative is as shown in a formula (I) (the formula can be seen in specification), and R1, R2, X, Y, Z and W are as defined in the specification.

Description

technical field [0001] The invention relates to a class of disubstituted bicyclic derivatives, a preparation method thereof, and an antibacterial application as an antibacterial synergist. Background technique [0002] Due to the widespread use and even abuse of antibiotics, the problem of bacterial resistance is becoming more and more serious, and it has become one of the important factors that seriously threaten human health. In recent years, the multi-drug resistance of Gram-negative bacteria to antimicrobial agents has been increasing day by day, and multi-drug-resistant Gram-negative bacteria infection has become the main cause of death in clinical patients. The most common clinical multidrug-resistant Gram-negative bacteria include Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Acinetobacter baumannii, etc. The isolation rate of Escherichia coli in ICU wards is as high as 34%. The drug rate is as high as 44%, often causing serious infections in vari...

Claims

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Application Information

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IPC IPC(8): C07D239/94C07D403/12C07D215/48C07D307/52C07D295/088C07C235/66C07C323/36C07D233/60C07D249/08C07D235/08C07D235/28C07D277/70C07D295/192C07C231/02C07C319/20A61K31/517A61P31/04A61K31/47A61K31/4709A61K31/535A61K31/4164A61K31/4196A61K31/4184A61K31/428A61K31/41A61K31/495A61K31/165
CPCC07C235/66C07C323/36C07D215/48C07D233/60C07D235/08C07D235/28C07D239/94C07D249/08C07D277/70C07D295/088C07D295/192C07D307/52C07D403/12
Inventor 马淑涛汉丽埃塔·文特尔王印虎郭丽威鲁玛纳·莫拉
Owner SHANDONG UNIV
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