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Method for producing Pingxiang cacai through rapid fermentation by virtue of compound bacteria

A compound bacteria and fast technology, applied in the field of food processing, can solve the problems of long pickling time of vegetables, complex metabolites of fermentation flora, and difficult control of miscellaneous bacteria, so as to shorten the fermentation time, improve the production efficiency and improve the inhibitory effect. Effect

Active Publication Date: 2016-10-12
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional method of processing Pingxiang pickled vegetables has problems such as difficult control of miscellaneous bacteria, complex metabolites of fermentation bacteria, and long pickling time of vegetables.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Take 10 g of naturally fermented sauerkraut, cut it into pieces under a sterile environment, soak it in 90 mL of sterilized water, shake it for 30 min, and add sterile water to make a 10-fold dilution. Then take 1 volume of 10-fold dilution and add 9 volumes of sterilized water, mix well to make 10 -2 Diluent, and so on, to dilute the sample to 10 -7 times. choose 10 -3 Dilution, spread on the primary screening plate medium, and invert anaerobic culture at 36°C for 48 h, take the colonies with different shapes on the plate, separate them by streaking on the medium with the same composition as the primary screening medium, and repeat the separation for 3 After the second time, the strains of pure Lactobacillus plantarum, Lactobacillus buchneri, Issaccharomyces occidentalis and Issaccharomyces orientalis were screened out and stored on a slant at -4°C as the initial fermentation strains.

[0026] (2) Prepare MRS solid and liquid culture medium respectively, divide ...

Embodiment 2

[0032] (1) Prepare LBS liquid medium, dispense it into Erlenmeyer flasks, plug them with cotton plugs, and sterilize them by high-pressure steam at 121°C for 30 minutes, then set aside.

[0033] (2) Prepare PDA liquid medium, dispense it into Erlenmeyer flasks, plug them with cotton plugs, and sterilize them by high-pressure steam at 121°C for 30 minutes, then set aside.

[0034] (3) Purchase commercially available Lactobacillus plantarum, Lactobacillus brevis, Pichia pastoris, and Saccharomyces cerevisiae, respectively activate the strains according to the plate streaking method, inoculate them on the spare liquid medium in the plate, and culture at a constant temperature of 32°C for 3 days .

[0035] (4) The activated Lactobacillus plantarum and Lactobacillus brevis were transferred to LBS liquid medium, Pichia pastoris and Saccharomyces cerevisiae strains were transferred to PDA liquid medium, and cultured at 32°C for 3 days to obtain Lactobacillus plantarum , Lactobacillu...

Embodiment 3

[0038] (1) Prepare MRS liquid medium, dispense it into Erlenmeyer flasks, plug them with cotton plugs, sterilize them by high-pressure steam at 121°C for 20 min, and set aside.

[0039](3) Prepare potato dextrose liquid culture medium, divide it into Erlenmeyer flasks, plug them with cotton plugs, and sterilize them by high-pressure steam at 121°C for 20 minutes, then set aside.

[0040] (4) Purchase commercially available Lactobacillus pentosus, Enterococcus faecalis, Issa orientalis and Saccharomyces ruckeri, respectively activate the strains according to the plate streaking method, and inoculate them on the spare liquid medium in the plate respectively, and keep the temperature at 32°C Cultured for 3 days.

[0041] (5) The activated Lactobacillus pentosus and Enterococcus faecalis were transferred to the MRS liquid medium, and the strains of Issaccharomyces orientalis and Saccharomyces ruckeri were transferred to the potato dextrose liquid medium, and cultured at a constant...

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PUM

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Abstract

The invention relates to a method for producing Pingxiang cacai through rapid fermentation by virtue of compound bacteria. Two dominant bacteria, namely lactic acid bacteria and saccharomycetes, are screened and identified from natural fermentation and vegetable twisting process. The two bacteria are taken as initial fermentation strains, cleaned vegetables are dried until the moisture content is 15-65%, the dominant lactic acid bacteria and saccharomycetes which are obtained through purification or purchased are inoculated onto the surface of the vegetables in a ratio of lactic acid bacteria to saccharomycetes of (1-6):(1-6), the inoculation quantity is 0.5-5% of the total quantity of the vegetables, uniform stirring is carried out, then the vegetables are compacted and placed into a sterilized container, the container is sealed, kept in a dark place and stored in a shady and cool environment, and the vegetables can be taken out for eating after 20-30 days. The method provided by the invention has the advantages that fermentation time is only 1 / 3 of that of the natural fermentation, so that production benefit is greatly improved; taste, colour and the like are respectively better or close to those of the naturally fermented twisted vegetables; compared with the traditional fermented pickled vegetables, nitrite content is obviously reduced, so that food safety is improved; artificial fermentation microflora is controllable, and lactic acid bacteria-saccharomycetes compound bacteria realize coordinate symbiosis, so that inhibiting effect on putrefying bacteria is improved.

Description

technical field [0001] The invention belongs to the field of food processing. Background technique [0002] The production process of traditional Pingxiang rubbing vegetables is to dry and rub fresh vegetables (cabbage, green vegetables, cucumbers, radishes, cowpeas, mustard greens, etc.) in the sun and put them in a closed tank. The vegetables are placed in layers, and some washed straws are placed at the mouth of the tank, or pressed down with clean big stones, to drive out the air in the tank, and to ferment naturally. Generally, it can be eaten after three months. [0003] Pingxiang’s Zaicai has a history of hundreds of years. It has a unique taste, dry fragrance and refreshing taste, and is deeply loved by local residents. At present, most fermented vegetables have high salt content, fermented yeast liquid and high nitrite content, and the shelf life is not long. However, no salt is added in the production process of zucchini, and the content of nitrite is low; and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L19/20C12N1/20C12N1/16C12R1/865C12R1/84C12R1/25C12R1/24C12R1/225
Inventor 李红艳邓泽元柳佳郑苗
Owner NANCHANG UNIV
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