Application of compound DY1 in preparation of anti-colorectal cancer drugs
A technology of anticancer drugs and compounds, applied in the field of biomedicine
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[0025] Example 1 Inhibitory effect of compound DY1 on human colon cancer cell line SW620
[0026] 1. Select SW620 adherent cells in the logarithmic growth phase, digest with trypsin, and use DMEM medium containing 10% fetal bovine serum to prepare 1.25×10 5 cells / ml of cell suspension, inoculated in 96-well culture plate, inoculated 200 μl per well, 37°C, 5% CO 2 nourish.
[0027] 2. Prepare the sample (compound DY1) mother solution, incubate and shake the sample (compound DY1) with serum-free DMEM medium to dissolve, and prepare the mother solution with a final concentration of 2mg / ml; prepare a five-fold diluted working solution, and finally The concentration is 1mg / ml, 200μg / ml, 40μg / ml, 8μg / ml, 1.6μg / ml, 320ng / ml, the positive control drug nimustine 50mg / ml mother solution is prepared, and a five-fold diluted working solution is prepared. The concentration was 1 mg / ml, 200 μg / ml, 40 μg / ml, 8 μg / ml, 1.6 μg / ml, 320 ng / ml.
[0028] 3. After 24 hours, replace with new cultu...
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