A kind of dilution preservation solution for killing animal semen virus bacteria

Abstract

The invention provides a diluting and preserving solution for killing animal sperm viruses and bacteria. The solution is prepared from, by weight, 25-40 parts of glucose, 2.5-3 parts of citric acid, 5-8 parts of trisodium citrate, 1.2-1.3 parts of sodium bicarbonate, 1.2-1.3 parts of ethylenediaminetetraacetic acid disodium salt, 0.7-0.9 part of potassium chloride, 2.8-3 parts of trihydroxymethyl aminomethane, 0.05-0.08 part of cysteine, 0.025-0.030 part of povidone-iodine and 0-0.002 part of antibacterial peptide. The diluting and preserving solution is used for dilution, preservation and bacteria and virus killing of animal sperm.

Description

technical field [0001] The invention belongs to the field of artificial insemination of animals in animal husbandry, and in particular relates to a diluted preservation solution for killing virus and bacteria in animal semen. Background technique [0002] The advantage of artificial insemination technology is that it can collect high-quality animal semen manually, and through certain technical means, it can be stored and transported for a long time, which will bring huge economic benefits to the improvement of animal husbandry varieties and production performance. However, artificial insemination technology also has its natural defect, that is, it can infect the recipient with all the pathogenic microorganisms that may be contained in the donor's semen. Taking pigs as an example, boar semen usually contains 10 4 ~10 5 CFU / ml of bacteria and possibly contaminated unknown viruses and other pathogenic microorganisms. These bacteria and viruses will affect the storage time o...

AI Technical Summary

Problems solved by technology

The semen purification and protection agent in this literature is not suitable for iodine-containing disinfectants

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