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Bionical type absorbable dura mater patch and preparation method and application thereof

A dura mater and bionic technology, which is applied in the field of bionic absorbable dura mater patch and its preparation, can solve the problems of biomimetic and meningeal composition biomimetic, and achieve good biocompatibility, suture-free fixation, and novel structure Effect

Active Publication Date: 2016-10-12
YANTAI ZHENGHAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this artificial dura mater has the following disadvantages: (1) The side facing the brain uses a hydrophobic electrospun layer, which does not contain natural hydrophilic polymers; while the meningeal layer facing the brain in the natural dura mater contains sodium hyaluronate and other endogenous lubricating substances, so the artificial dura mater failed to biomime the composition of the meningeal layer; (2) the side facing away from the brain is an isotropic cell scaffold layer, and the hydrophilic polymers inside it are arranged in disorder; However, the collagen microfibrils in the temporal region, the frontal side and the dorsal part of the natural dura-periosteal layer are highly oriented, so the artificial dura mater cannot biomime the structure of the periosteum layer.
[0006] Therefore, there is currently no artificial dura (spinal) meninga repair material that can fully mimic the natural dura mater structure, has a simple preparation process, and has broad prospects for industrialization.

Method used

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  • Bionical type absorbable dura mater patch and preparation method and application thereof
  • Bionical type absorbable dura mater patch and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Dissolve polylactic acid-glycolic acid copolymer with a weight average molecular weight of 120,000 in hexafluoroisopropanol at a concentration of 0.1 g / ml; dissolve sodium hyaluronate with a relative molecular weight of 1.2 million in deionized water at a concentration of 0.06 g / ml; a homogeneous and stable solution was formed after stirring separately at room temperature. After that, the two solutions were uniformly mixed under high-speed stirring, and the volume ratio was 95:5, which was the spinning solution a.

[0068] Put the mixed solution into a syringe, and connect the needle of the syringe to a high-voltage power supply. The solution supply flow rate was controlled at 3 ml / h, the applied voltage was 15 kV, the distance between the high-voltage end and the ground end was 15 cm, and a rotating drum with a diameter of 8 cm was used as a collection device at a speed of 100 rpm. After spinning for 5 hours, the lubricating layer fiber film ( figure 2 a).

[0069]...

Embodiment 2

[0073] Dissolve L-polylactic acid with a weight average molecular weight of 300,000 in dichloromethane at a concentration of 0.08 g / ml; dissolve carboxymethyl chitosan with a relative molecular weight of 200,000 in deionized water at a concentration of 0.02 g / ml . The two solutions were uniformly mixed under high-speed stirring, and the volume ratio was 95:5, which was the spinning solution a.

[0074] The solution supply flow rate was controlled at 1 ml / hour, the applied voltage was 12.5 kV, the distance between the high-voltage end and the ground end was 10 cm, and a rotating drum with a diameter of 8 cm was used as a collection device at a speed of 100 rpm. After spinning for 6 hours, a lubricating layer fiber film with a thickness of about 0.15 mm and disorderly arrangement can be collected.

[0075] The L-polylactic acid with a weight average molecular weight of 300,000 and the polyethylene glycol with a weight average molecular weight of 10,000 are dissolved in methylen...

Embodiment 3

[0079] The polylactic acid-caprolactone copolymer that weight-average molecular weight is 300,000 is dissolved in the mixed solution (volume ratio is 4:1) of dimethylformamide and tetrahydrofuran, and concentration is 0.12 g / ml; Sulfate sodium was dissolved in deionized water at a concentration of 0.01 g / ml. Finally, the two solutions are uniformly mixed under high-speed stirring, and the volume ratio is 97:3, which is the spinning solution a.

[0080] The solution supply flow rate was controlled at 1.5 ml / hour, the applied voltage was 15 kV, the distance between the high-voltage end and the ground end was 12.5 cm, and a rotating drum with a diameter of 8 cm was used as a collection device at a speed of 100 rpm. After 7 hours of spinning, the lubricating layer fiber film with a thickness of about 0.25 mm and disorderly arrangement can be collected.

[0081] The polylactic acid-caprolactone copolymer with a weight average molecular weight of 300,000 and the carbomer with a wei...

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Abstract

The invention discloses a bionical type absorbable dura mater patch and a preparation method and application thereof. The bionical type absorbable dura mater patch has a two-layer structure, the side making contact with the brain tissue is a lubricating layer which contains natural polymers with a lubricating effect, and the side deviating from the brain tissue is a dura mater regeneration promoting layer in which nanofibers are arranged in a highly-oriented mode. According to the bionical type absorbable dura mater patch, the microscopic structure is completely bionic to that of the natural dura mater, directional crawling of cells can be guided, the brain tissue can be protected against wearing and injuries, and tissue adhesion is prevented; meanwhile, the biocompatibility, the flexibility and the attaching property are good, industrialized production is easy to achieve, and good application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a bionic absorbable dura mater patch and its preparation method and application. Background technique [0002] The dura mater is a layer of connective tissue that lines the inside of the skull and the outside of the brain and acts as a natural barrier to protect brain tissue. Open skull injuries, meningiomas, and other brain diseases can cause dural defects, which in turn lead to cerebrospinal fluid leakage. If it is not treated, it will lead to intracranial infection, arachnoiditis or meningitis, and even develop into a series of life-threatening complications such as brain abscess and intracranial hemorrhage. At present, the main methods used clinically to repair dural defects are mostly biological patches obtained from acellular allogeneic or animal-derived dermis, pericardium, or non-degradable polymers such as polyurethane and expanded polytetrafluo...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/58A61F2/02
CPCA61F2/0063A61F2/02A61F2210/0076A61L27/50A61L27/58A61L2430/00
Inventor 窦源东孙先昌尹晓彤
Owner YANTAI ZHENGHAI BIO TECH
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