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Application of compound DY1 in preparation of anti-colorectal cancer drugs

A technology of anticancer drugs and compounds, applied in the field of biomedicine

Inactive Publication Date: 2016-10-12
北京北科德源生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no effective molecularly targeted drugs against these cell surface molecules

Method used

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  • Application of compound DY1 in preparation of anti-colorectal cancer drugs
  • Application of compound DY1 in preparation of anti-colorectal cancer drugs
  • Application of compound DY1 in preparation of anti-colorectal cancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Inhibitory effect of compound DY1 on human colon cancer cell line SW620

[0026] 1. Select SW620 adherent cells in the logarithmic growth phase, digest with trypsin, and use DMEM medium containing 10% fetal bovine serum to prepare 1.25×10 5 cells / ml of cell suspension, inoculated in 96-well culture plate, inoculated 200 μl per well, 37°C, 5% CO 2 nourish.

[0027] 2. Prepare the sample (compound DY1) mother solution, incubate and shake the sample (compound DY1) with serum-free DMEM medium to dissolve, and prepare the mother solution with a final concentration of 2mg / ml; prepare a five-fold diluted working solution, and finally The concentration is 1mg / ml, 200μg / ml, 40μg / ml, 8μg / ml, 1.6μg / ml, 320ng / ml, the positive control drug nimustine 50mg / ml mother solution is prepared, and a five-fold diluted working solution is prepared. The concentration was 1 mg / ml, 200 μg / ml, 40 μg / ml, 8 μg / ml, 1.6 μg / ml, 320 ng / ml.

[0028] 3. After 24 hours, replace with new cultu...

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Abstract

The invention provides an application of a compound DY1 in preparation of anti-colorectal cancer drugs. According to conventional literature reports, a colorectal cancer surface molecule CD58 newly discovered is researched; a small-molecular compound specifically binding to the CD58 is selected for further drug research; a targeting small-molecular drug originated from natural products is obtained through a computer-aided drug screening technology; cell biology experimental results indicate that the natural product DY1 has significantly inhibiting effect on growth of an SW620 human colorectal cancer cell line, has the IC50 value of 31.30+ / -0.88 [mu]mol, and can be further used for development of new drugs for treatment of colorectal cancer.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to the application of compound DY1 in the preparation of anti-colorectal cancer drugs. Background technique [0002] Natural products refer to the general designation of the components or their metabolites in animals, plants, insects, marine organisms and microorganisms, as well as many endogenous chemical components in humans and animals, mainly including proteins, polypeptides, amino acids, nucleic acids, various Naturally occurring chemical components such as enzymes, monosaccharides, oligosaccharides, and polysaccharides. Natural products are widely used in drug research and development. Typical drugs that have been developed include quinine, morphine, digoxin, and vincristine. Finding biologically active components and leads from natural products to study innovative drugs is one of the effective ways recognized by new drug researchers around the world, and many achievements ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/404A61K45/06A61P35/00
Inventor 郭榕张媛李晓赵勇
Owner 北京北科德源生物医药科技有限公司
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